To use Invitrogen's proprietary design algorithm, select the option button next to Default Motif Pattern. This algorithm performs a statistical analysis of the target sequence—considering such factors as sequence composition, nucleotide content at 5' and 3' ends, and thermodynamic properties—and correlates it with data collected from multiple validated siRNA oligo sets tested on different gene targets. Up to 10 designs will be generated for the selected target sequence.
Note: The Default Motif Pattern design algorithm will give the best results in most cases, and is highly recommended. The results using this algorithm may include some Tuschl's patterns; these will be noted on the results page.