In vivo-purity Stealth RNAi™ siRNA and BLOCK-iT™ siRNA duplexes are specifically formulated for use in animals. They are provided in the 2’-deprotected form and undergo additional processing to make them suitable for in vivo use immediately following resuspension. The in vivo-purity processing includes dialysis and counter-ion exchange (which replaces ammonium with sodium and reduces the overall salt concentration to physiological levels), followed by sterile filtration and an option for endotoxin testing. The in vivo-purity HPLC processing option includes everything stated above plus an HPLC ion-exchange step that results in a minimum full-length purity of 95%. The mass of each oligonucleotide is confirmed by MALDI-TOF mass spectrometry. For more information, see the user manual.
To begin ordering in vivo-purity products, select the button next to the desired product type: custom Stealth RNAi™ siRNA, Stealth Select RNAi™ siRNA, custom BLOCK-iT™ siRNA, or appropriate controls.
Click on In vivo Services Pricing Information for pricing information.
The sequence information fields will change depending on the product type you select. No sequence information is required if you select one of the controls.
Custom Stealth RNAi™ siRNA — Enter a name for the custom Stealth RNAi™ siRNA oligos that you are ordering, then enter the sense-strand sequence and antisense-strand sequence. Sequences must be 25 nucleotides in length, and the antisense sequence must complement the sense sequence. Invalid bases will be flagged for correction. The sequence should contain the following 1-letter base code abbreviations: A, C, G, U (case insensitive).
Stealth Select RNAi™ siRNA — Enter the specific ID number of the Stealth Select RNAi™ siRNA oligo you want to order. Click on Find Stealth Select IDs to search for Stealth Select RNAi™ siRNA IDs based on gene or other information.
Custom BLOCK-iT™ siRNA — Enter a name for the custom BLOCK-iT™ siRNA molecules that you are ordering, then enter the sense-strand sequence and antisense-strand sequence. Sequences must be 19 nucleotides in length, and the antisense sequence must complement the sense sequence. Invalid bases will be flagged for correction. The sequence should contain the following 1-letter base code abbreviations: A, C, G, U (case insensitive).
Select Purity — Select HPLC, Desalt in vivo purity, or HPLC in vivo purity from the drop down menu. Desalt in vivo-purity processing includes dialysis and counter-ion exchange (which replaces ammonium with sodium and reduces the overall salt concentration to physiological levels), followed by sterile filtration. HPLC in vivo purity includes all of the above plus an HPLC ion-exchange step that results in a minimum full-length purity of 95%.
Select Delivered Quantity — The available delivered quantities are different depending on the purity you select. For Desalt in vivo purity, the available delivered quantities are 25 nmole, 100 nmole, and 2 µmole. For HPLC and HPLC in vivo purity, the available delivered quantities are 5 nmole, 20 nmole, and 500 nmole.
Select 5' Sense Label — For HPLC and HPLC in vivo purity, you can select a biotin or fluorescent label for the 5' end of the sense strand.
Endotoxin testing — Select this box if you want optional endotoxin testing performed on the purified oligos.
Note: For additional RNAi ordering options, click on Contact Us at the bottom of the page
When you have made your selections, click on Add to Cart. The Order Form will be displayed.