PlateSelect™ allows you to lay out and order 96-well microplates containing Stealth RNAi™ siRNA duplexes or siRNA duplexes.
To begin using PlateSelect™, specify whether you want to create ("add") a new plate layout or import an existing plate layout.
Add Plate: To create a new plate, click on Add Plate and enter the information in the Plate Properties box. Note that a particular plate can contain either Stealth RNAi™ siRNA or siRNA duplexes. A 96-well empty plate diagram will appear.
Import Plate: If you have previously saved a PlateSelect™ plate layout as a comma-separated value (*.csv) file (see Saving the Plate Diagram below), you can import the layout back into PlateSelect™. Click on Import Plate to import the saved *.csv file.
With the plate diagram displayed, you can use the buttons above the PlateSelect™ Clipboard (displayed to the right of the plate diagram) to select the duplexes that you want to add to the plate. These selections will be placed on the Clipboard.
RNAi Express: To use RNAi Express to select duplexes, click on Add Duplexes via RNAi Express. RNAi Express will open in a new window. Search for Validated Stealth RNAi™ siRNA (bench-tested RNAi duplexes) or Stealth Select RNAi™ (predesigned RNAi duplexes). With the search results displayed, select Plate format, and then select the duplexes that you want to order. Then click on Add to Shopping Cart to add the selected duplexes to the PlateSelect™ Clipboard. Note: If you select a Validated Stealth RNAi™ siRNA, two validated duplexes will be added to the Clipboard.
RNAi Designer: To use RNAi Designer to select duplexes, click on Add Duplexes via RNAi Designer. RNAi Designer will open in a new window. Design your Stealth RNAi™ siRNA or siRNA duplexes, and in the design results window select Plate format. Then click on Add to Shopping Cart to add the selected duplexes to the PlateSelect™ Clipboard.
Custom Duplexes: Click on Add Custom Duplexes and enter the duplex information in the window. The duplex type is preselected based on the current plate type. Enter a 25-nucleotide sense sequence for Stealth RNA™ siRNA oligos or a 19-nucleotide sense sequence for siRNA oligos (the antisense sequence will be auto-filled; a 3’ dTdT overhang will be added to siRNA). Then add the duplexes to the Clipboard.
After you add duplexes to the Clipboard, you can use them to populate the plate diagram.
Moving Individual Duplexes to the Plate: To transfer an individual duplex to the plate, click and drag the duplex from the Clipboard to a plate well, and click to place it in the well.
Adding All Duplexes to the Plate: Click on Add All to Plate to add all duplexes in the Clipboard to the plate (all items on the current plate will be replaced). If there are more than 96 duplexes in the Clipboard, the first 96 duplexes are added to the plate.
Controls: Available Stealth RNAi™ siRNA-specific and siRNA-specific controls are listed in the Controls section below the Clipboard. You can 'click-drag-click individual controls to any well on the plate as described above.
Moving Duplexes Back to the Clipboard: You can click and drag individual duplexes back to the Clipboard. To move more than one item, hold down the Ctrl or Command key, click on the duplexes you want to move, and select Move to Clipboard, or right-click and select Send Selected Items to Clipboard.
Moving Duplexes to the Trash: To remove individual duplexes from the Clipboard or plate diagram, click and drag them to the Trash icon in the lower left corner of the window, or use the Ctrl-click option described above to select multiple duplexes, right-click, and select Send Selected Items to Trash.
Clear Plate: To clear the plate and place all the duplexes back on the Clipboard, click on the Clear Plate button.
Delete Plate: To delete the plate and place all the duplexes back on the Clipboard, click on the Delete Plate button.
To save the plate diagram for up to 30 days in PlateSelect™, click on the Save Plate button.
To save the plate diagram as a *.csv file on your computer that you can then upload back into PlateSelect™, click on the Download Plate button.
When you are finished configuring the plate diagram, click on Order Plate. You can order one or multiple plates at a time. The plate information will be added to the Invitrogen shopping cart (no login required) where you can see the list price. Registration and login are required to see account-specific pricing and to check out.