BLOCK-iT™ RNAi Designer
The easiest way to design effective RNAi molecules for great results
See also:
Synthetics for in vivo RNAi
Target Design Options:
Stealth RNAi™ siRNA
miR RNAi
shRNA
siRNA to Stealth RNAi™ siRNA
siRNA to shRNA
Step 1: Enter an accession number or provide a nucleotide sequence
Accession number:
OR
Nucleotide sequence:
Enter only A, C, G, T, and U. See the online Help for additional information
Step 2: If you entered an accession number in Step 1, select regions for target design
Open reading frame (ORF)
5' UTR
3' UTR
Step 3: Choose database for Blast
Human - Homo sapiens
Mouse - Mus musculus
Rat - Rattus norvegicus
Cattle - Bos taurus
Pig - Sus scrofa
Dog - Canis familiaris
Frog - Xenopus laevis
Chicken - Gallus gallus
Fruitfly - Drosophila melanogaster
Worm - Caenorhabditis elegans
Zebrafish - Danio rerio
Mosquito - Anopheles gambiae
No blast
NOTE:
BLAST is used to compare input sequence with sequences in the database to find unique regions against which to design RNAi targets. The databases contain representative gene sequences for that species. Blast databases were updated on March 23, 2013 and the design output reflects the most up-to-date designs.
Step 4: Choose minimum and maximum G/C percentage
Minimum G/C percentage:
20%
25%
30%
35%
40%
45%
50%
55%
60%
65%
70%
75%
80%
85%
90%
Maximum G/C percentage:
20%
25%
30%
35%
40%
45%
50%
55%
60%
65%
70%
75%
80%
85%
90%
Step 5: Select siRNA design options and click "RNAi Design" to design siRNA.
Default motif pattern:
P: Proprietary (Recommended)
Tuschl's motif pattern:
A: AA(N19)TT
B: NA(N19)NN
C: NA(RN17Y)NN
D: NA(N18Y)NN
Guarantee:
The BLOCK-iT™ RNAi Designer is such an effective tool for design of siRNAs that if you order the two best siRNA sequences designed by the BLOCK-iT™ RNAi Designer, we guarantee that one of them will give greater than 70% knockdown of mRNA, given that transfection efficiency in your experiment is at least 80%.
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