BLOCK-iT™ RNAi Designer

The easiest way to design effective RNAi molecules for great results

See also001:

Synthetics for in vivo RNAi

Target Design Options:

Stealth RNAi™ siRNA

miR RNAi

shRNA

siRNA to Stealth RNAi™ siRNA

siRNA to shRNA

Step 1: Enter an accession number or provide a nucleotide sequence

Accession number:

Nucleotide sequence: Enter only A, C, G, T, and U. See the online Help for additional information

Step 2: If you entered an accession number in Step 1, select regions for target design

Open reading frame (ORF)

5' UTR

3' UTR

Step 3: Choose database for Blast

NOTE: BLAST is used to compare input sequence with sequences in the database to find unique regions against which to design RNAi targets. The databases contain representative gene sequences for that species. Blast databases were updated on March 23, 2013 and the design output reflects the most up-to-date designs.

Step 4: Choose minimum and maximum G/C percentage

Minimum G/C percentage:

Maximum G/C percentage:

Step 5: Click "RNAi Design" to design your Stealth RNAi™ siRNA molecules.

Guarantee: The BLOCK-iT™ RNAi Designer is such an effective tool for the design of Stealth RNAi™ siRNA if you order the three best Stealth RNAi™ siRNA sequences designed by the BLOCK-iT™ RNAi Designer, we guarantee that two of them will give greater than 70% knockdown of mRNA, given that transfection efficiency in your experiment is at least 80%.