siRNA

 

Invitrogen offers standard siRNA with the following options:

The turn-around time for single-stranded desalted is 5-7 days and HPLC-purified is 7-9 days. The turn-around time for double-stranded desalted is 6-8 days and HPLC-purified is 8-10 days.

For additional product information, click BLOCK-iT™ siRNA and FAQs.

siRNA Design

The BLOCK-iT™ RNAi Designer helps you find gene-specific targets and design 21-nucleotide siRNA. When you input an accession number or a nucleotide sequence, the designer:

·         Checks input sequence for SNPs (if input is a RefSeq accession number)

·         BLAST input sequence against databases and selects a number of unique regions

·         Uses proprietary design rules* to find 21 nucleotide gene-specific targets for RNAi analysis and reports up to 10 top scoring siRNA targets. You have an option to select two different motif patterns for siRNA design:

Default motif pattern: If you select ‘Default motif pattern’, the RNAi Designer finds all 19 nt target sequences that fulfill proprietary design criteria and reports up to top 10 high scoring target sequences. You can order siRNA oligos for selected target sequences via the RNAi Designer. The results using ‘Default motif pattern’ may contain Tuschl’s patterns, but if you are specifically looking for Tuschl’s patterns, select ‘Tushcl’s motif Pattern’ as your option.

Tuschl’s motif pattern: If you select ‘Tuschl’s motif pattern’, the RNAi Designer finds 19 nt target sequences that fulfill the selected pattern (nucleotide composition of 19 nt and –1, -2 and 20, 21 base position composition on the target sequence) and proprietary design criteria. The designer reports up to 10 top scoring target sequences and you can order siRNA oligos for selected target sequences via the designer. We recommend using ‘Default motif pattern’ over ‘Tuschl’s motif pattern’, as the stringency of selection is much higher when ‘Default motif pattern’ is selected.

References:

·         Elbashir SM et al. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:494-498.

·         Elbahir SM et al. (2001). Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate. EMBO J. 20:6877-6888.

·         Elbashir SM et al. (2002). Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods. 26:199-213

See Online Help for additional information.

siRNA Ordering

 

You can order siRNA via the RNAi Designer and check ‘Custom Primer Resources’ for other ordering options.

 

Proprietary design rules*: The proprietary design rules are derived based on statistical analysis of RNA experimental data (e.g. sequence composition, nucleotide content at 5' and 3' ends, thermodynamic properties) collected from multiple validated (functional and non-functional) RNA oligo sets tested on different gene targets.