Size How provided Delivery Validated Stealth RNAi™ siRNA 20 nmole each Lyophilized Synthesized upon order- 7-10 days (US and Canada) and 10-15 days (Europe and Asia Pacific). Stealth Select RNAi™ siRNA 20 nmole each Lyophilized: three non-overlapping sequences in separate tubes Synthesized upon order- 7-10 days (US and Canada) and 10-15 days (Europe and Asia Pacific). BLOCK-iT™ Pol II miR Validated miRNA Vector DuoPaks 10 μg each 20 μl solution at 500 ng/μl in TE: two vectors with non-overlapping miR RNAi sequences in separate tubes Product in inventory- standard catalog item shipping BLOCK-iT™ miR RNAi Select 50 nmole (synthesis scale) for each DNA oligo (2 oligos per BLOCK-iT™ miR RNAi Select hair pin) Lyophilized: Each BLOCK-iT™ miR RNAi Select hairpin is provided as 2 DNA oligos in separate 2-ml microcentrifuge tubes, which are ready for annealing and cloning. Each BLOCK-iT™ miR RNAi Select Set includes 4 miR RNAi Select hairpins targeting non-overlapping regions of the target mRNA (for a total of 8 DNA oligos in separate tubes) Synthesized upon order - 3 days (US and Canada) and 6-8 days (Europe and Asia-Pacific)   100% Guarantee* Validated Stealth RNAi™ siRNA (100% performance guarantee) When you purchase a Validated Stealth RNAi™ siRNA you will get at least 70% knockdown of the target mRNA with when transfected at 50 nM or greater concentration, transfection efficiency is at least 80%, and target mRNA is quantified at 48 hours post-transfection. Transfection efficiency can be monitored using a probe such as BLOCK-iT™ Fluorescent Oligo or proven to work as measured by an endogenous message, such as the p53 control included in the BLOCK-iT™ RNAi Basic Control Kit. We also recommend the use of an appropriate negative control, such as one of the three Stealth RNAi™ siRNA Negative Controls to normalize message knockdown. Stealth Select RNAi™ siRNA When you purchase a Stealth Select RNAi™ 3 siRNA Set you will get at least 70% knockdown of the target mRNA with at least two independent, non-overlapping sequences when transfected at 50 nM or greater concentration, transfection efficiency is at least 80%, and target mRNA is quantified at 48 hours post-transfection. Transfection efficiency can be monitored using a probe such as BLOCK-iT™ Fluorescent Oligo or proven to work as measured by an endogenous message, such as the p53 control included in the BLOCK-iT™ RNAi Basic Control Kit. We also recommend the use of an appropriate negative control, such as one of the three Stealth RNAi™ siRNA Negative Controls to normalize message knockdown. BLOCK-iT™ Pol II miR Validated miRNA Vector DuoPaks (100% performance guarantee) When you purchase a BLOCK -iT™ Pol II miR Validated miRNA Vector DuoPak you will get at least 70% knockdown of the target mRNA with two independent plasmids with pre-miRNA inserts designed to non-overlapping target sites when transfected such that transfection efficiency is at least 80%, and target mRNA is quantified at 48 hours post-transfection. Transfection efficiencies can be estimated by detection of EmGFP fluorescence in whole cells or by knockdown measurements achieved with the positive control vectors against endogenous or stably expressed target genes. We also recommend the use of the negative control included with each Validated Vector DuoPak.   BLOCK-iT™ miR RNAi Select When you purchase a BLOCK-iT™ miR RNAi Select 4 set and independently clone each hair pin into a BLOCK-iT™ Pol II miR RNAi Expression Vector (available separately) you will get at least 70% knockdown of the target mRNA with two of the clones when transfected such that transfection efficiency is at least 80% and target mRNA is quantified at 48 hours post-transfection. Transfection efficiencies can be estimated by detection of EmGFP fluorescence in whole cells or by knockdown measurements achieved with the positive control vectors (available separately) against endogenous or stably expressed target genes. We also recommend the use of the negative controls which are included in the BLOCK-iT™ Pol II miR RNAi Expression Vector Kits. *100% guarantee: Note 1: Transfection efficiencies will vary by cell type and transfection efficiencies in specific cell lines are not guaranteed due to experimental variation. Cell line specific transfection protocolas recommended by Invitrogen can be found here: Optimized Transfection Protocols. Note 2: When you purchase either a Validated Stealth RNAi™ siRNA or a Stealth Select RNAi™ 3 siRNA Set you will get at least 70% knockdown of the target mRNA with two independent, non-overlapping sequences when transfected at 50 nM or greater concentration, transfection efficiency is at least 80%, and target mRNA is quantified at 48 hours post-transfection. Transfection efficiency can be monitored using a probe such as BLOCK-iT™ Fluorescent Oligo or the BLOCK-iT™ Alexa Flour Red Fluorescent Control or proven to work as measured by an endogenous message, such as the p53 control included in the BLOCK-iT™ RNAi Basic Control Kit. We also recommend the use of an appropriate negative control, such as one of the three Stealth RNAi™ siRNA Negative Controls to normalize message knockdown.