Invitrogen

CD3 Monoclonal Antibody (OKT3), Biotin, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

56 Published Figures

55 References

View all (504) CD3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FIGURE: 1 / 58

CD3 Antibody (13-0037-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 605 and 0.03 µg of Biotin Mouse IgG2a K Isotype Control (Product # 13-4724-85) (left) or 0.03 µg of Anti-Human CD3 FITC (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD3 Antibody in Immunocytochemistry (ICC/IF)

CD3 Antibody in Immunohistochemistry (IHC)

CD3 Antibody in Functional Assay (Functional)

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone OKT3 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes (middle plot). Additional analysis of lymphocytes shows that CD3 clone OKT3 does not stain any CD19+ B cells (right plot). Details: Normal human whole blood was surface stained with CD3 (clone OKT3) and CD19 (clone HIB19). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used to compare CD3 staining (middle plot). Cells in the lymphocyte gate were used to compare CD3 and CD19 staining (right plot). {RE}

Product Details

13-0037-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

0.06 µg/test

View 33 publications 33 publications

Functional Assay (Functional)

View 4 publications 4 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

T-Cell Activation (TCA)

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT3

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1234955

Product Specific Information

Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: This OKT3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Heritable vaginal bacteria influence immune tolerance and relate to early-life markers of allergic sensitization in infancy.

Cell reports. Medicine

McCauley KE,Rackaityte E,LaMere B,Fadrosh DW,Fujimura KE,Panzer AR,Lin DL,Lynch KV,Halkias J,Mendoza VF,Burt TD,Bendixsen C,Barnes K,Kim H,Jones K,Ownby DR,Johnson CC,Seroogy CM,Gern JE,Boushey HA,Lynch SV

13-0037-82 was used in Flow cytometry/Cell sorting to investigate if bacterial and atopy heritability are intimately linked.

Tue Aug 16 00:00:00 EDT 2022

Atypical sideways recognition of CD1a by autoreactive γδ T cell receptors.

Nature communications

Wegrecki M,Ocampo TA,Gunasinghe SD,von Borstel A,Tin SY,Reijneveld JF,Cao TP,Gully BS,Le Nours J,Moody DB,Van Rhijn I,Rossjohn J

13-0037-82 was used in Flow cytometry/Cell sorting to show that CD1a is a ligand for Vδ1+ γδ T cells.

Tue Jul 05 00:00:00 EDT 2022

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease.

The Journal of experimental medicine

Agudelo M,Palus M,Keeffe JR,Bianchini F,Svoboda P,Salát J,Peace A,Gazumyan A,Cipolla M,Kapoor T,Guidetti F,Yao KH,Elsterová J,Teislerová D,Chrdle A,Hönig V,Oliveira T,West AP,Lee YE,Rice CM,MacDonald MR,Bjorkman PJ,Růžek D,Robbiani DF,Nussenzweig MC

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Mon May 03 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.

The Journal of biological chemistry

Tewari R,Shayahati B,Fan Y,Akimzhanov AM

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Functional assay

Thu Aug 26 00:00:00 EDT 2021

NAD⁺ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.

Cell reports

Wang Y,Wang F,Wang L,Qiu S,Yao Y,Yan C,Xiong X,Chen X,Ji Q,Cao J,Gao G,Li D,Zhang L,Guo Z,Wang R,Wang H,Fan G

13-0037-82 was used in Immunohistochemistry to study the role of NAD+ in T cell activation and tumor-inflitrating lymphocytes.

Tue Aug 10 00:00:00 EDT 2021

Allergen-specific IgE levels and the ability of IgE-allergen complexes to cross-link determine the extent of CD23-mediated T-cell activation.

The Journal of allergy and clinical immunology

Villazala-Merino S,Rodriguez-Dominguez A,Stanek V,Campion NJ,Gattinger P,Hofer G,Froeschl R,Fae I,Lupinek C,Vrtala S,Breiteneder H,Keller W,Perkmann T,Nakamura R,Pickl WF,Valenta R,Eckl-Dorna J,Niederberger V

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

IL-10 Restores MHC Class I Expression and Interferes With Immunity in Papillary Thyroid Cancer With Hashimoto Thyroiditis.

Endocrinology

Lu ZW,Hu JQ,Liu WL,Wen D,Wei WJ,Wang YL,Wang Y,Liao T,Ji QH

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro.

Journal of Cancer

Hu JQ,Lei BW,Wen D,Ma B,Zhang TT,Lu ZW,Wei WJ,Wang YL,Wang Y,Li DS,Ji QH,Liao T

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Immunohistochemistry

Mon Sep 28 00:00:00 EDT 2020

Human Liver Memory CD8⁺ T Cells Use Autophagy for Tissue Residence.

Cell reports

Swadling L,Pallett LJ,Diniz MO,Baker JM,Amin OE,Stegmann KA,Burton AR,Schmidt NM,Jeffery-Smith A,Zakeri N,Suveizdyte K,Froghi F,Fusai G,Rosenberg WM,Davidson BR,Schurich A,Simon AK,Maini MK

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in T-Cell Activation

Tue Jan 21 00:00:00 EST 2020

Small-molecule MMP2/MMP9 inhibitor SB-3CT modulates tumor immune surveillance by regulating PD-L1.

Genome medicine

Ye Y,Kuang X,Xie Z,Liang L,Zhang Z,Zhang Y,Ma F,Gao Q,Chang R,Lee HH,Zhao S,Su J,Li H,Peng J,Chen H,Yin M,Peng C,Yang N,Wang J,Liu J,Liu H,Han L,Chen X

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Thu Feb 06 00:00:00 EST 2020

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Cytotoxic T cells swarm by homotypic chemokine signalling.

eLife

Galeano Niño JL,Pageon SV,Tay SS,Colakoglu F,Kempe D,Hywood J,Mazalo JK,Cremasco J,Govendir MA,Dagley LF,Hsu K,Rizzetto S,Zieba J,Rice G,Prior V,O'Neill GM,Williams RJ,Nisbet DR,Kramer B,Webb AI,Luciani F,Read MN,Biro M

13-0037 was used in Flow cytometry/Cell sorting to reveal independent emergent behaviour in Cytotoxic T lymphocytes populations attacking tumour masses.

Tue Oct 13 00:00:00 EDT 2020

Methylation of a CGATA element inhibits binding and regulation by GATA-1.

Nature communications

Yang L,Chen Z,Stout ES,Delerue F,Ittner LM,Wilkins MR,Quinlan KGR,Crossley M

13-0037 was used in Flow cytometry/Cell sorting to show that the erythroid transcription factor GATA-1 - that typically binds T/AGATA sites - can also recognise CGATA elements, but only if the CpG dinucleotide is unmethylated.

Fri May 22 00:00:00 EDT 2020

Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4⁺ T Cell Immunity.

Cell

Binnewies M,Mujal AM,Pollack JL,Combes AJ,Hardison EA,Barry KC,Tsui J,Ruhland MK,Kersten K,Abushawish MA,Spasic M,Giurintano JP,Chan V,Daud AI,Ha P,Ye CJ,Roberts EW,Krummel MF

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Thu Apr 18 00:00:00 EDT 2019

Integrating Ligand-Receptor Interactions and In Vitro Evolution for Streamlined Discovery of Artificial Nucleic Acid Ligands.

Molecular therapy. Nucleic acids

Zumrut HE,Batool S,Argyropoulos KV,Williams N,Azad R,Mallikaratchy PR

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Fri Sep 06 00:00:00 EDT 2019

Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.

Stem cells international

Li C,Huang J,Zhu H,Shi Q,Li D,Ju X

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

Lv M,Li Z,Liu J,Lin F,Zhang Q,Li Z,Wang Y,Wang K,Xu Y

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Synthetic TRuC receptors engaging the complete T cell receptor for potent anti-tumor response.

Nature communications

Baeuerle PA,Ding J,Patel E,Thorausch N,Horton H,Gierut J,Scarfo I,Choudhary R,Kiner O,Krishnamurthy J,Le B,Morath A,Baldeviano GC,Quinn J,Tavares P,Wei Q,Weiler S,Maus MV,Getts D,Schamel WW,Hofmeister R

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Western Blot

Tue May 07 00:00:00 EDT 2019

Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy.

Cell reports

Celis-Gutierrez J,Blattmann P,Zhai Y,Jarmuzynski N,Ruminski K,Grégoire C,Ounoughene Y,Fiore F,Aebersold R,Roncagalli R,Gstaiger M,Malissen B

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane.

Cells

Rossatti P,Ziegler L,Schregle R,Betzler VM,Ecker M,Rossy J

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Mon Nov 04 00:00:00 EST 2019

TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions.

Cell reports

Leng T,Akther HD,Hackstein CP,Powell K,King T,Friedrich M,Christoforidou Z,McCuaig S,Neyazi M,Arancibia-Cárcamo CV,Hagel J,Powrie F,Peres RS,Millar V,Ebner D,Lamichhane R,Ussher J,Hinks TSC,Marchi E,Willberg C,Klenerman P

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Immunocytochemistry

Tue Sep 17 00:00:00 EDT 2019

Tumor necrosis factor induces rapid down-regulation of TXNIP in human T cells.

Scientific reports

Levring TB,Kongsbak-Wismann M,Rode AKO,Al-Jaberi FAH,Lopez DV,Met Ö,Woetmann A,Bonefeld CM,Ødum N,Geisler C

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Western Blot

Wed Nov 13 00:00:00 EST 2019

"Waltz" of Cell Membrane-Coated Nanoparticles on Lipid Bilayers: Tracking Single Particle Rotation in Ligand-Receptor Binding.

ACS nano

Yu Y,Gao Y,Yu Y

13-0037 was used in Flow cytometry to demonstrate the promise of using combined rotational and translational single particle tracking to resolve biological interactions that could be "hidden" in translational measurements alone.

Wed Dec 26 00:00:00 EST 2018

Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy.

International journal of nanomedicine

Pan H,Liu J,Deng W,Xing J,Li Q,Wang Z

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Mon Jul 02 00:00:00 EDT 2018

Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.

Cell

Lim AI,Li Y,Lopez-Lastra S,Stadhouders R,Paul F,Casrouge A,Serafini N,Puel A,Bustamante J,Surace L,Masse-Ranson G,David E,Strick-Marchand H,Le Bourhis L,Cocchi R,Topazio D,Graziano P,Muscarella LA,Rogge L,Norel X,Sallenave JM,Allez M,Graf T,Hendriks RW,Casanova JL,Amit I,Yssel H,Di Santo JP

13-0037 was used in Flow cytometry/Cell sorting to identify circulating and tissue ILC precursors in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration.

Thu Mar 09 00:00:00 EST 2017

Astrocytic tight junctions control inflammatory CNS lesion pathogenesis.

The Journal of clinical investigation

Horng S,Therattil A,Moyon S,Gordon A,Kim K,Argaw AT,Hara Y,Mariani JN,Sawai S,Flodby P,Crandall ED,Borok Z,Sofroniew MV,Chapouly C,John GR

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Immunofluorescence

Tue Aug 01 00:00:00 EDT 2017

Sorting nexin 27 interactome in T-lymphocytes identifies zona occludens-2 dynamic redistribution at the immune synapse.

Traffic (Copenhagen, Denmark)

Tello-Lafoz M,Martínez-Martínez G,Rodríguez-Rodríguez C,Albar JP,Huse M,Gharbi S,Merida I

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in T-Cell Activation

Tue Aug 01 00:00:00 EDT 2017

NKG2D Ligand-Targeted Bispecific T-Cell Engagers Lead to Robust Antitumor Activity against Diverse Human Tumors.

Molecular cancer therapeutics

Godbersen C,Coupet TA,Huehls AM,Zhang T,Battles MB,Fisher JL,Ernstoff MS,Sentman CL

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Restimulation-induced T-cell death through NTB-A/SAP signaling pathway is impaired in tuberculosis patients with depressed immune responses.

Immunology and cell biology

Hernández Del Pino RE,Pellegrini JM,Rovetta AI,Peña D,Álvarez GI,Rolandelli A,Musella RM,Palmero DJ,Malbran A,Pasquinelli V,García VE

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

A human immunodeficiency syndrome caused by mutations in CARMIL2.

Nature communications

Schober T,Magg T,Laschinger M,Rohlfs M,Linhares ND,Puchalka J,Weisser T,Fehlner K,Mautner J,Walz C,Hussein K,Jaeger G,Kammer B,Schmid I,Bahia M,Pena SD,Behrends U,Belohradsky BH,Klein C,Hauck F

13-0037-82 was used in Functional assays to show that human CARMIL2-deficiency is associated with defective CD28-mediated TCR co-signalling and impaired cytoskeletal dynamics.

Mon Jan 23 00:00:00 EST 2017

Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis.

PloS one

Geißler K,Markwart R,Requardt RP,Weigel C,Schubert K,Scherag A,Rubio I,Guntinas-Lichius O

13-0037 was used in Flow cytometry/Cell sorting to analyse and characterise the response of tonsillar T-cells from chronic tonsillitis to antigenic stimulation.

Mon Oct 16 00:00:00 EDT 2017

Functional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination.

Proceedings of the National Academy of Sciences of the United States of America

Pageon SV,Tabarin T,Yamamoto Y,Ma Y,Nicovich PR,Bridgeman JS,Cohnen A,Benzing C,Gao Y,Crowther MD,Tungatt K,Dolton G,Sewell AK,Price DA,Acuto O,Parton RG,Gooding JJ,Rossy J,Rossjohn J,Gaus K

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Immunofluorescence

Tue Sep 13 00:00:00 EDT 2016

Obesity decreases B cell responses in young and elderly individuals.

Obesity (Silver Spring, Md.)

Frasca D,Ferracci F,Diaz A,Romero M,Lechner S,Blomberg BB

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in T-Cell Activation

Tue Mar 01 00:00:00 EST 2016

CD16⁺ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.

Frontiers in immunology

Zhu H,Hu F,Sun X,Zhang X,Zhu L,Liu X,Li X,Xu L,Shi L,Gan Y,Su Y

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in T-Cell Activation

Wed Nov 20 00:00:00 EST 2019

Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.

Stem cells international

Chen J,Wang Q,Feng X,Zhang Z,Geng L,Xu T,Wang D,Sun L

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

T-lymphocyte passive deformation is controlled by unfolding of membrane surface reservoirs.

Molecular biology of the cell

Guillou L,Babataheri A,Saitakis M,Bohineust A,Dogniaux S,Hivroz C,Barakat AI,Husson J

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Immunomicroscopy

Mon Nov 07 00:00:00 EST 2016

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.

Oncotarget

You Y,Tan JX,Dai HS,Chen HW,Xu XJ,Yang AG,Zhang YJ,Bai LH,Bie P

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Tue Aug 30 00:00:00 EDT 2016

CD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b⁺ T cell lymphoma.

Oncoimmunology

Bagchi S,Li S,Wang CR

13-0037 was used in Flow cytometry/Cell sorting to demonstrate the antitumor potential of CD1b-autoreactive T cells and their potential use in adoptive immunotherapy.

Tue Jan 12 00:00:00 EST 2021

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway.

eLife

Kumari S,Depoil D,Martinelli R,Judokusumo E,Carmona G,Gertler FB,Kam LC,Carman CV,Burkhardt JK,Irvine DJ,Dustin ML

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Miscellaneous

Wed Mar 11 00:00:00 EDT 2015

Actin foci facilitate activation of the phospholipase C-γ in primary T lymphocytes via the WASP pathway.

eLife

Kumari S,Depoil D,Martinelli R,Judokusumo E,Carmona G,Gertler FB,Kam LC,Carman CV,Burkhardt JK,Irvine DJ,Dustin ML

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Miscellaneous

Wed Mar 11 00:00:00 EDT 2015

F-actin flow drives affinity maturation and spatial organization of LFA-1 at the immunological synapse.

The Journal of cell biology

Comrie WA,Babich A,Burkhardt JK

13-0037 was used in Functional assays to investigate the role of LFA-1 at the immunological synapse.

Mon Feb 16 00:00:00 EST 2015

A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.

Blood cancer journal

Kim SY,Theunissen JW,Balibalos J,Liao-Chan S,Babcock MC,Wong T,Cairns B,Gonzalez D,van der Horst EH,Perez M,Levashova Z,Chinn L,D'Alessio JA,Flory M,Bermudez A,Jackson DY,Ha E,Monteon J,Bruhns MF,Chen G,Migone TS

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Fri May 29 00:00:00 EDT 2015

Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.

Molecular cell

Guo YE,Riley KJ,Iwasaki A,Steitz JA

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Western Blot

Thu Apr 10 00:00:00 EDT 2014

Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.

Proceedings of the National Academy of Sciences of the United States of America

Kumaresan PR,Manuri PR,Albert ND,Maiti S,Singh H,Mi T,Roszik J,Rabinovich B,Olivares S,Krishnamurthy J,Zhang L,Najjar AM,Huls MH,Lee DA,Champlin RE,Kontoyiannis DP,Cooper LJ

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Tue Jul 22 00:00:00 EDT 2014

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.

PloS one

Reichwald K,Jørgensen TZ,Tougaard P,Skov S

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Flow Cytometry

Mon Sep 08 00:00:00 EDT 2014

N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.

The Journal of biological chemistry

Mellergaard M,Skovbakke SL,Schneider CL,Lauridsen F,Andresen L,Jensen H,Skov S

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in T-Cell Activation

Fri Jul 18 00:00:00 EDT 2014

Ligand mobility modulates immunological synapse formation and T cell activation.

PloS one

Hsu CJ,Hsieh WT,Waldman A,Clarke F,Huseby ES,Burkhardt JK,Baumgart T

13-0037 was used in Functional assays to investigate the effects of apparent long-range ligand mobility on T cell signalling activity and immunological synapse formation.

Mon Aug 27 00:00:00 EDT 2012

NKG2D receptor regulates human effector T-cell cytokine production.

Blood

Barber A,Sentman CL

13-0037 was used in Magnetic cell separation to reveal that human effector T cells alter their function depending on which innate receptors are engaged in conjunction with the TCR complex.

Thu Jun 16 00:00:00 EDT 2011

HIV envelope gp120 activates LFA-1 on CD4 T-lymphocytes and increases cell susceptibility to LFA-1-targeting leukotoxin (LtxA).

PloS one

Hioe CE,Tuen M,Vasiliver-Shamis G,Alvarez Y,Prins KC,Banerjee S,Nádas A,Cho MW,Dustin ML,Kachlany SC

13-0037 was used in Flow cytometry/Cell sorting to evaluate whether cellular activation can be induced by HIV itself, showing that gp120 activates LFA-1 on CD4 T-lymphocytes.

Thu Feb 02 00:00:00 EST 2012

Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

PLoS medicine

Senechal B,Elain G,Jeziorski E,Grondin V,Patey-Mariaud de Serre N,Jaubert F,Beldjord K,Lellouch A,Glorion C,Zerah M,Mary P,Barkaoui M,Emile JF,Boccon-Gibod L,Josset P,Debré M,Fischer A,Donadieu J,Geissmann F

Published figure using CD3 monoclonal antibody (Product # 13-0037-82) in Immunohistochemistry

Wed Aug 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

View more View less

Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

View more View less

UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

View more View less

Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

View more View less

Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD3 Monoclonal Antibody (OKT3), Biotin, eBioscience™

CD3 Antibody (13-0037-82) in Flow

Product Details

13-0037-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Immunomicroscopy (IM)

T-Cell Activation (TCA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!