Invitrogen

CD3 Monoclonal Antibody (OKT3), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

32 Published Figures

27 References

View all (504) CD3 antibodies

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CD3 Antibody (25-0037-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4724-81) (left) or Anti-Human CD3 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone OKT3 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes (middle plot). Additional analysis of lymphocytes shows that CD3 clone OKT3 does not stain any CD19+ B cells (right plot). Details: Normal human whole blood was surface stained with CD3 (clone OKT3) and CD19 (clone HIB19). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used to compare CD3 staining (middle plot). Cells in the lymphocyte gate were used to compare CD3 and CD19 staining (right plot). {RE}

Product Details

25-0037-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT3

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573326

Product Specific Information

Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: This OKT3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Broad and potent neutralizing human antibodies to tick-borne flaviviruses protect mice from disease.

The Journal of experimental medicine

Agudelo M,Palus M,Keeffe JR,Bianchini F,Svoboda P,Salát J,Peace A,Gazumyan A,Cipolla M,Kapoor T,Guidetti F,Yao KH,Elsterová J,Teislerová D,Chrdle A,Hönig V,Oliveira T,West AP,Lee YE,Rice CM,MacDonald MR,Bjorkman PJ,Růžek D,Robbiani DF,Nussenzweig MC

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Mon May 03 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Allergen-specific IgE levels and the ability of IgE-allergen complexes to cross-link determine the extent of CD23-mediated T-cell activation.

The Journal of allergy and clinical immunology

Villazala-Merino S,Rodriguez-Dominguez A,Stanek V,Campion NJ,Gattinger P,Hofer G,Froeschl R,Fae I,Lupinek C,Vrtala S,Breiteneder H,Keller W,Perkmann T,Nakamura R,Pickl WF,Valenta R,Eckl-Dorna J,Niederberger V

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

IL-10 Restores MHC Class I Expression and Interferes With Immunity in Papillary Thyroid Cancer With Hashimoto Thyroiditis.

Endocrinology

Lu ZW,Hu JQ,Liu WL,Wen D,Wei WJ,Wang YL,Wang Y,Liao T,Ji QH

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Small-molecule MMP2/MMP9 inhibitor SB-3CT modulates tumor immune surveillance by regulating PD-L1.

Genome medicine

Ye Y,Kuang X,Xie Z,Liang L,Zhang Z,Zhang Y,Ma F,Gao Q,Chang R,Lee HH,Zhao S,Su J,Li H,Peng J,Chen H,Yin M,Peng C,Yang N,Wang J,Liu J,Liu H,Han L,Chen X

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Thu Feb 06 00:00:00 EST 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Thu Feb 06 00:00:00 EST 2020

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4⁺ T Cell Immunity.

Cell

Binnewies M,Mujal AM,Pollack JL,Combes AJ,Hardison EA,Barry KC,Tsui J,Ruhland MK,Kersten K,Abushawish MA,Spasic M,Giurintano JP,Chan V,Daud AI,Ha P,Ye CJ,Roberts EW,Krummel MF

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Thu Apr 18 00:00:00 EDT 2019

Integrating Ligand-Receptor Interactions and In Vitro Evolution for Streamlined Discovery of Artificial Nucleic Acid Ligands.

Molecular therapy. Nucleic acids

Zumrut HE,Batool S,Argyropoulos KV,Williams N,Azad R,Mallikaratchy PR

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Fri Sep 06 00:00:00 EDT 2019

Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.

Stem cells international

Li C,Huang J,Zhu H,Shi Q,Li D,Ju X

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

MicroRNA‑155 inhibits the proliferation of CD8+ T cells via upregulating regulatory T cells in vitiligo.

Molecular medicine reports

Lv M,Li Z,Liu J,Lin F,Zhang Q,Li Z,Wang Y,Wang K,Xu Y

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy.

Cell reports

Celis-Gutierrez J,Blattmann P,Zhai Y,Jarmuzynski N,Ruminski K,Grégoire C,Ounoughene Y,Fiore F,Aebersold R,Roncagalli R,Gstaiger M,Malissen B

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Cdc42 Couples T Cell Receptor Endocytosis to GRAF1-Mediated Tubular Invaginations of the Plasma Membrane.

Cells

Rossatti P,Ziegler L,Schregle R,Betzler VM,Ecker M,Rossy J

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Mon Nov 04 00:00:00 EST 2019

Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding.

Scientific reports

Jarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Site-specific PEGylation of an anti-CEA/CD3 bispecific antibody improves its antitumor efficacy.

International journal of nanomedicine

Pan H,Liu J,Deng W,Xing J,Li Q,Wang Z

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Mon Jul 02 00:00:00 EDT 2018

Increased ratio of Th17 cells to SIGIRR⁺CD4⁺ T cells in peripheral blood of patients with SLE is associated with disease activity.

Biomedical reports

Xiao JP,Wang DY,Wang XR,Yuan L,Hao L,Wang DG

25-0037 was used in Flow cytometry/Cell sorting to investigate the ratio of T helper cell 17 cells to single immunoglobulin IL-1-related receptor cluster of differentiation T cells in patients with systemic lupus erythematosus.

Mon Oct 01 00:00:00 EDT 2018

NKG2D Ligand-Targeted Bispecific T-Cell Engagers Lead to Robust Antitumor Activity against Diverse Human Tumors.

Molecular cancer therapeutics

Godbersen C,Coupet TA,Huehls AM,Zhang T,Battles MB,Fisher JL,Ernstoff MS,Sentman CL

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Restimulation-induced T-cell death through NTB-A/SAP signaling pathway is impaired in tuberculosis patients with depressed immune responses.

Immunology and cell biology

Hernández Del Pino RE,Pellegrini JM,Rovetta AI,Peña D,Álvarez GI,Rolandelli A,Musella RM,Palmero DJ,Malbran A,Pasquinelli V,García VE

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.

Stem cells international

Chen J,Wang Q,Feng X,Zhang Z,Geng L,Xu T,Wang D,Sun L

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma.

Oncology letters

Liu Z,Han H,He X,Li S,Wu C,Yu C,Wang S

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.

Oncotarget

You Y,Tan JX,Dai HS,Chen HW,Xu XJ,Yang AG,Zhang YJ,Bai LH,Bie P

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Tue Aug 30 00:00:00 EDT 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.

Blood cancer journal

Kim SY,Theunissen JW,Balibalos J,Liao-Chan S,Babcock MC,Wong T,Cairns B,Gonzalez D,van der Horst EH,Perez M,Levashova Z,Chinn L,D'Alessio JA,Flory M,Bermudez A,Jackson DY,Ha E,Monteon J,Bruhns MF,Chen G,Migone TS

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Fri May 29 00:00:00 EDT 2015

Bioengineering T cells to target carbohydrate to treat opportunistic fungal infection.

Proceedings of the National Academy of Sciences of the United States of America

Kumaresan PR,Manuri PR,Albert ND,Maiti S,Singh H,Mi T,Roszik J,Rabinovich B,Olivares S,Krishnamurthy J,Zhang L,Najjar AM,Huls MH,Lee DA,Champlin RE,Kontoyiannis DP,Cooper LJ

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Tue Jul 22 00:00:00 EDT 2014

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.

PloS one

Reichwald K,Jørgensen TZ,Tougaard P,Skov S

Published figure using CD3 monoclonal antibody (Product # 25-0037-42) in Flow Cytometry

Mon Sep 08 00:00:00 EDT 2014

Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions.

PLoS pathogens

Qiu Y,Chen J,Liao H,Zhang Y,Wang H,Li S,Luo Y,Fang D,Li G,Zhou B,Shen L,Chen CY,Huang D,Cai J,Cao K,Jiang L,Zeng G,Chen ZW

25-0037 was used in Flow cytometry/Cell sorting to uncover a previously unknown mechanism for T-cell immune responses regulated by Tim-3, which may have implications for potential immune intervention in TB.

Fri Apr 19 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

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CD3 Monoclonal Antibody (OKT3), PE-Cyanine7, eBioscience™

CD3 Antibody (25-0037-42) in Flow

Product Details

25-0037-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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