Invitrogen

CD25 Monoclonal Antibody (PC61.5), Alexa Fluor™ 700, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

78 Published Figures

88 References

View all (229) CD25 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 80

CD25 Antibody (56-0251-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.125 µg of Rat IgG1 K Isotype Control Alexa Fluor® 700 (Product # 56-4301-80) (left) or 0.125 µg of Anti-Mouse CD25 Alexa Fluor® 700 (right). Total viable cells were used for analysis.

CD25 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD25 Antibody — Staining of stimulated mouse splenocytes. As expected based on known expression patterns, CD25 clone PC61.5 staining is significantly increased in CD8+ T cells after stimulation. Details: Mouse splenocytes were unstimulated (blue histogram) or stimulated for 2 days with CD3 (clone 145-2C11), CD28 (clone 37.51), and Mouse IL-2 Recombinant Protein (purple histogram). After stimulation, cells were stained with CD8 (clone 53-6.7) and CD25 (clone PC61.5). Cells in the CD8+ gate were used for analysis. {TM}

Product Details

56-0251-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 83 publications 83 publications

Neutralization (Neu)

View 2 publications 2 publications

Functional Assay (Functional)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, lambda

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PC61.5

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_891422

Product Specific Information

Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Applications Reported: This PC61.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

6-Mercaptopurine potently inhibits recruitment of SHP2 by phosphorylated PD-1 to inhibit PD-1 signalling and enhance T cell function.

Immunology

Liu L,Lei Y,Zheng Z,Zhou X,Chen S,Zeng G,Yu L,Wang P,Chen L

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2023

Regulatory T-cells and IL-5 mediate pain outcomes in a preclinical model of chronic muscle pain.

Molecular pain

Lenert ME,Szabo-Pardi TA,Burton MD

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2023

Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer's disease.

Nature communications

Choi BJ,Park MH,Park KH,Han WH,Yoon HJ,Jung HY,Hong JY,Chowdhury MR,Kim KY,Lee J,Song IS,Pang M,Choi MK,Gulbins E,Reichel M,Kornhuber J,Hong CW,Kim C,Kim SH,Schuchman EH,Jin HK,Bae JS

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Mar 24 00:00:00 EDT 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Aiolos represses CD4⁺ T cell cytotoxic programming via reciprocal regulation of T_FH transcription factors and IL-2 sensitivity.

Nature communications

Read KA,Jones DM,Pokhrel S,Hales EDS,Varkey A,Tuazon JA,Eisele CD,Abdouni O,Saadey A,Leonard MR,Warren RT,Powell MD,Boss JM,Hemann EA,Yount JS,Xin G,Ghoneim HE,Lio CJ,Freud AG,Collins PL,Oestreich KJ

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Mar 24 00:00:00 EDT 2023

A hydrogen peroxide economizer for on-demand oxygen production-assisted robust sonodynamic immunotherapy.

Theranostics

Jiang Q,Qiao B,Lin X,Cao J,Zhang N,Guo H,Liu W,Zhu L,Xie X,Wan L,Tang R,Liang B,Wang D,Wang Z,Zhou Y,Ran H,Li P

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Mar 07 00:00:00 EST 2022

Glutamine is essential for overcoming the immunosuppressive microenvironment in malignant salivary gland tumors.

Theranostics

Cao S,Hung YW,Wang YC,Chung Y,Qi Y,Ouyang C,Zhong X,Hu W,Coblentz A,Maghami E,Sun Z,Lin HH,Ann DK

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Aug 16 00:00:00 EDT 2022

MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis.

Journal of inflammation research

Liu Y,Zhao N,Xu Q,Deng F,Wang P,Dong L,Lu X,Xia L,Wang M,Chen Z,Zhou J,Zuo D

56-0251-82 was used in Flow cytometry/Cell sorting to suggest that MBL limited the Th17 immunity via controlling the AhR/STAT3 pathway, thus providing new insight into silicosis and other inflammatory diseases in patients with MBL deficiency.

Fri Aug 05 00:00:00 EDT 2022

Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

The Journal of experimental medicine

Hosokawa H,Koizumi M,Masuhara K,Romero-Wolf M,Tanaka T,Nakayama T,Rothenberg EV

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Allergen-Specific Treg Cells Upregulated by Lung-Stage S. japonicum Infection Alleviates Allergic Airway Inflammation.

Frontiers in cell and developmental biology

Li Z,Zhang W,Luo F,Li J,Yang W,Zhu B,Wu Q,Wang X,Sun C,Xie Y,Xu B,Wang Z,Qian F,Chen J,Wan Y,Hu W

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Neutralization

Sat Jun 26 00:00:00 EDT 2021

TRPM8 channel augments T-cell activation and proliferation.

Cell biology international

Acharya TK,Tiwari A,Majhi RK,Goswami C

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo.

Molecular medicine reports

Huo J,Liu T,Li F,Song X,Hou X

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy.

Journal of hematology & oncology

Kim HR,Park JS,Park JH,Yasmin F,Kim CH,Oh SK,Chung IJ,Jun CD

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Positive Effects of Oral Antibiotic Administration in Murine Chronic Graft-Versus-Host Disease.

International journal of molecular sciences

Sato S,Shimizu E,He J,Ogawa M,Asai K,Yazu H,Rusch R,Yamane M,Yang F,Fukuda S,Kawakami Y,Tsubota K,Ogawa Y

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Immunohistochemistry

Sat Apr 03 00:00:00 EDT 2021

Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration.

Cell communication and signaling : CCS

Li J,Wang S,Wang N,Zheng Y,Yang B,Wang X,Zhang J,Pan B,Wang Z

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Aug 30 00:00:00 EDT 2021

The effect of fingolimod on regulatory T cells in a mouse model of brain ischaemia.

Journal of neuroinflammation

Malone K,Diaz Diaz AC,Shearer JA,Moore AC,Waeber C

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sat Jan 30 00:00:00 EST 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Acacetin ameliorates insulin resistance in obesity mice through regulating Treg/Th17 balance via MiR-23b-3p/NEU1 Axis.

BMC endocrine disorders

Wei Y,Jing J,Peng Z,Liu X,Wang X

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Mar 29 00:00:00 EDT 2021

Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

Okada R,Furusawa A,Vermeer DW,Inagaki F,Wakiyama H,Kato T,Nagaya T,Choyke PL,Spanos WC,Allen CT,Kobayashi H

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.

Theranostics

Tong L,Hao H,Zhang Z,Lv Y,Liang X,Liu Q,Liu T,Gong P,Zhang L,Cao F,Pastorin G,Lee CN,Chen X,Wang JW,Yi H

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Jan 28 00:00:00 EST 2022

Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection.

Cell

Ho JSY,Angel M,Ma Y,Sloan E,Wang G,Martinez-Romero C,Alenquer M,Roudko V,Chung L,Zheng S,Chang M,Fstkchyan Y,Clohisey S,Dinan AM,Gibbs J,Gifford R,Shen R,Gu Q,Irigoyen N,Campisi L,Huang C,Zhao N,Jones JD,van Knippenberg I,Zhu Z,Moshkina N,Meyer L,Noel J,Peralta Z,Rezelj V,Kaake R,Rosenberg B,Wang B,Wei J,Paessler S,Wise HM,Johnson J,Vannini A,Amorim MJ,Baillie JK,Miraldi ER,Benner C,Brierley I,Digard P,Łuksza M,Firth AE,Krogan N,Greenbaum BD,MacLeod MK,van Bakel H,Garcìa-Sastre A,Yewdell JW,Hutchinson E,Marazzi I

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2020

Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy.

Cell

Wu L,Hollinshead KER,Hao Y,Au C,Kroehling L,Ng C,Lin WY,Li D,Silva HM,Shin J,Lafaille JJ,Possemato R,Pacold ME,Papagiannakopoulos T,Kimmelman AC,Satija R,Littman DR

56-0251-82 was used in Flow Cytometry to suggest that inhibiting glycolysis by targeting Gpi1 could be an effective therapeutic strategy with minimum toxicity for Th17-mediated autoimmune diseases, and, more generally, that metabolic redundancies can be exploited for selective targeting of disease processes.

Thu Aug 06 00:00:00 EDT 2020

Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity.

The Journal of clinical investigation

Webb LM,Sengupta S,Edell C,Piedra-Quintero ZL,Amici SA,Miranda JN,Bevins M,Kennemer A,Laliotis G,Tsichlis PN,Guerau-de-Arellano M

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages.

EMBO molecular medicine

Menga A,Serra M,Todisco S,Riera-Domingo C,Ammarah U,Ehling M,Palmieri EM,Di Noia MA,Gissi R,Favia M,Pierri CL,Porporato PE,Castegna A,Mazzone M

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Oct 07 00:00:00 EDT 2020

Notch2 complements Notch1 to mediate inductive signaling that initiates early T cell development.

The Journal of cell biology

Romero-Wolf M,Shin B,Zhou W,Koizumi M,Rothenberg EV,Hosokawa H

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Oct 05 00:00:00 EDT 2020

A Small Molecule Inhibitor, OGP46, Is Effective against Imatinib-Resistant BCR-ABL Mutations via the BCR-ABL/JAK-STAT Pathway.

Molecular therapy oncolytics

Wei L,Yang Y,Gupta P,Wang A,Zhao M,Zhao Y,Qu M,Ke Y,Liu Y,Liu HM,Xu X,Sun Y,Chen ZS,Hu Z

56-0251 was used in Flow cytometry/Cell sorting to highlight that OGP46 is active against not only native BCR-ABL but also 11 clinically relevant BCR-ABL mutations, including T315I mutation, which are resistant to imatinib.

Fri Sep 25 00:00:00 EDT 2020

Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.

Journal of cellular and molecular medicine

Wu R,Liu C,Deng X,Chen L,Hao S,Ma L

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Immunoprophylactic and immunotherapeutic control of hormone receptor-positive breast cancer.

Nature communications

Buqué A,Bloy N,Perez-Lanzón M,Iribarren K,Humeau J,Pol JG,Levesque S,Mondragon L,Yamazaki T,Sato A,Aranda F,Durand S,Boissonnas A,Fucikova J,Senovilla L,Enot D,Hensler M,Kremer M,Stoll G,Hu Y,Massa C,Formenti SC,Seliger B,Elemento O,Spisek R,André F,Zitvogel L,Delaloge S,Kroemer G,Galluzzi L

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2020

PTPN2 Deficiency Enhances Programmed T Cell Expansion and Survival Capacity of Activated T Cells.

Cell reports

Flosbach M,Oberle SG,Scherer S,Zecha J,von Hoesslin M,Wiede F,Chennupati V,Cullen JG,List M,Pauling JK,Baumbach J,Kuster B,Tiganis T,Zehn D

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Jul 28 00:00:00 EDT 2020

Artificial Mini Dendritic Cells Boost T Cell-Based Immunotherapy for Ovarian Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Cheng S,Xu C,Jin Y,Li Y,Zhong C,Ma J,Yang J,Zhang N,Li Y,Wang C,Yang Z,Wang Y

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms.

Nature communications

Watanabe M,Lu Y,Breen M,Hodes RJ

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow.

Immunity

Walker JA,Clark PA,Crisp A,Barlow JL,Szeto A,Ferreira ACF,Rana BMJ,Jolin HE,Rodriguez-Rodriguez N,Sivasubramaniam M,Pannell R,Cruickshank J,Daly M,Haim-Vilmovsky L,Teichmann SA,McKenzie ANJ

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Jul 16 00:00:00 EDT 2019

Negative Co-stimulation Constrains T Cell Differentiation by Imposing Boundaries on Possible Cell States.

Immunity

Wei SC,Sharma R,Anang NAS,Levine JH,Zhao Y,Mancuso JJ,Setty M,Sharma P,Wang J,Pe'er D,Allison JP

56-0251 was used in Flow cytometry/Cell sorting to implicate negative co-stimulation as a key regulator and determinant of T cell differentiation and suggest that checkpoint blockade might work in part by altering the limits of T cell phenotypes.

Tue Apr 16 00:00:00 EDT 2019

Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations.

Laboratory investigation; a journal of technical methods and pathology

Hensel JA,Khattar V,Ashton R,Ponnazhagan S

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Interleukin 10 Gene-Modified Bone Marrow-Derived Dendritic Cells Attenuate Liver Fibrosis in Mice by Inducing Regulatory T Cells and Inhibiting the TGF-β/Smad Signaling Pathway.

Mediators of inflammation

Xu Y,Tang X,Yang M,Zhang S,Li S,Chen Y,Liu M,Guo Y,Lu M

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Antitumor and immunomodulatory effects of a novel multitarget inhibitor, CS2164, in mouse hepatocellular carcinoma models.

Anti-cancer drugs

Zhou Y,Fu C,Kong Y,Pan D,Wang Y,Huang S,Li Z,Ning Z,Lu X,Shan S,Xin L

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Sex Differences in Mouse Popliteal Lymph Nodes.

Scientific reports

Dill-Garlow R,Chen KE,Walker AM

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Jan 30 00:00:00 EST 2019

SCGN deficiency results in colitis susceptibility.

eLife

Sifuentes-Dominguez LF,Li H,Llano E,Liu Z,Singla A,Patel AS,Kathania M,Khoury A,Norris N,Rios JJ,Starokadomskyy P,Park JY,Gopal P,Liu Q,Tan S,Chan L,Ross T,Harrison S,Venuprasad K,Baker LA,Jia D,Burstein E

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Oct 30 00:00:00 EDT 2019

Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival.

Scientific reports

Uehara M,Li X,Sheikhi A,Zandi N,Walker B,Saleh B,Banouni N,Jiang L,Ordikhani F,Dai L,Yonar M,Vohra I,Kasinath V,Orgill DP,Khademhosseini A,Annabi N,Abdi R

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Apr 25 00:00:00 EDT 2019

Hippo Kinases Mst1 and Mst2 Sense and Amplify IL-2R-STAT5 Signaling in Regulatory T Cells to Establish Stable Regulatory Activity.

Immunity

Shi H,Liu C,Tan H,Li Y,Nguyen TM,Dhungana Y,Guy C,Vogel P,Neale G,Rankin S,Feng Y,Peng J,Tao W,Chi H

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

Interleukin-17-Producing γδ T Cells Originate from SOX13⁺ Progenitors that Are Independent of γδTCR Signaling.

Immunity

Spidale NA,Sylvia K,Narayan K,Miu B,Frascoli M,Melichar HJ,Zhihao W,Kisielow J,Palin A,Serwold T,Love P,Kobayashi M,Yoshimoto M,Jain N,Kang J

56-0251-82 was used in Flow Cytometry to generate reporter mice for the Tγδ17 lineage-defining transcription factor SOX13 and identify fetal-origin, intrathymic Sox13+ progenitors.

Tue Nov 20 00:00:00 EST 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

56-0251 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 EST 2018

MTOR signaling is essential for the development of thymic epithelial cells and the induction of central immune tolerance.

Autophagy

Liang Z,Zhang L,Su H,Luan R,Na N,Sun L,Zhao Y,Zhang X,Zhang Q,Li J,Zhang L,Zhao Y

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Mar 22 00:00:00 EDT 2019

Granulocyte-macrophage colony-stimulating factor neuroprotective activities in Alzheimer's disease mice.

Journal of neuroimmunology

Kiyota T,Machhi J,Lu Y,Dyavarshetty B,Nemati M,Yokoyama I,Mosley RL,Gendelman HE

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Jun 15 00:00:00 EDT 2018

Immunological effects of a low-dose cytarabine, aclarubicin and granulocyte-colony stimulating factor priming regimen on a mouse leukemia model.

Oncology letters

Chen J,Yang N,Liu H,Yao H,Wang J,Yang Y,Zhang W

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Assessment of tumor growth factor-β1 neutralizing antibody in the treatment of allergic rhinitis and asthma.

Experimental and therapeutic medicine

Wei Y,Zhang Z,Wang F,Zhou S

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment.

eLife

Ng KK,Yui MA,Mehta A,Siu S,Irwin B,Pease S,Hirose S,Elowitz MB,Rothenberg EV,Kueh HY

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Commensal Microbes and Hair Follicle Morphogenesis Coordinately Drive Treg Migration into Neonatal Skin.

Cell host & microbe

Scharschmidt TC,Vasquez KS,Pauli ML,Leitner EG,Chu K,Truong HA,Lowe MM,Sanchez Rodriguez R,Ali N,Laszik ZG,Sonnenburg JL,Millar SE,Rosenblum MD

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2017

Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis.

Cell host & microbe

Zhu F,Willette-Brown J,Song NY,Lomada D,Song Y,Xue L,Gray Z,Zhao Z,Davis SR,Sun Z,Zhang P,Wu X,Zhan Q,Richie ER,Hu Y

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2017

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

EMBO molecular medicine

Huang SC,Wei PC,Hwang-Verslues WW,Kuo WH,Jeng YM,Hu CM,Shew JY,Huang CS,Chang KJ,Lee EY,Lee WH

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Neutralization

Fri Dec 01 00:00:00 EST 2017

Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model.

Oncology letters

Chen J,Zhang M,Zhou F,Wang J,Niu B,Zhang W

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon May 15 00:00:00 EDT 2017

Targeted genome editing restores T cell differentiation in a humanized X-SCID pluripotent stem cell disease model.

Scientific reports

Alzubi J,Pallant C,Mussolino C,Howe SJ,Thrasher AJ,Cathomen T

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Sep 29 00:00:00 EDT 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

Oligodendrocyte death results in immune-mediated CNS demyelination.

Nature neuroscience

Traka M,Podojil JR,McCarthy DP,Miller SD,Popko B

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Autophagy deficiency in myeloid cells increases susceptibility to obesity-induced diabetes and experimental colitis.

Autophagy

Lee HY,Kim J,Quan W,Lee JC,Kim MS,Kim SH,Bae JW,Hur KY,Lee MS

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

Intranasal immunization with heat shock protein 60 induces CD4(+) CD25(+) GARP(+) and type 1 regulatory T cells and inhibits early atherosclerosis.

Clinical and experimental immunology

Zhong Y,Tang H,Wang X,Zeng Q,Liu Y,Zhao XI,Yu K,Shi H,Zhu R,Mao X

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Rapamycin ameliorates CCl4-induced liver fibrosis in mice through reciprocal regulation of the Th17/Treg cell balance.

Molecular medicine reports

Gu L,Deng WS,Sun XF,Zhou H,Xu Q

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model.

Molecular medicine reports

Yue Y,Li P,Song N,Li B,Li Z,Guo Y,Zhang W,Wei MQ,Gai Z,Meng H,Wang J,Qin L

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Chinese herbal medicine formula Gu-Ben-Fang-Xiao-Tang attenuates airway inflammation by modulating Th17/Treg balance in an ovalbumin-induced murine asthma model.

Experimental and therapeutic medicine

Ruan G,Tao B,Wang D,Li Y,Wu J,Yin G

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL.

Biology open

Carofino BL,Ayanga B,Tracey LJ,Brooke-Bisschop T,Justice MJ

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sun May 15 00:00:00 EDT 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Jun 21 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

Immunosuppressive effect of mesenchymal stem cell-derived exosomes on a concanavalin A-induced liver injury model.

Inflammation and regeneration

Tamura R,Uemoto S,Tabata Y

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Mar 31 00:00:00 EDT 2022

Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccination.

Scientific reports

Stifter K,Schuster C,Schlosser M,Boehm BO,Schirmbeck R

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Jul 11 00:00:00 EDT 2016

CCR7 Controls Thymus Recirculation, but Not Production and Emigration, of Foxp3(+) T Cells.

Cell reports

Cowan JE,McCarthy NI,Anderson G

56-0251 was used in Flow cytometry/Cell sorting to investigate CCR7 and Rag2pGFP levels during Treg development, showing that CCR7 controls thymus recirculation, but not production and emigration, of Foxp3(+) T cells.

Tue Feb 09 00:00:00 EST 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Intermuscular and perimuscular fat expansion in obesity correlates with skeletal muscle T cell and macrophage infiltration and insulin resistance.

International journal of obesity (2005)

Khan IM,Perrard XY,Brunner G,Lui H,Sparks LM,Smith SR,Wang X,Shi ZZ,Lewis DE,Wu H,Ballantyne CM

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

A tumor lysate is an effective vaccine antigen for the stimulation of CD4(+) T-cell function and subsequent induction of antitumor immunity mediated by CD8(+) T cells.

Cancer biology & therapy

Kawahara M,Takaku H

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Sep 12 00:00:00 EDT 2016

Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

PloS one

Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2016

High-dose interferon-γ promotes abortion in mice by suppressing Treg and Th17 polarization.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Liu HY,Liu ZK,Chao H,Li Z,Song Z,Yang Y,Peng JP

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2014

Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T-cell differentiation.

Nature communications

Li Q,Zou J,Wang M,Ding X,Chepelev I,Zhou X,Zhao W,Wei G,Cui J,Zhao K,Wang HY,Wang RF

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Dec 22 00:00:00 EST 2014

Reciprocal effects of rab7 deletion in activated and neglected T cells.

Autophagy

Roy SG,Stevens MW,So L,Edinger AL

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

CD40 deficiency in mice exacerbates obesity-induced adipose tissue inflammation, hepatic steatosis, and insulin resistance.

American journal of physiology. Endocrinology and metabolism

Guo CA,Kogan S,Amano SU,Wang M,Dagdeviren S,Friedline RH,Aouadi M,Kim JK,Czech MP

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Nod2 activates NF-kB in CD4+ T cells but its expression is dispensable for T cell-induced colitis.

PloS one

Zanello G,Goethel A,Forster K,Geddes K,Philpott DJ,Croitoru K

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Jul 31 00:00:00 EDT 2014

IL-2 immunotherapy reveals potential for innate beta cell regeneration in the non-obese diabetic mouse model of autoimmune diabetes.

PloS one

Diaz-de-Durana Y,Lau J,Knee D,Filippi C,Londei M,McNamara P,Nasoff M,DiDonato M,Glynne R,Herman AE

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Mon Feb 23 00:00:00 EST 2015

Heat-induced structural changes affect OVA-antigen processing and reduce allergic response in mouse model of food allergy.

PloS one

Golias J,Schwarzer M,Wallner M,Kverka M,Kozakova H,Srutkova D,Klimesova K,Sotkovsky P,Palova-Jelinkova L,Ferreira F,Tuckova L

56-0251 was used in Flow cytometry/Cell sorting to investigate how thermal processing influences the ability of ovalbumin to induce allergic symptoms in a mouse model.

Mon Dec 17 00:00:00 EST 2012

T cell factor 1 regulates thymocyte survival via a RORγt-dependent pathway.

Journal of immunology (Baltimore, Md. : 1950)

Wang R,Xie H,Huang Z,Ma J,Fang X,Ding Y,Sun Z

Published figure using CD25 monoclonal antibody (Product # 56-0251-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Listeria monocytogenes promotes tumor growth via tumor cell toll-like receptor 2 signaling.

Cancer research

Huang B,Zhao J,Shen S,Li H,He KL,Shen GX,Mayer L,Unkeless J,Li D,Yuan Y,Zhang GM,Xiong H,Feng ZH

Tue May 01 00:00:00 EDT 2007

Combination of tumor site-located CTL-associated antigen-4 blockade and systemic regulatory T-cell depletion induces tumor-destructive immune responses.

Cancer research

Tuve S,Chen BM,Liu Y,Cheng TL,Touré P,Sow PS,Feng Q,Kiviat N,Strauss R,Ni S,Li ZY,Roffler SR,Lieber A

Fri Jun 15 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2-RA; IL2R subunit alpha; Il2ra antibody; interleukin-2 receptor alpha; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; p55 chain; sIL 2R; soluble IL 2 receptor

View more View less

Gene Aliases: CD25; Il2r; Il2ra; Ly-43

View more View less

UniProt ID: (Mouse) P01590

View more View less

Entrez Gene ID: (Mouse) 16184

View more View less

Function(s)

interleukin-2 receptor activity protein binding drug binding interleukin-2 binding transmembrane signal receptor

Process(es)

inflammatory response to antigenic stimulus activation-induced cell death of T cells inflammatory response immune response Notch signaling pathway interleukin-2-mediated signaling pathway positive regulation of T cell proliferation positive regulation of activated T cell proliferation negative regulation of T cell proliferation T cell homeostasis positive regulation of T cell differentiation regulation of T cell homeostatic proliferation negative regulation of lymphocyte proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD25 Monoclonal Antibody (PC61.5), Alexa Fluor™ 700, eBioscience™

CD25 Antibody (56-0251-82) in Flow

Product Details

56-0251-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!