Invitrogen

CD45 Monoclonal Antibody (2D1), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

18 Published Figures

34 References

View all (406) CD45 antibodies

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CD45 Antibody (11-9459-42) in Flow

Normal human peripheral blood cells with stained with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD45 FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD45 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

CD45 Antibody — Antibody clone (2D1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in 2D1 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (2D1). Yellow histogram represents staining with the isotype control. {KO}

Product Details

11-9459-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 34 publications 34 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Pig

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2D1

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1907394

Product Specific Information

Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34⁺ hematopoietic stem/progenitor cells.

Annals of translational medicine

Hua J,Jiao T,Qiao Y,Zhang S,Xiao T,Zhang Y,Yan J

11-9459-42 was used in Flow cytometry/Cell sorting to find that HSA was beneficial to maintaining CD34+ cell expansion and short-term colony formation in vitro and optimizing multilineage reconstitution in immunodeficient mice in vivo.

Tue Jan 31 00:00:00 EST 2023

Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo.

Frontiers in immunology

Oyer JL,Croom-Perez TJ,Dieffenthaller TA,Robles-Carillo LD,Gitto SB,Altomare DA,Copik AJ

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2022

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.

International journal of medical sciences

Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2022

Immune composition and its association with hematologic recovery after chemotherapeutic injury in acute myeloid leukemia.

Experimental hematology

Kenswil KJG,Pisterzi P,Feyen J,Ter Borg M,Rombouts E,Braakman E,Raaijmakers MHGP

11-9459-42 was used in Flow cytometry/Cell sorting to provide novel insights into adaptive immune cell recovery after injury and identify the NK/CD4CM index as a putative predictor of hematopoietic recovery in AML.

Sat Jan 01 00:00:00 EST 2022

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.

Bioengineered

Jiang W,Zhang J,Zhang X,Fan C,Huang J

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

11-9459-42 was used in Flow Cytometry to investigate the impact of this Bcl-xL mutant on cartilage differentiation and the expression levels of TGF-β and bone morphogenetic protein (BMP).

Thu Jul 01 00:00:00 EDT 2021

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

11-9459-42 was used in Flow Cytometry to investigate the impact of this Bcl-xL mutant on cartilage differentiation and the expression levels of TGF-β and bone morphogenetic protein (BMP).

Thu Jul 01 00:00:00 EDT 2021

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.

Pathogens & immunity

Bazié WW,Boucher J,Vitry J,Goyer B,Routy JP,Tremblay C,Trottier S,Jenabian MA,Provost P,Alary M,Gilbert C

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

Low-Level Laser Irradiation Promotes Proliferation and Differentiation on Apical Papilla Stem Cells.

Journal of lasers in medical sciences

Gutiérrez D,Rouabhia M,Ortiz J,Gaviria D,Alfonso C,Muñoz A,Inostroza C

11-9459-42 was used in Flow Cytometry to conclude that LLLT promotes proliferation, osteogenic differentiation, and VEGF and TGFβ2 expression on SCAPs.

Fri Feb 18 00:00:00 EST 2022

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Neutralizing Antibodies Protect against Oral Transmission of Lymphocryptovirus.

Cell reports. Medicine

Singh S,Homad LJ,Akins NR,Stoffers CM,Lackhar S,Malhi H,Wan YH,Rawlings DJ,McGuire AT

11-9459 was used in Flow cytometry/Cell sorting to indicate that a vaccine capable of eliciting adequate titers of neutralizing antibodies targeting the AMMO1 epitope may protect against EBV acquisition and are therefore highly relevant to the design of an effective EBV vaccine.

Tue Jun 23 00:00:00 EDT 2020

Elucidating the fundamental fibrotic processes driving abdominal adhesion formation.

Nature communications

Foster DS,Marshall CD,Gulati GS,Chinta MS,Nguyen A,Salhotra A,Jones RE,Burcham A,Lerbs T,Cui L,King ME,Titan AL,Ransom RC,Manjunath A,Hu MS,Blackshear CP,Mascharak S,Moore AL,Norton JA,Kin CJ,Shelton AA,Januszyk M,Gurtner GC,Wernig G,Longaker MT

11-9459 was used in Flow cytometry/Cell sorting to present a systematic analysis of mouse and human adhesion tissues.

Thu Aug 13 00:00:00 EDT 2020

Transcriptomic Profiling of the Tumor Microenvironment Reveals Distinct Subgroups of Clear Cell Renal Cell Cancer: Data from a Randomized Phase III Trial.

Cancer discovery

Hakimi AA,Voss MH,Kuo F,Sanchez A,Liu M,Nixon BG,Vuong L,Ostrovnaya I,Chen YB,Reuter V,Riaz N,Cheng Y,Patel P,Marker M,Reising A,Li MO,Chan TA,Motzer RJ

11-9459 was used in Flow cytometry/Cell sorting to identify predictors of metastatic clear cell RCC (ccRCC) patient response to treatment with tyrosine kinase inhibitor therapy.

Mon Apr 01 00:00:00 EDT 2019

Sodium Selenite Improves The Therapeutic Effect Of BMSCs Via Promoting The Proliferation And Differentiation, Thereby Promoting The Hematopoietic Factors.

OncoTargets and therapy

Yan D,Tang B,Yan L,Zhang L,Miao M,Chen X,Sui G,Zhang Q,Liu D,Wang H

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Tue Apr 12 00:00:00 EDT 2022

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment.

Biology open

Sun Y,Ren Y,Yang F,He Y,Liang S,Guan L,Cheng F,Liu Y,Lin J

11-9459 was used in Flow cytometry/Cell sorting to determine whether primary MenSCs exist in the sedimentation of the deciduous endometrium after DGC and further to evaluate the isolation of MenSCs by direct red blood cell lysis treatment.

Thu May 02 00:00:00 EDT 2019

High-yield isolation of menstrual blood-derived endometrial stem cells by direct red blood cell lysis treatment.

Biology open

Sun Y,Ren Y,Yang F,He Y,Liang S,Guan L,Cheng F,Liu Y,Lin J

Thu May 02 00:00:00 EDT 2019

Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia.

Nature communications

Bell CC,Fennell KA,Chan YC,Rambow F,Yeung MM,Vassiliadis D,Lara L,Yeh P,Martelotto LG,Rogiers A,Kremer BE,Barbash O,Mohammad HP,Johanson TM,Burr ML,Dhar A,Karpinich N,Tian L,Tyler DS,MacPherson L,Shi J,Pinnawala N,Yew Fong C,Papenfuss AT,Grimmond SM,Dawson SJ,Allan RS,Kruger RG,Vakoc CR,Goode DL,Naik SH,Gilan O,Lam EYN,Marine JC,Prinjha RK,Dawson MA

11-9459-42 was used in Flow Cytometry to highlight key principles to help counteract non-genetic drug resistance.

Thu Jun 20 00:00:00 EDT 2019

Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia.

Nature medicine

Zhang B,Nguyen LXT,Li L,Zhao D,Kumar B,Wu H,Lin A,Pellicano F,Hopcroft L,Su YL,Copland M,Holyoake TL,Kuo CJ,Bhatia R,Snyder DS,Ali H,Stein AS,Brewer C,Wang H,McDonald T,Swiderski P,Troadec E,Chen CC,Dorrance A,Pullarkat V,Yuan YC,Perrotti D,Carlesso N,Forman SJ,Kortylewski M,Kuo YH,Marcucci G

11-9459 was used in Flow cytometry/Cell sorting to demonstrate how bone marrow endothelial cells supply miR-126 to leukemia stem cells in chronic myelogenous leukemia which modulates their quiescence and self-renewal.

Tue May 01 00:00:00 EDT 2018

Isolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage.

Molecular medicine reports

Xiao L,Xu S,Wang X,Jin Z,Wang J,Yang B,Xu H

11-9459 was used in Flow cytometry/Cell sorting to show stem cells with multilineage differentiation potential and clonal properties were identified in human LZAC, and these characteristics were more prominent in mildly degenerated as compared with severely degenerated articular cartilage.

Sat Dec 01 00:00:00 EST 2018

BNIP3/Bcl-2-mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate-derived stem cells.

Experimental and therapeutic medicine

Yuan C,Pu L,He Z,Wang J

11-9459-42 was used in Flow Cytometry to investigate the molecular mechanisms of cyclic stretch-induced apoptosis in human intervertebral disc cartilage endplate-derived stem cells (CESCs).

Mon Jan 01 00:00:00 EST 2018

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

A fully defined static suspension culture system for large-scale human embryonic stem cell production.

Cell death & disease

Li X,Ma R,Gu Q,Liang L,Wang L,Zhang Y,Wang X,Liu X,Li Z,Fang J,Wu J,Wang Y,Li W,Hu B,Wang L,Zhou Q,Hao J

11-9459 was used in Flow cytometry/Cell sorting to characterise a static suspension culture system for large-scale human embryonic stem cell production.

Thu Aug 30 00:00:00 EDT 2018

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

Thu Sep 21 00:00:00 EDT 2017

Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance.

Obesity (Silver Spring, Md.)

Subramanian S,Pallati PK,Rai V,Sharma P,Agrawal DK,Nandipati KC

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Region and cell-type resolved quantitative proteomic map of the human heart.

Nature communications

Doll S,Dreßen M,Geyer PE,Itzhak DN,Braun C,Doppler SA,Meier F,Deutsch MA,Lahm H,Lange R,Krane M,Mann M

11-9459 was used in Flow cytometry/Cell sorting to determine the healthy human heart proteome.

Mon Nov 13 00:00:00 EST 2017

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

11-9459 was used in Flow cytometry/Cell sorting to determine NR2F2 plays an important role in the stemness maintenance of bone marrow-derived mesenchymal stem cells.

Tue Jan 19 00:00:00 EST 2016

NR2F2 regulates bone marrow-derived mesenchymal stem cell-promoted proliferation of Reh cells.

Molecular medicine reports

Zhu N,Wang H,Wei J,Wang B,Shan W,Lai X,Zhao Y,Yu J,Huang H

11-9459 was used in Flow cytometry/Cell sorting to demonstrate NR2F2 mediates bone marrow-derived mesenchymal stem cell induced proliferation of Reh cells via regulation of vascular endothelial growth factor A.

Mon Aug 01 00:00:00 EDT 2016

Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures.

Genome biology

Şenbabaoğlu Y,Gejman RS,Winer AG,Liu M,Van Allen EM,de Velasco G,Miao D,Ostrovnaya I,Drill E,Luna A,Weinhold N,Lee W,Manley BJ,Khalil DN,Kaffenberger SD,Chen Y,Danilova L,Voss MH,Coleman JA,Russo P,Reuter VE,Chan TA,Cheng EH,Scheinberg DA,Li MO,Choueiri TK,Hsieh JJ,Sander C,Hakimi AA

11-9459 was used in Flow cytometry/Cell sorting to profile tumour-infiltrating patterns of immune cells using a gene expression-based computational method in 19 human cancers.

Thu Nov 17 00:00:00 EST 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

11-9459 was used in Flow cytometry/Cell sorting to show MIF plays a role in regulating the differentiation fate of human cartilage endplate stem cells under hypoxia.

Tue Aug 09 00:00:00 EDT 2016

Vitamin D machinery and metabolism in porcine adipose-derived mesenchymal stem cells.

Stem cell research & therapy

Valle YL,Almalki SG,Agrawal DK

11-9459 was used in Flow cytometry/Cell sorting to evaluate whether porcine adipose-derived MSCs can metabolise and respond to vitamin D.

Wed Aug 17 00:00:00 EDT 2016

Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.

The Journal of clinical investigation

Gori JL,Butler JM,Chan YY,Chandrasekaran D,Poulos MG,Ginsberg M,Nolan DJ,Elemento O,Wood BL,Adair JE,Rafii S,Kiem HP

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2015

Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.

Stem cell research & therapy

Zhou F,Gao S,Wang L,Sun C,Chen L,Yuan P,Zhao H,Yi Y,Qin Y,Dong Z,Cao L,Ren H,Zhu L,Li Q,Lu B,Liang A,Xu GT,Zhu H,Gao Z,Ma J,Xu J,Chen X

11-9459 was used in Flow cytometry/Cell sorting to demonstrate human adipose derived stem cell (hDAC) intervention with MCAO mice could apparently ameliorate stroke symptoms by direct cell replacement, enhanced immnunosuppression and increasing the viability of endogenous neurons.

Sat May 09 00:00:00 EDT 2015

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD45 monoclonal antibody (Product # 11-9459-42) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45 Monoclonal Antibody (2D1), FITC, eBioscience™

CD45 Antibody (11-9459-42) in Flow

Product Details

11-9459-42

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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