Invitrogen

CD45 Monoclonal Antibody (2D1), APC, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

14 Published Figures

31 References

View all (406) CD45 antibodies

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FIGURE: 1 / 16

CD45 Antibody (17-9459-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD45 APC (purple histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD45 Antibody — Antibody clone (2D1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in 2D1 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (2D1). Yellow histogram represents staining with the isotype control. {KO}

Product Details

17-9459-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 30 publications 30 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2D1

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10718532

Product Specific Information

Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Human serum albumin promotes self-renewal and expansion of umbilical cord blood CD34⁺ hematopoietic stem/progenitor cells.

Annals of translational medicine

Hua J,Jiao T,Qiao Y,Zhang S,Xiao T,Zhang Y,Yan J

17-9459-42 was used in Flow Cytometry /test) to find that HSA was beneficial to maintaining CD34+ cell expansion and short-term colony formation in vitro and optimizing multilineage reconstitution in immunodeficient mice in vivo.

Tue Jan 31 00:00:00 EST 2023

Etiology of end-stage liver cirrhosis impacts hepatic natural killer cell heterogenicity.

Frontiers in immunology

Maretti-Mira AC,Salomon MP,Hsu AM,Dara L,Golden-Mason L

17-9459-42 was used in Flow cytometry/Cell sorting to show that although NK cells shared similar dysfunctions, the disease etiology impacts hepatic NK cell heterogeneity.

Tue Apr 18 00:00:00 EDT 2023

Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo.

Frontiers in immunology

Oyer JL,Croom-Perez TJ,Dieffenthaller TA,Robles-Carillo LD,Gitto SB,Altomare DA,Copik AJ

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2022

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.

International journal of medical sciences

Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2022

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.

Bioengineered

Jiang W,Zhang J,Zhang X,Fan C,Huang J

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.

Pathogens & immunity

Bazié WW,Boucher J,Vitry J,Goyer B,Routy JP,Tremblay C,Trottier S,Jenabian MA,Provost P,Alary M,Gilbert C

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

Matched Targeted Therapy for Pediatric Patients with Relapsed, Refractory, or High-Risk Leukemias: A Report from the LEAP Consortium.

Cancer discovery

Pikman Y,Tasian SK,Sulis ML,Stevenson K,Blonquist TM,Apsel Winger B,Cooper TM,Pauly M,Maloney KW,Burke MJ,Brown PA,Gossai N,McNeer JL,Shukla NN,Cole PD,Kahn JM,Chen J,Barth MJ,Magee JA,Gennarini L,Adhav AA,Clinton CM,Ocasio-Martinez N,Gotti G,Li Y,Lin S,Imamovic A,Tognon CE,Patel T,Faust HL,Contreras CF,Cremer A,Cortopassi WA,Garrido Ruiz D,Jacobson MP,Dharia NV,Su A,Robichaud AL,Saur Conway A,Tarlock K,Stieglitz E,Place AE,Puissant A,Hunger SP,Kim AS,Lindeman NI,Gore L,Janeway KA,Silverman LB,Tyner JW,Harris MH,Loh ML,Stegmaier K

17-9459-42 was used in Flow cytometry/Cell sorting to report the feasibility of identifying targetable mutations in children with leukemia and describe correlative biology studies validating therapeutic hypotheses and novel mutations.

Tue Jun 01 00:00:00 EDT 2021

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

The XPO1 Inhibitor KPT-8602 Synergizes with Dexamethasone in Acute Lymphoblastic Leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research

Verbeke D,Demeyer S,Prieto C,de Bock CE,De Bie J,Gielen O,Jacobs K,Mentens N,Verhoeven BM,Uyttebroeck A,Boeckx N,De Keersmaecker K,Maertens J,Segers H,Cools J

17-9459-42 was used in Flow Cytometry to evaluate whether KPT-8602 would synergize with dexamethasone, vincristine, or doxorubicin, three drugs currently used for the treatment of acute lymphoblastic leukemia.

Sun Nov 01 00:00:00 EDT 2020

The XPO1 Inhibitor KPT-8602 Synergizes with Dexamethasone in Acute Lymphoblastic Leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research

Verbeke D,Demeyer S,Prieto C,de Bock CE,De Bie J,Gielen O,Jacobs K,Mentens N,Verhoeven BM,Uyttebroeck A,Boeckx N,De Keersmaecker K,Maertens J,Segers H,Cools J

Sun Nov 01 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

17-9459 was used in Flow cytometry/Cell sorting to demonstrate miR-221-3p can improve angiogenesis, migration and proliferation of mesenchymal stem cells while suppressing apoptosis.

Mon Sep 28 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

17-9459 was used in Flow cytometry/Cell sorting to demonstrate miR-221-3p can improve angiogenesis, migration and proliferation of mesenchymal stem cells while suppressing apoptosis.

Mon Sep 28 00:00:00 EDT 2020

The IL-6 signaling complex is a critical driver, negative prognostic factor, and therapeutic target in diffuse large B-cell lymphoma.

EMBO molecular medicine

Hashwah H,Bertram K,Stirm K,Stelling A,Wu CT,Kasser S,Manz MG,Theocharides AP,Tzankov A,Müller A

17-9459 was used in Flow cytometry/Cell sorting to show that a subset of diffuse large B-cell lymphoma(DLBCL) cases have evolved mechanisms that ensure constitutive activation of the IL-6 signaling pathway, i.e., the expression of both chains of the IL-6R, the expression of the cytokine itself, and the mutational inactivation of a negative regulator of IL-6 signaling, SOCS1.

Tue Oct 01 00:00:00 EDT 2019

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

A fully defined static suspension culture system for large-scale human embryonic stem cell production.

Cell death & disease

Li X,Ma R,Gu Q,Liang L,Wang L,Zhang Y,Wang X,Liu X,Li Z,Fang J,Wu J,Wang Y,Li W,Hu B,Wang L,Zhou Q,Hao J

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Thu Aug 30 00:00:00 EDT 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

17-9459 was used in Flow cytometry/Cell sorting to demonstrate that blocking PITPNM3 recognition of CCL18 could be an attractive immunotherapy aproach for treating breast cancer.

Sat Apr 01 00:00:00 EDT 2017

Direct Activation of BAX by BTSA1 Overcomes Apoptosis Resistance in Acute Myeloid Leukemia.

Cancer cell

Reyna DE,Garner TP,Lopez A,Kopp F,Choudhary GS,Sridharan A,Narayanagari SR,Mitchell K,Dong B,Bartholdy BA,Walensky LD,Verma A,Steidl U,Gavathiotis E

17-9459 was used in Flow cytometry/Cell sorting to identify BTSA1, a pharmacologically optimised BAX activator that binds with high affinity and specificity to the N-terminal activation site and induces conformational changes to BAX leading to BAX-mediated apoptosis.

Mon Oct 09 00:00:00 EDT 2017

Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance.

Obesity (Silver Spring, Md.)

Subramanian S,Pallati PK,Rai V,Sharma P,Agrawal DK,Nandipati KC

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

A high-yield isolation and enrichment strategy for human lung microvascular endothelial cells.

Pulmonary circulation

Gaskill C,Majka SM

17-9459 was used in Flow cytometry/Cell sorting to describe a protocol to select large quantities of enriched primary human lung microvascular endothelial cells, free from mesenchymal contamination.

Wed Mar 01 00:00:00 EST 2017

High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays.

Nature biotechnology

Cermak N,Olcum S,Delgado FF,Wasserman SC,Payer KR,A Murakami M,Knudsen SM,Kimmerling RJ,Stevens MM,Kikuchi Y,Sandikci A,Ogawa M,Agache V,Baléras F,Weinstock DM,Manalis SR

17-9459 was used in Flow cytometry/Cell sorting to reveal subpopulations of cells with divergent growth kinetics and assess cellular responses to antibiotics and antimicrobial peptides within minutes.

Sat Oct 01 00:00:00 EDT 2016

Chimeric antigen receptor-engineered cytokine-induced killer cells overcome treatment resistance of pre-B-cell acute lymphoblastic leukemia and enhance survival.

International journal of cancer

Oelsner S,Wagner J,Friede ME,Pfirrmann V,Genßler S,Rettinger E,Buchholz CJ,Pfeifer H,Schubert R,Ottmann OG,Ullrich E,Bader P,Wels WS

17-9459 was used in Flow cytometry/Cell sorting to demonstrate potent antileukemic activity of CAR-engineered CIK cells in vitro and in vivo, and suggest this strategy as a promising approach for adoptive immunotherapy of refractory pre-B-ALL.

Sat Oct 15 00:00:00 EDT 2016

Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures.

Genome biology

Şenbabaoğlu Y,Gejman RS,Winer AG,Liu M,Van Allen EM,de Velasco G,Miao D,Ostrovnaya I,Drill E,Luna A,Weinhold N,Lee W,Manley BJ,Khalil DN,Kaffenberger SD,Chen Y,Danilova L,Voss MH,Coleman JA,Russo P,Reuter VE,Chan TA,Cheng EH,Scheinberg DA,Li MO,Choueiri TK,Hsieh JJ,Sander C,Hakimi AA

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Thu Nov 17 00:00:00 EST 2016

A Periodic Diet that Mimics Fasting Promotes Multi-System Regeneration, Enhanced Cognitive Performance, and Healthspan.

Cell metabolism

Brandhorst S,Choi IY,Wei M,Cheng CW,Sedrakyan S,Navarrete G,Dubeau L,Yap LP,Park R,Vinciguerra M,Di Biase S,Mirzaei H,Mirisola MG,Childress P,Ji L,Groshen S,Penna F,Odetti P,Perin L,Conti PS,Ikeno Y,Kennedy BK,Cohen P,Morgan TE,Dorff TB,Longo VD

17-9459 was used in Flow cytometry/Cell sorting to study the effect of prolonged fasting on promoting multi-system regeneration, enhancing cognitive performance, and altering healthspan.

Tue Jul 07 00:00:00 EDT 2015

Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.

The Journal of clinical investigation

Gori JL,Butler JM,Chan YY,Chandrasekaran D,Poulos MG,Ginsberg M,Nolan DJ,Elemento O,Wood BL,Adair JE,Rafii S,Kiem HP

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2015

PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.

Cancer immunology research

Severson JJ,Serracino HS,Mateescu V,Raeburn CD,McIntyre RC,Sams SB,Haugen BR,French JD

17-9459 was used in Flow cytometry/Cell sorting to determine that the release of PD-1 and Tim-3, and the inhibition of Tregs, may encourage tumour elimination in metastatic differentiated thyroid cancer.

Mon Jun 01 00:00:00 EDT 2015

Inhibition of Wild-Type p53-Expressing AML by the Novel Small Molecule HDM2 Inhibitor CGM097.

Molecular cancer therapeutics

Weisberg E,Halilovic E,Cooke VG,Nonami A,Ren T,Sanda T,Simkin I,Yuan J,Antonakos B,Barys L,Ito M,Stone R,Galinsky I,Cowens K,Nelson E,Sattler M,Jeay S,Wuerthner JU,McDonough SM,Wiesmann M,Griffin JD

17-9459 was used in Flow cytometry/Cell sorting to investigate the ability of CGM097 to potently and selectively kill wild type p53 expressing AML cells.

Thu Oct 01 00:00:00 EDT 2015

JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of CML CD34+ cells in vitro and in vivo.

Blood

Gallipoli P,Cook A,Rhodes S,Hopcroft L,Wheadon H,Whetton AD,Jørgensen HG,Bhatia R,Holyoake TL

17-9459 was used in Flow cytometry/Cell sorting to investigate how JAK2/STAT5 inhibition by nilotinib with ruxolitinib contributes to the elimination of CML CD34+ cells in vitro and in vivo.

Thu Aug 28 00:00:00 EDT 2014

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD45 monoclonal antibody (Product # 17-9459-42) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Expression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.

Gastroenterology

Liu S,Li N,Yu X,Xiao X,Cheng K,Hu J,Wang J,Zhang D,Cheng S,Liu S

17-9459 was used in Flow cytometry/Cell sorting to demonstrate that the stem cell factor Nanog regulates expression of ICAM-1 in hepatocellular carcinoma cells.

Wed May 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45 Monoclonal Antibody (2D1), APC, eBioscience™

CD45 Antibody (17-9459-42) in Flow

Product Details

17-9459-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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