Invitrogen

CD45 Monoclonal Antibody (HI30), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

40 Published Figures

51 References

View all (406) CD45 antibodies

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FIGURE: 1 / 42

CD45 Antibody (25-0459-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD45 PE-Cyanine7 (purple histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

Product Details

25-0459-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 50 publications 50 publications

T-Cell Activation (TCA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HI30

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1944375

Product Specific Information

Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Serum amyloid a, C-reactive protein, and procalcitonin levels in children with Mycoplasma pneumoniae infection.

Journal of clinical laboratory analysis

Jiang Y,Wang W,Zhang Z,Ma X,Sang Y,Wang J,Xu G,Feng Q,Zhao S

25-0459-42 was used in T-Cell Activation to show that SAA, CRP, and PCT were specific markers for diagnosing early MP infection in children.

Tue Mar 01 00:00:00 EST 2022

Celecoxib induces adipogenic differentiation of hemangioma-derived mesenchymal stem cells through the PPAR-γ pathway in vitro and in vivo.

Experimental and therapeutic medicine

Wang Y,Kong L,Sun B,Cui J,Shen W

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2022

Blocking STAT3/5 through direct or upstream kinase targeting in leukemic cutaneous T-cell lymphoma.

EMBO molecular medicine

Sorger H,Dey S,Vieyra-Garcia PA,Pölöske D,Teufelberger AR,de Araujo ED,Sedighi A,Graf R,Spiegl B,Lazzeri I,Braun T,Garces de Los Fayos Alonso I,Schlederer M,Timelthaler G,Kodajova P,Pirker C,Surbek M,Machtinger M,Graier T,Perchthaler I,Pan Y,Fink-Puches R,Cerroni L,Ober J,Otte M,Albrecht JD,Tin G,Abdeldayem A,Manaswiyoungkul P,Olaoye OO,Metzelder ML,Orlova A,Berger W,Wobser M,Nicolay JP,André F,Nguyen VA,Neubauer HA,Fleck R,Merkel O,Herling M,Heitzer E,Gunning PT,Kenner L,Moriggl R,Wolf P

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2022

A fluorogenic probe for predicting treatment response in non-small cell lung cancer with EGFR-activating mutations.

Nature communications

Deng H,Lei Q,Wang C,Wang Z,Chen H,Wang G,Yang N,Huang D,Yu Q,Yao M,Xiao X,Zhu G,Cheng C,Li Y,Li F,Tian P,Li W

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Mon Nov 14 00:00:00 EST 2022

Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway.

Experimental and therapeutic medicine

Liu Y,Zhang X,Hu Y,Kang M,Wu Y,Wang Y,Deng C

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

NASH limits anti-tumour surveillance in immunotherapy-treated HCC.

Nature

Pfister D,Núñez NG,Pinyol R,Govaere O,Pinter M,Szydlowska M,Gupta R,Qiu M,Deczkowska A,Weiner A,Müller F,Sinha A,Friebel E,Engleitner T,Lenggenhager D,Moncsek A,Heide D,Stirm K,Kosla J,Kotsiliti E,Leone V,Dudek M,Yousuf S,Inverso D,Singh I,Teijeiro A,Castet F,Montironi C,Haber PK,Tiniakos D,Bedossa P,Cockell S,Younes R,Vacca M,Marra F,Schattenberg JM,Allison M,Bugianesi E,Ratziu V,Pressiani T,D'Alessio A,Personeni N,Rimassa L,Daly AK,Scheiner B,Pomej K,Kirstein MM,Vogel A,Peck-Radosavljevic M,Hucke F,Finkelmeier F,Waidmann O,Trojan J,Schulze K,Wege H,Koch S,Weinmann A,Bueter M,Rössler F,Siebenhüner A,De Dosso S,Mallm JP,Umansky V,Jugold M,Luedde T,Schietinger A,Schirmacher P,Emu B,Augustin HG,Billeter A,Müller-Stich B,Kikuchi H,Duda DG,Kütting F,Waldschmidt DT,Ebert MP,Rahbari N,Mei HE,Schulz AR,Ringelhan M,Malek N,Spahn S,Bitzer M,Ruiz de Galarreta M,Lujambio A,Dufour JF,Marron TU,Kaseb A,Kudo M,Huang YH,Djouder N,Wolter K,Zender L,Marche PN,Decaens T,Pinato DJ,Rad R,Mertens JC,Weber A,Unger K,Meissner F,Roth S,Jilkova ZM,Claassen M,Anstee QM,Amit I,Knolle P,Becher B,Llovet JM,Heikenwalder M

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

An immune-humanized patient-derived xenograft model of estrogen-independent, hormone receptor positive metastatic breast cancer.

Breast cancer research : BCR

Scherer SD,Riggio AI,Haroun F,DeRose YS,Ekiz HA,Fujita M,Toner J,Zhao L,Li Z,Oesterreich S,Samatar AA,Welm AL

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Sat Oct 30 00:00:00 EDT 2021

Direct Comparative Analyses of 10X Genomics Chromium and Smart-seq2.

Genomics, proteomics & bioinformatics

Wang X,He Y,Zhang Q,Ren X,Zhang Z

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Monocyte subsets predict mortality after cardiac arrest.

Journal of leukocyte biology

Krychtiuk KA,Lenz M,Richter B,Hohensinner PJ,Kastl SP,Mangold A,Huber K,Hengstenberg C,Wojta J,Heinz G,Speidl WS

25-0459 was used in Flow cytometry/Cell sorting to suggest a possible role of the innate immune system in the pathophysiology of post cardiac arrest syndrome.

Tue Jun 01 00:00:00 EDT 2021

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

In vitro co-culture of human intestinal organoids and lamina propria-derived CD4⁺ T cells.

STAR protocols

Schreurs RRCE,Baumdick ME,Drewniak A,Bunders MJ

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Longitudinal clonal tracking in humanized mice reveals sustained polyclonal repopulation of gene-modified human-HSPC despite vector integration bias.

Stem cell research & therapy

Suryawanshi GW,Arokium H,Kim S,Khamaikawin W,Lin S,Shimizu S,Chupradit K,Lee Y,Xie Y,Guan X,Suryawanshi V,Presson AP,An DS,Chen ISY

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Thu Oct 07 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.

Nature communications

Asano Y,Daccache J,Jain D,Ko K,Kinloch A,Veselits M,Wolfgeher D,Chang A,Josephson M,Cunningham P,Tambur A,Khan AA,Pillai S,Chong AS,Clark MR

25-0459-42 was used in Flow Cytometry to show a mechanism of human inflammation in which a breach in organ-restricted tolerance by infiltrating innate-like B cells drives local tissue destruction.

Fri Jul 16 00:00:00 EDT 2021

Anti-FLT3 nanoparticles for acute myeloid leukemia: Preclinical pharmacology and pharmacokinetics.

Journal of controlled release : official journal of the Controlled Release Society

Park M,Vaikari VP,Lam AT,Zhang Y,MacKay JA,Alachkar H

25-0459-42 was used in Flow Cytometry to conclude that α-FLT3-A192 fusions appear to be a viable new modality for targeting FLT3 in AML and warrant further preclinical development to bring it into the clinic.

Mon Aug 10 00:00:00 EDT 2020

DKK2 blockage-mediated immunotherapy enhances anti-angiogenic therapy of Kras mutated colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hu J,Wang Z,Chen Z,Li A,Sun J,Zheng M,Wu J,Shen T,Qiao J,Li L,Li B,Wu D,Xiao Q

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Expansion of Group 2 Innate Lymphoid Cells in Patients with End-Stage Renal Disease and Their Clinical Significance.

Journal of immunology (Baltimore, Md. : 1950)

Liu GY,Deng XH,Li X,Cao YJ,Xing YF,Zhou P,Lei AH,Yang Q,Deng K,Zhang H,Zhou J

25-0459-42 was used in Flow Cytometry to indicate that ILC2s may play a protective role in ESRD.

Wed Jul 01 00:00:00 EDT 2020

Evaluation of K18-hACE2 Mice as a Model of SARS-CoV-2 Infection.

The American journal of tropical medicine and hygiene

Moreau GB,Burgess SL,Sturek JM,Donlan AN,Petri WA,Mann BJ

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

The novel reversible LSD1 inhibitor SP-2577 promotes anti-tumor immunity in SWItch/Sucrose-NonFermentable (SWI/SNF) complex mutated ovarian cancer.

PloS one

Soldi R,Ghosh Halder T,Weston A,Thode T,Drenner K,Lewis R,Kaadige MR,Srivastava S,Daniel Ampanattu S,Rodriguez Del Villar R,Lang J,Vankayalapati H,Weissman B,Trent JM,Hendricks WPD,Sharma S

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Thu Sep 10 00:00:00 EDT 2020

In vitro-transcribed antigen receptor mRNA nanocarriers for transient expression in circulating T cells in vivo.

Nature communications

Parayath NN,Stephan SB,Koehne AL,Nelson PS,Stephan MT

25-0459 was used in Flow cytometry/Cell sorting to report an injectable nanocarrier that delivers in vitro-transcribed (IVT) CAR or TCR mRNA for transiently reprograming of circulating T cells to recognize disease-relevant antigens.

Fri Nov 27 00:00:00 EST 2020

In vitro-transcribed antigen receptor mRNA nanocarriers for transient expression in circulating T cells in vivo.

Nature communications

Parayath NN,Stephan SB,Koehne AL,Nelson PS,Stephan MT

Fri Nov 27 00:00:00 EST 2020

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Side-by-side comparison of BH3-mimetics identifies MCL-1 as a key therapeutic target in AML.

Cell death & disease

Ewald L,Dittmann J,Vogler M,Fulda S

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Wed Dec 04 00:00:00 EST 2019

Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.

Cancers

Huang JY,Wang YY,Lo S,Tseng LM,Chen DR,Wu YC,Hou MF,Yuan SF

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Fri Dec 20 00:00:00 EST 2019

Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice.

eLife

Ladinsky MS,Khamaikawin W,Jung Y,Lin S,Lam J,An DS,Bjorkman PJ,Kieffer C

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Mon Oct 28 00:00:00 EDT 2019

Lineage-specific RUNX2 super-enhancer activates MYC and promotes the development of blastic plasmacytoid dendritic cell neoplasm.

Nature communications

Kubota S,Tokunaga K,Umezu T,Yokomizo-Nakano T,Sun Y,Oshima M,Tan KT,Yang H,Kanai A,Iwanaga E,Asou N,Maeda T,Nakagata N,Iwama A,Ohyashiki K,Osato M,Sashida G

25-0459 was used in Flow cytometry/Cell sorting to demonstrate that a plasmacytoid dendritic cell-specific super-enhancer of RUNX2 is associated with the MYC promoter due to t(6;8).

Wed Apr 10 00:00:00 EDT 2019

Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer.

Oncology reports

Wang Y,Guo L,Feng L,Zhang W,Xiao T,Di X,Chen G,Zhang K

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence.

Cell death & disease

Ong SM,Hadadi E,Dang TM,Yeap WH,Tan CT,Ng TP,Larbi A,Wong SC

25-0459 was used in Flow cytometry/Cell sorting to assess three monocyte subsets for evidence of senescence, including proliferative status, telomere length, cellular ROS levels, and mitochondrial membrane potential.

Thu Feb 15 00:00:00 EST 2018

Bacillus anthracis lethal toxin negatively modulates ILC3 function through perturbation of IL-23-mediated MAPK signaling.

PLoS pathogens

Seshadri S,Allan DSJ,Carlyle JR,Zenewicz LA

25-0459 was used in Flow cytometry/Cell sorting to study the effects of MAPK signalling pathways in group 3 innate immune lymphocytes using Bacillus anthracis.

Sun Oct 01 00:00:00 EDT 2017

Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis.

Atherosclerosis

Lebedeva A,Vorobyeva D,Vagida M,Ivanova O,Felker E,Fitzgerald W,Danilova N,Gontarenko V,Shpektor A,Vasilieva E,Margolis L

25-0459 was used in Flow cytometry/Cell sorting to establish a new ex vivo model of human atherosclerotic plaques that preserves the main cell types in plaques and the extracellular components in the context of native cytoarchitecture.

Fri Dec 01 00:00:00 EST 2017

Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection.

Journal of biological research (Thessalonike, Greece)

Apostolou P,Ntanovasilis DA,Papasotiriou I

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.

Oncotarget

Syed Khaja AS,Toor SM,El Salhat H,Faour I,Ul Haq N,Ali BR,Elkord E

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Tue May 16 00:00:00 EDT 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

Azacytidine prevents experimental xenogeneic graft-versus-host disease without abrogating graft-versus-leukemia effects.

Oncoimmunology

Ehx G,Fransolet G,de Leval L,D'Hondt S,Lucas S,Hannon M,Delens L,Dubois S,Drion P,Beguin Y,Humblet-Baron S,Baron F

25-0459 was used in Flow cytometry/Cell sorting to investigate the impact of 5-azacytidine on xenogeneic graft-vs.-host disease and graft-vs.-leukaemia effects in a humanised murine model of transplantation.

Tue Jan 12 00:00:00 EST 2021

Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia.

The Journal of clinical investigation

Jin Y,Zhou J,Xu F,Jin B,Cui L,Wang Y,Du X,Li J,Li P,Ren R,Pan J

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Mon Oct 03 00:00:00 EDT 2016

Microfluidic differential immunocapture biochip for specific leukocyte counting.

Nature protocols

Hassan U,Watkins NN,Reddy B,Damhorst G,Bashir R

25-0459 was used in Flow cytometry/Cell sorting to validate a novel biosensor based on a differential immunocapture technology to enumerate specific cells for diagnostic purposes.

Fri Apr 01 00:00:00 EDT 2016

UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia.

Genes & development

Benyoucef A,Palii CG,Wang C,Porter CJ,Chu A,Dai F,Tremblay V,Rakopoulos P,Singh K,Huang S,Pflumio F,Hébert J,Couture JF,Perkins TJ,Ge K,Dilworth FJ,Brand M

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Platelets confound the measurement of extracellular miRNA in archived plasma.

Scientific reports

Mitchell AJ,Gray WD,Hayek SS,Ko YA,Thomas S,Rooney K,Awad M,Roback JD,Quyyumi A,Searles CD

25-0459 was used in Flow cytometry/Cell sorting to show that a single freeze/thaw cycle of plasma dramatically increases the number of platelet-derived MPs, contaminates the extracellular miRNA pool, and profoundly affects the levels of miRNAs detected.

Tue Sep 13 00:00:00 EDT 2016

Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.

Scientific reports

Liu Z,Guo W,Zhang D,Pang Y,Shi J,Wan S,Cheng K,Wang J,Cheng S

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2016

Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation.

Cell death & disease

Dowling JP,Cai Y,Bertin J,Gough PJ,Zhang J

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2016

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Oncogenic NRAS hyper-activates multiple pathways in human cord blood stem/progenitor cells and promotes myelomonocytic proliferation in vivo.

American journal of translational research

Wang T,Li C,Xia C,Dong Y,Yang D,Geng Y,Cai J,Zhang J,Zhang X,Wang J

25-0459 was used in Flow cytometry/Cell sorting to evaluate the effects of the NRASG12D mutation on human haematopoiesis and leukaemogenesis in vitro and in vivo.

Tue Dec 22 00:00:00 EST 2015

Retinoic acid regulates hematopoietic development from human pluripotent stem cells.

Stem cell reports

Rönn RE,Guibentif C,Moraghebi R,Chaves P,Saxena S,Garcia B,Woods NB

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Tue Feb 10 00:00:00 EST 2015

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Thu Jul 17 00:00:00 EDT 2014

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

Comparative immune phenotypic analysis of cutaneous Squamous Cell Carcinoma and Intraepidermal Carcinoma in immune-competent individuals: proportional representation of CD8+ T-cells but not FoxP3+ Regulatory T-cells is associated with disease stage.

PloS one

Freeman A,Bridge JA,Maruthayanar P,Overgaard NH,Jung JW,Simpson F,Prow TW,Soyer HP,Frazer IH,Freeman M,Wells JW

25-0459 was used in Flow cytometry/Cell sorting to define whether Intraepidermal Carcinoma and Squamous Cell Carcinoma have distinct immune profiles.

Mon Feb 01 00:00:00 EST 2016

Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.

PloS one

Wu S,Ju GQ,Du T,Zhu YJ,Liu GH

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Tue Oct 22 00:00:00 EDT 2013

TTC5 is required to prevent apoptosis of acute myeloid leukemia stem cells.

Cell death & disease

Lynch JT,Somerville TD,Spencer GJ,Huang X,Somervaille TC

25-0459 was used in Flow cytometry/Cell sorting to investigate a model whereby TTC5 and EP300 cooperate to prevent excessive accumulation of MYC in AML cells and their sensitization to cell death.

Thu Apr 04 00:00:00 EDT 2013

ALDH1A isozymes are markers of human melanoma stem cells and potential therapeutic targets.

Stem cells (Dayton, Ohio)

Luo Y,Dallaglio K,Chen Y,Robinson WA,Robinson SE,McCarter MD,Wang J,Gonzalez R,Thompson DC,Norris DA,Roop DR,Vasiliou V,Fujita M

25-0459 was used in Flow cytometry/Cell sorting to demonstrate the existence of melanoma cells that fulfil the criteria for CSCs.

Mon Oct 01 00:00:00 EDT 2012

Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.

Cytotherapy

Yu G,Wu X,Dietrich MA,Polk P,Scott LK,Ptitsyn AA,Gimble JM

Published figure using CD45 monoclonal antibody (Product # 25-0459-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45 Monoclonal Antibody (HI30), PE-Cyanine7, eBioscience™

CD45 Antibody (25-0459-42) in Flow

Product Details

25-0459-42

Flow Cytometry (Flow)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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