Invitrogen

Ki-67 Monoclonal Antibody (SolA15), APC, eBioscience™

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Antibody Testing Data (1)
Published Figures (15)

FIGURE: 1 / 16

Ki-67 Antibody (17-5698-82) in Flow

Intracellular staining of mouse splenocytes unstimulated (left) or stimulated for 2 days with immobilized Anti-Mouse CD3 Functional Grade (Product # 16-0031-82) (right) with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and 0.03 µg of Anti-Mouse/Rat Ki-67 APC using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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Product Details

17-5698-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.06 µg

View 24 publications 24 publications

Product Specifications

Species Reactivity

Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SolA15

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2688057

Product Specific Information

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).

The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.

Applications Reported: This SolA15 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SolA15 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This antibody can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Transformation of primary murine peritoneal mast cells by constitutive KIT activation is accompanied by loss of Cdkn2a/Arf expression.

Frontiers in immunology

Capellmann S,Sonntag R,Schüler H,Meurer SK,Gan L,Kauffmann M,Horn K,Königs-Werner H,Weiskirchen R,Liedtke C,Huber M

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Wed Apr 19 00:00:00 EDT 2023

Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy.

Nature medicine

Ganan-Gomez I,Yang H,Ma F,Montalban-Bravo G,Thongon N,Marchica V,Richard-Carpentier G,Chien K,Manyam G,Wang F,Alfonso A,Chen S,Class C,Kanagal-Shamanna R,Ingram JP,Ogoti Y,Rose A,Loghavi S,Lockyer P,Cambo B,Muftuoglu M,Schneider S,Adema V,McLellan M,Garza J,Marchesini M,Giuliani N,Pellegrini M,Wang J,Walker J,Li Z,Takahashi K,Leverson JD,Bueso-Ramos C,Andreeff M,Clise-Dwyer K,Garcia-Manero G,Colla S

17-5698-82 was used in Flow Cytometry to study the biological mechanisms that drive hypomethylating agent therapy failure at the stem-cell level to uncover vulnerabilities in the disease and halt its evolution.

Tue Mar 01 00:00:00 EST 2022

B-cell antigen receptor expression and phosphatidylinositol 3-kinase signaling regulate genesis and maintenance of mouse chronic lymphocytic leukemia.

Haematologica

Schmid VK,Khadour A,Ahmed N,Brandl C,Nitschke L,Rajewsky K,Jumaa H,Hobeika E

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Prenatal inflammation perturbs murine fetal hematopoietic development and causes persistent changes to postnatal immunity.

Cell reports

López DA,Apostol AC,Lebish EJ,Valencia CH,Romero-Mulero MC,Pavlovich PV,Hernandez GE,Forsberg EC,Cabezas-Wallscheid N,Beaudin AE

17-5698-82 was used in Sample Preparation to show that murine fetal HSPCs respond to prenatal inflammation in utero and that the fetal response shapes postnatal hematopoiesis and immune cell function.

Tue Nov 22 00:00:00 EST 2022

The ectromelia virus virulence factor C15 facilitates early viral spread by inhibiting NK cell contact.

iScience

Peauroi EM,Carro SD,Pei L,Reynoso GV,Hickman HD,Eisenlohr LC

17-5698-82 was used in Flow cytometry/Cell sorting to demonstrate by quantitative immunofluorescence imaging that C15 also limits contact between natural killer (NK) cells and infected cells in vivo.

Thu Dec 22 00:00:00 EST 2022

B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis.

Nature immunology

Schloss MJ,Hulsmans M,Rohde D,Lee IH,Severe N,Foy BH,Pulous FE,Zhang S,Kokkaliaris KD,Frodermann V,Courties G,Yang C,Iwamoto Y,Knudsen AS,McAlpine CS,Yamazoe M,Schmidt SP,Wojtkiewicz GR,Masson GS,Gustafsson K,Capen D,Brown D,Higgins JM,Scadden DT,Libby P,Swirski FK,Naxerova K,Nahrendorf M

17-5698-82 was used in Flow cytometry/Cell sorting to show that B lymphocytes are an important source of acetylcholine, a neurotransmitter of the parasympathetic nervous system, which reduced hematopoiesis.

Fri Apr 01 00:00:00 EDT 2022

CXCR3 regulates stem and proliferative CD8+ T cells during chronic infection by promoting interactions with DCs in splenic bridging channels.

Cell reports

Bangs DJ,Tsitsiklis A,Steier Z,Chan SW,Kaminski J,Streets A,Yosef N,Robey EA

17-5698-82 was used in Immunohistochemistry to observe distinct locations for parasite-specific T cell subsets, implying a link between differentiation and anatomical niches in the spleen.

Tue Jan 18 00:00:00 EST 2022

Uncoupling of macrophage inflammation from self-renewal modulates host recovery from respiratory viral infection.

Immunity

Zhu B,Wu Y,Huang S,Zhang R,Son YM,Li C,Cheon IS,Gao X,Wang M,Chen Y,Zhou X,Nguyen Q,Phan AT,Behl S,Taketo MM,Mack M,Shapiro VS,Zeng H,Ebihara H,Mullon JJ,Edell ES,Reisenauer JS,Demirel N,Kern RM,Chakraborty R,Cui W,Kaplan MH,Zhou X,Goldrath AW,Sun J

17-5698-82 was used in Flow cytometry/Cell sorting to examine the relationship between proliferative and inflammatory properties of tissue macrophages by defining the impact of the Wnt/β-catenin pathway in alveolar macrophages.

Tue Jun 08 00:00:00 EDT 2021

Macrophage LAMTOR1 Deficiency Prevents Dietary Obesity and Insulin Resistance Through Inflammation-Induced Energy Expenditure.

Frontiers in cell and developmental biology

Ying L,Zhang M,Ma X,Si Y,Li X,Su J,Yin J,Bao Y

17-5698-82 was used in Flow Cytometry to study the metabolic function of LAMTOR1 from macrophages using LAMTOR1 macrophage-specific knockout (MKO) mice.

Tue Jun 08 00:00:00 EDT 2021

Macrophage LAMTOR1 Deficiency Prevents Dietary Obesity and Insulin Resistance Through Inflammation-Induced Energy Expenditure.

Frontiers in cell and developmental biology

Ying L,Zhang M,Ma X,Si Y,Li X,Su J,Yin J,Bao Y

17-5698-82 was used in Flow Cytometry to study the metabolic function of LAMTOR1 from macrophages using LAMTOR1 macrophage-specific knockout (MKO) mice.

Tue Jun 08 00:00:00 EDT 2021

HOXA11 plays critical roles in disease progression and response to cytarabine in AML.

Oncology reports

Fu JF,Shih LY,Yen TH

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Mutant ASXL1 induces age-related expansion of phenotypic hematopoietic stem cells through activation of Akt/mTOR pathway.

Nature communications

Fujino T,Goyama S,Sugiura Y,Inoue D,Asada S,Yamasaki S,Matsumoto A,Yamaguchi K,Isobe Y,Tsuchiya A,Shikata S,Sato N,Morinaga H,Fukuyama T,Tanaka Y,Fukushima T,Takeda R,Yamamoto K,Honda H,Nishimura EK,Furukawa Y,Shibata T,Abdel-Wahab O,Suematsu M,Kitamura T

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia.

Cell reports

Suzuki S,McCarrey JR,Hermann BP

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Shikonin Derivatives from Onsoma visianii Decrease Expression of Phosphorylated STAT3 in Leukemia Cells and Exert Antitumor Activity.

Nutrients

Todorovic Z,Milovanovic J,Arsenijevic D,Vukovic N,Vukic M,Arsenijevic A,Djurdjevic P,Milovanovic M,Arsenijevic N

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Wed Mar 31 00:00:00 EDT 2021

XIAP restrains TNF-driven intestinal inflammation and dysbiosis by promoting innate immune responses of Paneth and dendritic cells.

Science immunology

Wahida A,Müller M,Hiergeist A,Popper B,Steiger K,Branca C,Tschurtschenthaler M,Engleitner T,Donakonda S,De Coninck J,Öllinger R,Pfautsch MK,Müller N,Silva M,Usluer S,Thiele Orberg E,Böttcher JP,Pfarr N,Anton M,Slotta-Huspenina JB,Nerlich AG,Madl T,Basic M,Bleich A,Berx G,Ruland J,Knolle PA,Rad R,Adolph TE,Vandenabeele P,Kanegane H,Gessner A,Jost PJ,Yabal M

17-5698-82 was used in Immunohistochemistry to suggest a key role of XIAP in mediating resilience of TLR5-expressing PCs and intestinal DCs, allowing them to maintain tissue integrity and microbiota homeostasis.

Fri Nov 05 00:00:00 EDT 2021

Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1α-NOS2 axis.

Journal of leukocyte biology

Rodrigues TS,Alvarez ARP,Gembre AF,Forni MFPAD,de Melo BMS,Alves Filho JCF,Câmara NOS,Bonato VLD

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Citron Rho-Interacting Serine/Threonine Kinase Promotes HIF1a-CypA Signaling and Growth of Human Pancreatic Adenocarcinoma.

BioMed research international

Cong L,Bai Z,Du Y,Cheng Y

17-5698-82 was used in Flow Cytometry to show that CIT promoted the activation of HIF1-CypA signaling and enhanced the growth of PDAC cells.

Mon Nov 30 00:00:00 EST 2020

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

Kuhn NF,Lopez AV,Li X,Cai W,Daniyan AF,Brentjens RJ

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2020

Treatment of mice with a ligand binding blocking anti-CD28 monoclonal antibody improves healing after myocardial infarction.

PloS one

Gladow N,Hollmann C,Ramos G,Frantz S,Kerkau T,Beyersdorf N,Hofmann U

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.

Nature communications

Riedel R,Addo R,Ferreira-Gomes M,Heinz GA,Heinrich F,Kummer J,Greiff V,Schulz D,Klaeden C,Cornelis R,Menzel U,Kröger S,Stervbo U,Köhler R,Haftmann C,Kühnel S,Lehmann K,Maschmeyer P,McGrath M,Naundorf S,Hahne S,Sercan-Alp Ö,Siracusa F,Stefanowski J,Weber M,Westendorf K,Zimmermann J,Hauser AE,Reddy ST,Durek P,Chang HD,Mashreghi MF,Radbruch A

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Fri May 22 00:00:00 EDT 2020

Stage-Specific Requirement for Eomes in Mature NK Cell Homeostasis and Cytotoxicity.

Cell reports

Wagner JA,Wong P,Schappe T,Berrien-Elliott MM,Cubitt C,Jaeger N,Lee M,Keppel CR,Marin ND,Foltz JA,Marsala L,Neal CC,Sullivan RP,Schneider SE,Keppel MP,Saucier N,Cooper MA,Fehniger TA

17-5698 was used in Flow cytometry/Cell sorting to develop a tamoxifen-inducible, type-1-ILC-specific (Ncr1-targeted) cre mouse and combine this with Eomes-floxed mice.

Tue Jun 02 00:00:00 EDT 2020

TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.

Cell reports

Hurrell BP,Galle-Treger L,Jahani PS,Howard E,Helou DG,Banie H,Soroosh P,Akbari O

17-5698 was used in Flow cytometry/Cell sorting to investigate the role of TNF/TNFR2 axis in pulmonary ILC2s for possible use in treating ILC2-dependent asthma, in murine and human subjects.

Tue Dec 24 00:00:00 EST 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

17-5698 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 EST 2018

De Novo Epigenetic Programs Inhibit PD-1 Blockade-Mediated T Cell Rejuvenation.

Cell

Ghoneim HE,Fan Y,Moustaki A,Abdelsamed HA,Dash P,Dogra P,Carter R,Awad W,Neale G,Thomas PG,Youngblood B

Published figure using Ki-67 monoclonal antibody (Product # 17-5698-82) in Flow Cytometry

Thu Jun 29 00:00:00 EDT 2017

Wnt5a regulates hematopoietic stem cell proliferation and repopulation through the Ryk receptor.

Stem cells (Dayton, Ohio)

Povinelli BJ,Nemeth MJ

17-5698 was used in Flow cytometry to study whether Ryk protein regulates the response of haematopoietic stem cells to Wnt5a.

Wed Jan 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; unnamed protein product

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Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

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UniProt ID: (Human) P46013, (Mouse) E9PVX6

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Entrez Gene ID: (Dog) 100686578, (Human) 4288, (Cynomolgus monkey) 102135895, (Rat) 291234, (Mouse) 17345

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Function(s)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function

Process(es)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ki-67 Monoclonal Antibody (SolA15), APC, eBioscience™

Ki-67 Antibody (17-5698-82) in Flow

Product Details

17-5698-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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