BLOCK-iT™ RNAi Express is a Web tool for finding and ordering Validated Stealth RNAi™ siRNA DuoPaks, Stealth Select RNAi™ siRNA duplexes, BLOCK-iT™ Pol II miR Validated miRNA Vector DuoPaks, and BLOCK-iT™ miR RNAi Select oligos for your genes of interest. Using RNAi Express, you can search for these products by gene symbol, ID number, keyword, or other identifier, or you can browse by biological pathway or gene family.
Note: You can also order In Vivo-purity Stealth RNAi™ and BLOCK-iT™ siRNA Duplexes from custom services, using a separate ordering page.
Validated Stealth RNAi™ siRNA are functionally tested Stealth RNAi™ siRNA duplexes designed for in vitro and in vivo RNA interference (RNAi) analysis of a particular target gene. Each Validated Stealth RNAi™ siRNA duplex is supplied in a ready-to-use format, targets a different region of the gene of interest, and is guaranteed to knock down your gene of interest at greater than 70% efficiency under specified conditions.
Stealth Select RNAi™ siRNA duplexes are predesigned, ready-to-order Stealth sequences for the majority of the selected transcriptome. They have been designed using advance BLAST mismatch search and splice variant coverage criteria, and are supplied in three nonoverlapping sequences per gene so that experiments can be validated using independent sequences. You can order individual duplexes, or a full set of three Stealth Select RNAi™ siRNA that come with a guarantee.
BLOCK-iT™ Pol II miR Validated miRNA Vector DuoPaks are validated expression vectors containing miRNA-based knockdown cassettes driven by RNA Polymerase II (Pol II) promoters for expression in mammalian cells. Each DuoPak includes two qualified, ready-to-use pcDNA™6.2-GW/EmGFP-miR vectors, each with a separate pre-miRNA insert targeting a non-overlapping region of the selected gene of interest. Each validated DuoPak is guaranteed to knock down your gene of interest at greater than 70% efficiency under specified conditions.
BLOCK-iT™ miR RNAi Select oligos are pre-designed, ready-to-anneal-and-clone DNA oligo pairs that result in vector constructs targeting >70% of the human, mouse and rat genes in the RefSeq database. Four different BLOCK-iT™ miR RNAi Select oligo pairs are available per gene, and may be ordered individually or as a set of 4. Each pair is designed to be cloned into a BLOCK-iT™ Pol II miR RNAi Expression Vector (available separately) in a quick and easy procedure. When ordered as a set of four, at least two of the four resulting miR RNAi expression vectors are guaranteed to result in >70% knockdown of the target gene (provided that the transfection efficiency in your experiment is at least 80%).
To begin using BLOCK-iT™ RNAi Express, click on one of the following links next to RNAi Express Options:
Search by ID/Keyword — to search for BLOCK-iT™ RNAi products by gene symbol, accession number, or other identifiers
Browse by Pathways — to identify BLOCK-iT™ RNAi products for key signaling pathways
Browse by Gene Families — to identify BLOCK-iT™ RNAi products by gene family
Note: You must have an account with Invitrogen to order online. In the BLOCK-iT™ RNAi Express order form, you must enter a valid customer number that is keyed to your account. If you do not have an account, you can register online or contact your local representative to set up an account.