Invitrogen

FOXP3 Monoclonal Antibody (PCH101), PE-Cyanine5.5, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

42 Published Figures

46 References

View all (163) FOXP3 antibodies

Datasheet

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Datasheet

Protocols

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FOXP3 Antibody (35-4776-42) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0048-42) followed by intracellular staining with Rat IgG2a K Isotype Control PE-Cyanine5-5 (Product # 35-4321-82) (left) or Anti-Human Foxp3 PE-Cyanine5-5 (right) using the Foxp3/Transcription Factor Staining Buffers (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known relative expression patterns, Foxp3 clone PCH101 stains a subset of the CD4+ T cells and does not stain the CD8+ T cells. Details: Normal human peripheral blood cells were surface stained with CD3 (clone UCHT1), CD4 (clone RPA-T4, top), and CD8 (clone OKT8, bottom), followed by intracellular staining with Rat IgG2a kappa Isotype Control (left) or Foxp3 (clone PCH101, right) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD3+ gate were used for analysis. {RE}

Product Details

35-4776-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 45 publications 45 publications

Product Specifications

Species Reactivity

Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Published species

Human, Mouse, Non-human primate, Virus

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PCH101

Conjugate

PE-Cyanine5.5 PE-Cyanine5.5 PE-Cyanine5.5

Excitation/Emission Max

564/692 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11218682

Product Specific Information

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, Product # 11-0048-42 , or RPA-T4, Product # 11-0049-42 , depending on the species) and CD25 (BC96, Product # 17-0259-42).

Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Please refer to Best Protocols for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In chronic lymphocytic leukemia, activation of the thrombopoietin receptor promotes T-cell inhibitory properties, contributing to immunosuppression.

European journal of haematology

Ringelstein-Harlev S,Fanadka M,Horowitz NA,Bettman NP,Katz T

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Preventive effects of Mycobacterium tuberculosis DNA vaccines on the mouse model with latent tuberculosis infection.

Frontiers in immunology

Liang Y,Li X,Yang Y,Xiao L,Liang Y,Mi J,Xue Y,Gong W,Wang L,Wang J,Zhang J,Shi Y,Peng B,Chen X,Zhao W,Wu X

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2023

AHR/TET2/NT5E axis downregulation is associated with the risk of systemic lupus erythematosus and its progression.

Immunology

Cheng HH,Hung-Ke L,Sheu ML,Lee CY,Tsai YC,Lai DW

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Low-density lipoprotein balances T cell metabolism and enhances response to anti-PD-1 blockade in a HCT116 spheroid model.

Frontiers in oncology

Babl N,Hofbauer J,Matos C,Voll F,Menevse AN,Rechenmacher M,Mair R,Beckhove P,Herr W,Siska PJ,Renner K,Kreutz M,Schnell A

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2023

FABP4 facilitates inflammasome activation to induce the Treg/Th17 imbalance in preeclampsia via forming a positive feedback with IL-17A.

Molecular therapy. Nucleic acids

Chang GP,Yang XL,Liu W,Lin S,Yang SL,Zhao MY

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Soluble fibrinogen‑like protein 2 levels are decreased in patients with ischemic heart failure and associated with cardiac function.

Molecular medicine reports

You Y,Huang S,Liu H,Fan C,Liu K,Wang Z

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Ex vivo expansion of dysfunctional regulatory T lymphocytes restores suppressive function in Parkinson's disease.

NPJ Parkinson's disease

Thome AD,Atassi F,Wang J,Faridar A,Zhao W,Thonhoff JR,Beers DR,Lai EC,Appel SH

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.

Frontiers in immunology

Wang W,Wang X,Lu S,Lv H,Zhao T,Xie G,Du Y,Fan Y,Xu L

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Longitudinal analysis shows durable and broad immune memory after SARS-CoV-2 infection with persisting antibody responses and memory B and T cells.

Cell reports. Medicine

Cohen KW,Linderman SL,Moodie Z,Czartoski J,Lai L,Mantus G,Norwood C,Nyhoff LE,Edara VV,Floyd K,De Rosa SC,Ahmed H,Whaley R,Patel SN,Prigmore B,Lemos MP,Davis CW,Furth S,O'Keefe JB,Gharpure MP,Gunisetty S,Stephens K,Antia R,Zarnitsyna VI,Stephens DS,Edupuganti S,Rouphael N,Anderson EJ,Mehta AK,Wrammert J,Suthar MS,Ahmed R,McElrath MJ

35-4776-42 was used in Flow Cytometry to suggest that broad and effective immunity may persist long-term in recovered COVID-19 patients.

Tue Jul 20 00:00:00 EDT 2021

Longitudinal analysis shows durable and broad immune memory after SARS-CoV-2 infection with persisting antibody responses and memory B and T cells.

medRxiv : the preprint server for health sciences

Cohen KW,Linderman SL,Moodie Z,Czartoski J,Lai L,Mantus G,Norwood C,Nyhoff LE,Edara VV,Floyd K,De Rosa SC,Ahmed H,Whaley R,Patel SN,Prigmore B,Lemos MP,Davis CW,Furth S,O'Keefe J,Gharpure MP,Gunisetty S,Stephens KA,Antia R,Zarnitsyna VI,Stephens DS,Edupuganti S,Rouphael N,Anderson EJ,Mehta AK,Wrammert J,Suthar MS,Ahmed R,McElrath MJ

35-4776-42 was used in Flow Cytometry to evaluate 254 COVID-19 patients longitudinally up to eight months and find durable broad-based immune responses.

Fri Jun 18 00:00:00 EDT 2021

Viable bacterial colonization is highly limited in the human intestine in utero.

Nature medicine

Rackaityte E,Halkias J,Fukui EM,Mendoza VF,Hayzelden C,Crawford ED,Fujimura KE,Burt TD,Lynch SV

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

35-4776 was used in Flow cytometry/Cell sorting to study the HDAC inhibitor CG-745 and its function to enable the synergistic effects of the immune checkpoint inhibitor combination therapy in various cancers by suppressing tumor microenvironment.

Mon Sep 28 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Mon Sep 28 00:00:00 EDT 2020

Plasma Transfusion Promoted Reprogramming CD4⁺ T Lymphocytes Immune Response in Severe Sepsis Mice Model Through Modulating the Exosome Protein Galectin 9.

Cell transplantation

Zhang L,Zhang JP,Liu Y,Wang H,Cheng Y,Wang JH,Zhang WJ,Li ZZ,Guo JR

35-4776 was used in Flow cytometry/Cell sorting to investigate the roles of fresh frozen plasma transfusion in severe sepsis mice model.

Mon Jul 19 00:00:00 EDT 2021

Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal Cancer.

Cell reports. Medicine

Li S,Simoni Y,Becht E,Loh CY,Li N,Lachance D,Koo SL,Lim TP,Tan EKW,Mathew R,Nguyen A,Golovato J,Berkson JD,Prlic M,Lee B,Minot SS,Nagarajan N,Dey N,Tan DSW,Tan IB,Newell EW

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Tue Jun 23 00:00:00 EDT 2020

Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.

JCI insight

Henderson LA,Hoyt KJ,Lee PY,Rao DA,Jonsson AH,Nguyen JP,Rutherford K,Julé AM,Charbonnier LM,Case S,Chang MH,Cohen EM,Dedeoglu F,Fuhlbrigge RC,Halyabar O,Hazen MM,Janssen E,Kim S,Lo J,Lo MS,Meidan E,Son MBF,Sundel RP,Stoll ML,Nusbaum C,Lederer JA,Chatila TA,Nigrovic PA

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Thu Mar 26 00:00:00 EDT 2020

Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations.

Communications biology

Ahl PJ,Hopkins RA,Xiang WW,Au B,Kaliaperumal N,Fairhurst AM,Connolly JE

35-4776 was used in Flow cytometry/Cell sorting to capture the complex metabolic state of any cell as it relates to phenotype and function, leading to a greater understanding of the role of metabolic heterogeneity in immune responses.

Fri Jun 12 00:00:00 EDT 2020

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Double negative T cells mediate Lag3-dependent antigen-specific protection in allergic asthma.

Nature communications

Tian D,Yang L,Wang S,Zhu Y,Shi W,Zhang C,Jin H,Tian Y,Xu H,Sun G,Liu K,Zhang Z,Zhang D

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Wed Sep 18 00:00:00 EDT 2019

Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.

Cancer cell

Arce Vargas F,Furness AJS,Litchfield K,Joshi K,Rosenthal R,Ghorani E,Solomon I,Lesko MH,Ruef N,Roddie C,Henry JY,Spain L,Ben Aissa A,Georgiou A,Wong YNS,Smith M,Strauss D,Hayes A,Nicol D,O'Brien T,Mårtensson L,Ljungars A,Teige I,Frendéus B,Pule M,Marafioti T,Gore M,Larkin J,Turajlic S,Swanton C,Peggs KS,Quezada SA

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Mon Apr 09 00:00:00 EDT 2018

Effect of TGF-β1 on blood CD4⁺CD25^high regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis.

Experimental and therapeutic medicine

Hu Y,Qi W,Sun L,Zhou H,Zhou B,Yang Z

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.

Nature communications

Hsieh WC,Hsu TS,Chang YJ,Lai MZ

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Wed Jan 31 00:00:00 EST 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1.

Journal of virology

Miller SM,Miles B,Guo K,Folkvord J,Meditz AL,McCarter MD,Levy DN,MaWhinney S,Santiago ML,Connick E

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Alteration of Th17 and Foxp3⁺ regulatory T cells in patients with unexplained recurrent spontaneous abortion before and after the therapy of hCG combined with immunoglobulin.

Experimental and therapeutic medicine

Sha J,Liu F,Zhai J,Liu X,Zhang Q,Zhang B

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters.

BMC cancer

McDonnell AM,Cook A,Robinson BWS,Lake RA,Nowak AK

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

ALS patients' regulatory T lymphocytes are dysfunctional, and correlate with disease progression rate and severity.

JCI insight

Beers DR,Zhao W,Wang J,Zhang X,Wen S,Neal D,Thonhoff JR,Alsuliman AS,Shpall EJ,Rezvani K,Appel SH

35-4776 was used in Flow cytometry/Cell sorting to study whether the marked neuroinflammation in ALS can be attributed to the impaired suppressive function of ALS regulatory T lymphocytes in addition to their decreased numbers.

Thu Mar 09 00:00:00 EST 2017

Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.

Cell

Clever D,Roychoudhuri R,Constantinides MG,Askenase MH,Sukumar M,Klebanoff CA,Eil RL,Hickman HD,Yu Z,Pan JH,Palmer DC,Phan AT,Goulding J,Gattinoni L,Goldrath AW,Belkaid Y,Restifo NP

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2016

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.

Journal of immunology (Baltimore, Md. : 1950)

Younis RH,Han KL,Webb TJ

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells.

The American journal of tropical medicine and hygiene

Vieira AP,Trindade MÂ,Pagliari C,Avancini J,Sakai-Valente NY,Duarte AJ,Benard G

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.

Experimental and therapeutic medicine

Zhao J,Ma J,Zhang X,Li Q,Yang X

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants.

Nature immunology

De Rosa V,Galgani M,Porcellini A,Colamatteo A,Santopaolo M,Zuchegna C,Romano A,De Simone S,Procaccini C,La Rocca C,Carrieri PB,Maniscalco GT,Salvetti M,Buscarinu MC,Franzese A,Mozzillo E,La Cava A,Matarese G

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

The role of endogenous IFN-β in the regulation of Th17 responses in patients with relapsing-remitting multiple sclerosis.

Journal of immunology (Baltimore, Md. : 1950)

Tao Y,Zhang X,Chopra M,Kim MJ,Buch KR,Kong D,Jin J,Tang Y,Zhu H,Jewells V,Markovic-Plese S

35-4776 was used in Flow cytometry/Cell sorting to study how in vivo recombinant IFN-β-1a treatment induced IFNAR1 and its downstream signaling molecules' gene expression.

Sun Jun 15 00:00:00 EDT 2014

Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells.

Cellular immunology

Li J,Andreyev O,Chen M,Marco M,Iwase H,Long C,Ayares D,Shen Z,Cooper DK,Ezzelarab MB

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Mon Jan 20 00:00:00 EST 2014

Impaired function of regulatory T cells in cord blood of children of allergic mothers.

Clinical and experimental immunology

Hrdý J,Kocourková I,Prokešová L

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2012

OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Murdoch DM,Staats JS,Weinhold KJ

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.

Investigative ophthalmology & visual science

Dasgupta G,Chentoufi AA,You S,Falatoonzadeh P,Urbano LA,Akhtarmalik A,Nguyen K,Ablabutyan L,Nesburn AB,BenMohamed L

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Tue May 17 00:00:00 EDT 2011

CD40 signalling induces IL-10-producing, tolerogenic dendritic cells.

Experimental dermatology

Tuettenberg A,Fondel S,Steinbrink K,Enk AH,Jonuleit H

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2010

Safety and T cell modulating effects of high dose vitamin D3 supplementation in multiple sclerosis.

PloS one

Smolders J,Peelen E,Thewissen M,Cohen Tervaert JW,Menheere P,Hupperts R,Damoiseaux J

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Mon Dec 13 00:00:00 EST 2010

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2009

IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.

Blood

Ahmadzadeh M,Rosenberg SA

Published figure using FOXP3 monoclonal antibody (Product # 35-4776-42) in Flow Cytometry

Wed Mar 15 00:00:00 EST 2006

Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.

Blood

Epple HJ,Loddenkemper C,Kunkel D,Tröger H,Maul J,Moos V,Berg E,Ullrich R,Schulzke JD,Stein H,Duchmann R,Zeitz M,Schneider T

Wed Nov 01 00:00:00 EST 2006

Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Hillsamer P,Banham AH,Kim CH

Sat Oct 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1, (Cynomolgus monkey) Q6U8D7

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Entrez Gene ID: (Human) 50943, (Cynomolgus monkey) 102120882, (Rhesus monkey) 574303

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FOXP3 Monoclonal Antibody (PCH101), PE-Cyanine5.5, eBioscience™

FOXP3 Antibody (35-4776-42) in Flow

Product Details

35-4776-42

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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