Invitrogen

FOXP3 Monoclonal Antibody (PCH101), Alexa Fluor™ 700, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

57 Published Figures

63 References

View all (163) FOXP3 antibodies

Datasheet

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Datasheet

Protocols

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FOXP3 Antibody (56-4776-41) in Flow

Normal human peripheral blood cells were surface stained with Anti-Human CD4 FITC (Product # 11-0049-42) (left) and Anti-Human CD25 PE (Product # 12-0259-80) (right) then fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and subsequently stained with Anti-Human Foxp3 Alexa Fluor® 700. Cells in the lymphocyte gate were used for analysis.

FOXP3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known relative expression patterns, Foxp3 clone PCH101 stains a subset of the CD4+ T cells and does not stain the CD8+ T cells. Details: Normal human peripheral blood cells were surface stained with CD3 (clone UCHT1), CD4 (clone RPA-T4, top), and CD8 (clone OKT8, bottom), followed by intracellular staining with Rat IgG2a kappa Isotype Control (left) or Foxp3 (clone PCH101, right) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD3+ gate were used for analysis. {RE}

Product Details

56-4776-41

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 12 publications 12 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 50 publications 50 publications

Product Specifications

Species Reactivity

Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PCH101

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582210

Product Specific Information

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, Product # 11-0048-42 , or RPA-T4, Product # 11-0049-42 , depending on the species) and CD25 (BC96, Product # 17-0259-42).

Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set () and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

In chronic lymphocytic leukemia, activation of the thrombopoietin receptor promotes T-cell inhibitory properties, contributing to immunosuppression.

European journal of haematology

Ringelstein-Harlev S,Fanadka M,Horowitz NA,Bettman NP,Katz T

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Preventive effects of Mycobacterium tuberculosis DNA vaccines on the mouse model with latent tuberculosis infection.

Frontiers in immunology

Liang Y,Li X,Yang Y,Xiao L,Liang Y,Mi J,Xue Y,Gong W,Wang L,Wang J,Zhang J,Shi Y,Peng B,Chen X,Zhao W,Wu X

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2023

AHR/TET2/NT5E axis downregulation is associated with the risk of systemic lupus erythematosus and its progression.

Immunology

Cheng HH,Hung-Ke L,Sheu ML,Lee CY,Tsai YC,Lai DW

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Low-density lipoprotein balances T cell metabolism and enhances response to anti-PD-1 blockade in a HCT116 spheroid model.

Frontiers in oncology

Babl N,Hofbauer J,Matos C,Voll F,Menevse AN,Rechenmacher M,Mair R,Beckhove P,Herr W,Siska PJ,Renner K,Kreutz M,Schnell A

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2023

Baricitinib therapy response in rheumatoid arthritis patients associates to STAT1 phosphorylation in monocytes.

Frontiers in immunology

Tucci G,Garufi C,Pacella I,Zagaglioni M,Pinzon Grimaldos A,Ceccarelli F,Conti F,Spinelli FR,Piconese S

56-4776-41 was used in Flow cytometry/Cell sorting to verify the effects of baricitinib on STAT phosphorylation in monocytes of rheumatoid arthritis (RA) patients and evaluate the correlation between STAT phosphorylation and response to therapy.

Mon Aug 15 00:00:00 EDT 2022

Vaccination reshapes the virus-specific T cell repertoire in unexposed adults.

Immunity

Pan YG,Aiamkitsumrit B,Bartolo L,Wang Y,Lavery C,Marc A,Holec PV,Rappazzo CG,Eilola T,Gimotty PA,Hensley SE,Antia R,Zarnitsyna VI,Birnbaum ME,Su LF

56-4776-41 was used in Flow cytometry/Cell sorting to examine how baseline CD4+ T cell repertoire and precursor states impact responses to pathogen infection in humans using primary immunization with yellow fever virus vaccine.

Tue Jun 08 00:00:00 EDT 2021

FABP4 facilitates inflammasome activation to induce the Treg/Th17 imbalance in preeclampsia via forming a positive feedback with IL-17A.

Molecular therapy. Nucleic acids

Chang GP,Yang XL,Liu W,Lin S,Yang SL,Zhao MY

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

VX-765 reduces neuroinflammation after spinal cord injury in mice.

Neural regeneration research

Chen J,Chen YQ,Shi YJ,Ding SQ,Shen L,Wang R,Wang QY,Zha C,Ding H,Hu JG,Lü HZ

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunohistochemistry

Wed Sep 01 00:00:00 EDT 2021

Soluble fibrinogen‑like protein 2 levels are decreased in patients with ischemic heart failure and associated with cardiac function.

Molecular medicine reports

You Y,Huang S,Liu H,Fan C,Liu K,Wang Z

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Ex vivo expansion of dysfunctional regulatory T lymphocytes restores suppressive function in Parkinson's disease.

NPJ Parkinson's disease

Thome AD,Atassi F,Wang J,Faridar A,Zhao W,Thonhoff JR,Beers DR,Lai EC,Appel SH

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.

Frontiers in immunology

Wang W,Wang X,Lu S,Lv H,Zhao T,Xie G,Du Y,Fan Y,Xu L

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Unmasking the immune microecology of ductal carcinoma in situ with deep learning.

NPJ breast cancer

Narayanan PL,Raza SEA,Hall AH,Marks JR,King L,West RB,Hernandez L,Guppy N,Dowsett M,Gusterson B,Maley C,Hwang ES,Yuan Y

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

Viable bacterial colonization is highly limited in the human intestine in utero.

Nature medicine

Rackaityte E,Halkias J,Fukui EM,Mendoza VF,Hayzelden C,Crawford ED,Fujimura KE,Burt TD,Lynch SV

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal Cancer.

Cell reports. Medicine

Li S,Simoni Y,Becht E,Loh CY,Li N,Lachance D,Koo SL,Lim TP,Tan EKW,Mathew R,Nguyen A,Golovato J,Berkson JD,Prlic M,Lee B,Minot SS,Nagarajan N,Dey N,Tan DSW,Tan IB,Newell EW

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Tue Jun 23 00:00:00 EDT 2020

Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.

JCI insight

Henderson LA,Hoyt KJ,Lee PY,Rao DA,Jonsson AH,Nguyen JP,Rutherford K,Julé AM,Charbonnier LM,Case S,Chang MH,Cohen EM,Dedeoglu F,Fuhlbrigge RC,Halyabar O,Hazen MM,Janssen E,Kim S,Lo J,Lo MS,Meidan E,Son MBF,Sundel RP,Stoll ML,Nusbaum C,Lederer JA,Chatila TA,Nigrovic PA

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Thu Mar 26 00:00:00 EDT 2020

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Double negative T cells mediate Lag3-dependent antigen-specific protection in allergic asthma.

Nature communications

Tian D,Yang L,Wang S,Zhu Y,Shi W,Zhang C,Jin H,Tian Y,Xu H,Sun G,Liu K,Zhang Z,Zhang D

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Wed Sep 18 00:00:00 EDT 2019

Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.

JCI insight

Dawson NA,Lamarche C,Hoeppli RE,Bergqvist P,Fung VC,McIver E,Huang Q,Gillies J,Speck M,Orban PC,Bush JW,Mojibian M,Levings MK

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunohistochemistry

Thu Mar 21 00:00:00 EDT 2019

HER2 signaling regulates the tumor immune microenvironment and trastuzumab efficacy.

Oncoimmunology

Triulzi T,Forte L,Regondi V,Di Modica M,Ghirelli C,Carcangiu ML,Sfondrini L,Balsari A,Tagliabue E

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunohistochemistry

Tue Jan 12 00:00:00 EST 2021

Lymphoid Aggregates in the CNS of Progressive Multiple Sclerosis Patients Lack Regulatory T Cells.

Frontiers in immunology

Bell L,Lenhart A,Rosenwald A,Monoranu CM,Berberich-Siebelt F

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunofluorescence

Thu Oct 22 00:00:00 EDT 2020

Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.

Cancer cell

Arce Vargas F,Furness AJS,Litchfield K,Joshi K,Rosenthal R,Ghorani E,Solomon I,Lesko MH,Ruef N,Roddie C,Henry JY,Spain L,Ben Aissa A,Georgiou A,Wong YNS,Smith M,Strauss D,Hayes A,Nicol D,O'Brien T,Mårtensson L,Ljungars A,Teige I,Frendéus B,Pule M,Marafioti T,Gore M,Larkin J,Turajlic S,Swanton C,Peggs KS,Quezada SA

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Mon Apr 09 00:00:00 EDT 2018

Analysis of immunobiologic markers in primary and recurrent glioblastoma.

Journal of neuro-oncology

Rahman M,Kresak J,Yang C,Huang J,Hiser W,Kubilis P,Mitchell D

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunohistochemistry

Sun Apr 01 00:00:00 EDT 2018

Effect of TGF-β1 on blood CD4⁺CD25^high regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis.

Experimental and therapeutic medicine

Hu Y,Qi W,Sun L,Zhou H,Zhou B,Yang Z

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.

Nature communications

Hsieh WC,Hsu TS,Chang YJ,Lai MZ

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Wed Jan 31 00:00:00 EST 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1.

Journal of virology

Miller SM,Miles B,Guo K,Folkvord J,Meditz AL,McCarter MD,Levy DN,MaWhinney S,Santiago ML,Connick E

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Alteration of Th17 and Foxp3⁺ regulatory T cells in patients with unexplained recurrent spontaneous abortion before and after the therapy of hCG combined with immunoglobulin.

Experimental and therapeutic medicine

Sha J,Liu F,Zhai J,Liu X,Zhang Q,Zhang B

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters.

BMC cancer

McDonnell AM,Cook A,Robinson BWS,Lake RA,Nowak AK

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.

Cell

Clever D,Roychoudhuri R,Constantinides MG,Askenase MH,Sukumar M,Klebanoff CA,Eil RL,Hickman HD,Yu Z,Pan JH,Palmer DC,Phan AT,Goulding J,Gattinoni L,Goldrath AW,Belkaid Y,Restifo NP

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2016

CCR6(-) regulatory T cells blunt the restoration of gut Th17 cells along the CCR6-CCL20 axis in treated HIV-1-infected individuals.

Mucosal immunology

Loiseau C,Requena M,Mavigner M,Cazabat M,Carrere N,Suc B,Barange K,Alric L,Marchou B,Massip P,Izopet J,Delobel P

56-4776 was used in Flow cytometry/Cell sorting to study effect of HIV-1 infection on the CCR6-CCL20 chemotactic axis in small intestine epithelial cells.

Thu Sep 01 00:00:00 EDT 2016

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.

Journal of immunology (Baltimore, Md. : 1950)

Younis RH,Han KL,Webb TJ

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells.

The American journal of tropical medicine and hygiene

Vieira AP,Trindade MÂ,Pagliari C,Avancini J,Sakai-Valente NY,Duarte AJ,Benard G

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.

Experimental and therapeutic medicine

Zhao J,Ma J,Zhang X,Li Q,Yang X

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants.

Nature immunology

De Rosa V,Galgani M,Porcellini A,Colamatteo A,Santopaolo M,Zuchegna C,Romano A,De Simone S,Procaccini C,La Rocca C,Carrieri PB,Maniscalco GT,Salvetti M,Buscarinu MC,Franzese A,Mozzillo E,La Cava A,Matarese G

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

Sodium chloride inhibits the suppressive function of FOXP3+ regulatory T cells.

The Journal of clinical investigation

Hernandez AL,Kitz A,Wu C,Lowther DE,Rodriguez DM,Vudattu N,Deng S,Herold KC,Kuchroo VK,Kleinewietfeld M,Hafler DA

56-4776 was used in Flow cytometry/Cell sorting to demonstrate a putative role for an environmental factor that promotes autoimmunity by inducing proinflammatory responses in CD4 effector cells and Treg pathways.

Mon Nov 02 00:00:00 EST 2015

Monitoring regulatory T cells in clinical samples: consensus on an essential marker set and gating strategy for regulatory T cell analysis by flow cytometry.

Cancer immunology, immunotherapy : CII

Santegoets SJ,Dijkgraaf EM,Battaglia A,Beckhove P,Britten CM,Gallimore A,Godkin A,Gouttefangeas C,de Gruijl TD,Koenen HJ,Scheffold A,Shevach EM,Staats J,Taskén K,Whiteside TL,Kroep JR,Welters MJ,van der Burg SH

56-4776 was used in Flow cytometry/Cell sorting to exhibit a novel strategy and system for the context-dependent analysis of Tregs.

Thu Oct 01 00:00:00 EDT 2015

Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults.

Clinical and vaccine immunology : CVI

Boer MC,Prins C,van Meijgaarden KE,van Dissel JT,Ottenhoff TH,Joosten SA

56-4776 was used in Flow cytometry/Cell sorting to discover that BCG vaccination either induced a broad proinflammatory T cell response, or predominantly CD8(+) Treg response.

Wed Jul 01 00:00:00 EDT 2015

Tumor-infiltrating immune cell profiles and their change after neoadjuvant chemotherapy predict response and prognosis of breast cancer.

Breast cancer research : BCR

García-Martínez E,Gil GL,Benito AC,González-Billalabeitia E,Conesa MA,García García T,García-Garre E,Vicente V,Ayala de la Peña F

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunohistochemistry

Sat Nov 29 00:00:00 EST 2014

Highly heterogeneous, activated, and short-lived regulatory T cells during chronic filarial infection.

European journal of immunology

Metenou S,Coulibaly YI,Sturdevant D,Dolo H,Diallo AA,Soumaoro L,Coulibaly ME,Kanakabandi K,Porcella SF,Klion AD,Nutman TB

56-4776 was used in Flow cytometry/Cell sorting to study how in filarial infection, the expanded Treg-cell populations are heterogeneous, short-lived, activated, and express higher levels of molecules known to modulate immune responsiveness.

Tue Jul 01 00:00:00 EDT 2014

CD8+ regulatory T cells, and not CD4+ T cells, dominate suppressive phenotype and function after in vitro live Mycobacterium bovis-BCG activation of human cells.

PloS one

Boer MC,van Meijgaarden KE,Joosten SA,Ottenhoff TH

56-4776 was used in Flow cytometry/Cell sorting to analyse the suppressive phenotype and function of CD4+ versus CD8+ T cells after in vitro live bacillus Calmette-Guerin activation of human cells.

Wed Dec 03 00:00:00 EST 2014

Human Treg responses allow sustained recombinant adeno-associated virus-mediated transgene expression.

The Journal of clinical investigation

Mueller C,Chulay JD,Trapnell BC,Humphries M,Carey B,Sandhaus RA,McElvaney NG,Messina L,Tang Q,Rouhani FN,Campbell-Thompson M,Fu AD,Yachnis A,Knop DR,Ye GJ,Brantly M,Calcedo R,Somanathan S,Richman LP,Vonderheide RH,Hulme MA,Brusko TM,Wilson JM,Flotte TR

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunofluorescence

Sun Dec 01 00:00:00 EST 2013

T regulatory cells and related immunoregulatory factors in polymorphic light eruption following ultraviolet A1 challenge.

The British journal of dermatology

Gambichler T,Terras S,Kampilafkos P,Kreuter A,Skrygan M

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunofluorescence

Sun Dec 01 00:00:00 EST 2013

Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells.

Cellular immunology

Li J,Andreyev O,Chen M,Marco M,Iwase H,Long C,Ayares D,Shen Z,Cooper DK,Ezzelarab MB

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Mon Jan 20 00:00:00 EST 2014

Induction of endometriosis alters the peripheral and endometrial regulatory T cell population in the non-human primate.

Human reproduction (Oxford, England)

Braundmeier A,Jackson K,Hastings J,Koehler J,Nowak R,Fazleabas A

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunohistochemistry

Fri Jun 01 00:00:00 EDT 2012

Impaired function of regulatory T cells in cord blood of children of allergic mothers.

Clinical and experimental immunology

Hrdý J,Kocourková I,Prokešová L

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2012

The vaccine-site microenvironment induced by injection of incomplete Freund's adjuvant, with or without melanoma peptides.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Harris RC,Chianese-Bullock KA,Petroni GR,Schaefer JT,Brill LB,Molhoek KR,Deacon DH,Patterson JW,Slingluff CL

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunohistochemistry

Sun Jan 01 00:00:00 EST 2012

OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Murdoch DM,Staats JS,Weinhold KJ

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Increased expression of regulatory T cells and down-regulatory molecules in lepromatous leprosy.

The American journal of tropical medicine and hygiene

Palermo ML,Pagliari C,Trindade MA,Yamashitafuji TM,Duarte AJ,Cacere CR,Benard G

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunohistochemistry

Tue May 01 00:00:00 EDT 2012

Increased frequency of regulatory T cells accompanies increased immune activation in rectal mucosae of HIV-positive noncontrollers.

Journal of virology

Shaw JM,Hunt PW,Critchfield JW,McConnell DH,Garcia JC,Pollard RB,Somsouk M,Deeks SG,Shacklett BL

56-4776 was used in Flow cytometry/Cell sorting to investigate the role of Tregs in persistent HIV infection, showing that an increased frequency of Tregs accompanies increased immune activation in HIV-positive noncontrollers.

Tue Nov 01 00:00:00 EDT 2011

Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.

Investigative ophthalmology & visual science

Dasgupta G,Chentoufi AA,You S,Falatoonzadeh P,Urbano LA,Akhtarmalik A,Nguyen K,Ablabutyan L,Nesburn AB,BenMohamed L

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Tue May 17 00:00:00 EDT 2011

CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.

The Journal of infectious diseases

Green AM,Mattila JT,Bigbee CL,Bongers KS,Lin PL,Flynn JL

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Immunofluorescence

Sun Aug 15 00:00:00 EDT 2010

CD40 signalling induces IL-10-producing, tolerogenic dendritic cells.

Experimental dermatology

Tuettenberg A,Fondel S,Steinbrink K,Enk AH,Jonuleit H

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2010

Safety and T cell modulating effects of high dose vitamin D3 supplementation in multiple sclerosis.

PloS one

Smolders J,Peelen E,Thewissen M,Cohen Tervaert JW,Menheere P,Hupperts R,Damoiseaux J

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Mon Dec 13 00:00:00 EST 2010

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

Published figure using FOXP3 monoclonal antibody (Product # 56-4776-41) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2009

De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.

Journal of immunology (Baltimore, Md. : 1950)

Wang J,Huizinga TW,Toes RE

56-4776 was used in Flow cytometry/Cell sorting to investigate methods to convert T cells into Treg cells, showing that retinoic acid can be used for de novo generation of Tregs.

Tue Sep 15 00:00:00 EDT 2009

IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.

Blood

Ahmadzadeh M,Rosenberg SA

Wed Mar 15 00:00:00 EST 2006

Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.

Blood

Epple HJ,Loddenkemper C,Kunkel D,Tröger H,Maul J,Moos V,Berg E,Ullrich R,Schulzke JD,Stein H,Duchmann R,Zeitz M,Schneider T

Wed Nov 01 00:00:00 EST 2006

Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Hillsamer P,Banham AH,Kim CH

Sat Oct 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1, (Cynomolgus monkey) Q6U8D7

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Entrez Gene ID: (Human) 50943, (Cynomolgus monkey) 102120882, (Rhesus monkey) 574303

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding

Process(es)

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FOXP3 Monoclonal Antibody (PCH101), Alexa Fluor™ 700, eBioscience™

FOXP3 Antibody (56-4776-41) in Flow

Product Details

56-4776-41

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

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