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CD45 Monoclonal Antibody (HI30), PE-Cyanine7

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2 Published Figures

8 References

View all (406) CD45 antibodies

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FIGURE: 1 / 4

CD45 Antibody (MHCD4512) in Flow

Ammonium chloride lysed whole blood was stained with mouse anti human CD45 PE-Cy7 (Product # MHCD4512), washed, and analyzed on a flow cytometer with a 488nm excitation source.

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

Product Details

MHCD4512

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Dog, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HI30

Immunogen

CD45 antigen

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA, sucrose

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372215

Product Specific Information

The R-phycoerythrin (PE)-Cy7® tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a 488 nm laser without occupying traditional channels associated with FITC and PE. Alternatively, the PE dye tandem can be excited using a 532 nm or 561 nm laser.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Analyte Specific Reagent

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Product References

Transcriptional, epigenetic and metabolic signatures in cardiometabolic syndrome defined by extreme phenotypes.

Clinical epigenetics

Seyres D,Cabassi A,Lambourne JJ,Burden F,Farrow S,McKinney H,Batista J,Kempster C,Pietzner M,Slingsby O,Cao TH,Quinn PA,Stefanucci L,Sims MC,Rehnstrom K,Adams CL,Frary A,Ergüener B,Kreuzhuber R,Mocciaro G,D'Amore S,Koulman A,Grassi L,Griffin JL,Ng LL,Park A,Savage DB,Langenberg C,Bock C,Downes K,Wareham NJ,Allison M,Vacca M,Kirk PDW,Frontini M

MHCD4512 was used in Flow Cytometry to investigate cardiometabolic syndrome pathophysiology and its relationship with thrombosis by generating a multi-omic disease signature.

Sat Mar 12 00:00:00 EST 2022

Transcriptional, epigenetic and metabolic signatures in cardiometabolic syndrome defined by extreme phenotypes.

Clinical epigenetics

MHCD4512 was used in Flow Cytometry to investigate cardiometabolic syndrome pathophysiology and its relationship with thrombosis by generating a multi-omic disease signature.

Sat Mar 12 00:00:00 EST 2022

Mesenchymal stem cells negatively regulate CD4⁺ T cell activation in patients with primary Sjögren syndrome through the miRNA‑125b and miRNA‑155 TCR pathway.

Molecular medicine reports

Gong B,Zheng L,Lu Z,Huang J,Pu J,Pan S,Zhang M,Liu J,Tang J

MHCD4512 was used in Flow cytometry/Cell sorting to indicate that the expression levels of miRNA‚125b‚5p and miRNA‚155 in TCR‚activated CD4+ T cells from patients with pSS may provide insight regarding autoimmune diseases and offer a novel target for prospective treatment.

Fri Jan 01 00:00:00 EST 2021

miR-9 Does Not Regulate Lamin A Expression in Metastatic Cells from Lung Adenocarcinoma.

International journal of molecular sciences

Guinde J,Benoit A,Frankel D,Robert S,Ostacolo K,Lévy N,Astoul P,Roll P,Kaspi E

MHCD4512 was used in Flow Cytometry to explore the potential of miR-9 as candidate to target for lung adenocarcinoma.

Wed Feb 26 00:00:00 EST 2020

miR-9 Does Not Regulate Lamin A Expression in Metastatic Cells from Lung Adenocarcinoma.

International journal of molecular sciences

Guinde J,Benoit A,Frankel D,Robert S,Ostacolo K,Lévy N,Astoul P,Roll P,Kaspi E

MHCD4512 was used in Flow Cytometry to explore the potential of miR-9 as candidate to target for lung adenocarcinoma.

Wed Feb 26 00:00:00 EST 2020

Elucidating the fundamental fibrotic processes driving abdominal adhesion formation.

Nature communications

Foster DS,Marshall CD,Gulati GS,Chinta MS,Nguyen A,Salhotra A,Jones RE,Burcham A,Lerbs T,Cui L,King ME,Titan AL,Ransom RC,Manjunath A,Hu MS,Blackshear CP,Mascharak S,Moore AL,Norton JA,Kin CJ,Shelton AA,Januszyk M,Gurtner GC,Wernig G,Longaker MT

MHCD4512 was used in Flow cytometry/Cell sorting to present a systematic analysis of mouse and human adhesion tissues.

Thu Aug 13 00:00:00 EDT 2020

Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status.

PloS one

Kaspi E,Frankel D,Guinde J,Perrin S,Laroumagne S,Robaglia-Schlupp A,Ostacolo K,Harhouri K,Tazi-Mezalek R,Micallef J,Dutau H,Tomasini P,De Sandre-Giovannoli A,Lévy N,Cau P,Astoul P,Roll P

MHCD4512 was used in Flow cytometry/Cell sorting to investigate lamins in cancer cells from metastatic pleural effusions.

Mon Oct 09 00:00:00 EDT 2017

Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status.

PloS one

Kaspi E,Frankel D,Guinde J,Perrin S,Laroumagne S,Robaglia-Schlupp A,Ostacolo K,Harhouri K,Tazi-Mezalek R,Micallef J,Dutau H,Tomasini P,De Sandre-Giovannoli A,Lévy N,Cau P,Astoul P,Roll P

MHCD4512 was used in Flow cytometry/Cell sorting to investigate lamins in cancer cells from metastatic pleural effusions.

Mon Oct 09 00:00:00 EDT 2017

Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.

Mucosal immunology

Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M

MHCD4512 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.

Fri Jan 01 00:00:00 EST 2016

Increased DNA methylation variability in type 1 diabetes across three immune effector cell types.

Nature communications

Paul DS,Teschendorff AE,Dang MA,Lowe R,Hawa MI,Ecker S,Beyan H,Cunningham S,Fouts AR,Ramelius A,Burden F,Farrow S,Rowlston S,Rehnstrom K,Frontini M,Downes K,Busche S,Cheung WA,Ge B,Simon MM,Bujold D,Kwan T,Bourque G,Datta A,Lowy E,Clarke L,Flicek P,Libertini E,Heath S,Gut M,Gut IG,Ouwehand WH,Pastinen T,Soranzo N,Hofer SE,Karges B,Meissner T,Boehm BO,Cilio C,Elding Larsson H,Lernmark Å,Steck AK,Rakyan VK,Beck S,Leslie RD

MHCD4512 was used in flow cytometry to discuss the contribution of epigenetic changes to the development of type 1 diabetes

Tue Nov 29 00:00:00 EST 2016

Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.

Methods in molecular biology (Clifton, N.J.)

Dang MN,Bradford CM,Pozzilli P,Leslie RD

MHCD4512 was used in flow cytometry to discuss cell- and tissue-specific changes in DNA methylation in type 1 diabetes

Fri Oct 20 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45 Monoclonal Antibody (HI30), PE-Cyanine7

CD45 Antibody (MHCD4512) in Flow

Product Details

MHCD4512

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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