Invitrogen

CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 488

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

11 Published Figures

17 References

View all (406) CD45 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

CD45 Antibody (MHCD4520) in ICC/IF

Theranostics 2021 - Figure 4 Benchmarking ALICE CTC phenotypic count against human enumeration in real fluorescent images. Identification, localization and enumeration of CTC phenotypes: (A) HE4- (DAPI+/CD45-/E&M+/HE4-) and HE4+ (DAPI+/CD45-/E&M+/HE4+) CTCs from 61 ovarian cancer patients, (B) E CTCs (DAPI+/CD45-/E-cadherin+/vimentin-), H CTCs (DAPI+/CD45-/E-cadherin+/vimentin+) and M CTCs (DAPI+/CD45-/E-cadherin-/vimentin+) f... View More

CD45 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD45 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

Product Details

MHCD4520

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Flow Cytometry (Flow)

Assay-Dependent

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HI30

Immunogen

CD45 antigen

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10392555

Product Specific Information

Based on our testing, publications, and results reported from customers, the Alexa Fluor® 488 dye provides the best fluorescein (FITC) substitute.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Analyte Specific Reagent

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Product References

HiCAR is a robust and sensitive method to analyze open-chromatin-associated genome organization.

Molecular cell

Wei X,Xiang Y,Peters DT,Marius C,Sun T,Shan R,Ou J,Lin X,Yue F,Li W,Southerland KW,Diao Y

MHCD4520 was used in Flow cytometry/Cell sorting to find that the poised" gene promoters exhibit silencer-like function to repress the expression of distal genes via promoter-promoter interactions."

Thu Mar 17 00:00:00 EDT 2022

Fasting induces a highly resilient deep quiescent state in muscle stem cells via ketone body signaling.

Cell metabolism

Benjamin DI,Both P,Benjamin JS,Nutter CW,Tan JH,Kang J,Machado LA,Klein JDD,de Morree A,Kim S,Liu L,Dulay H,Feraboli L,Louie SM,Nomura DK,Rando TA

MHCD4520 was used in Flow cytometry/Cell sorting to report that fasting slows muscle repair both immediately after the conclusion of fasting as well as after multiple days of refeeding.

Tue Jun 07 00:00:00 EDT 2022

Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting.

Nature biomedical engineering

Labib M,Wang Z,Ahmed SU,Mohamadi RM,Duong B,Green B,Sargent EH,Kelley SO

MHCD4520 was used in Immunocytochemistry to show that mutated proteins that are drug targets or markers of therapeutic response can be directly identified in CTCs, analysed at the single-cell level and used to predict how mice with drug-susceptible and drug-resistant pancreatic tumour xenografts respond to therapy.

Fri Jan 01 00:00:00 EST 2021

ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells.

Theranostics

Cheng KS,Pan R,Pan H,Li B,Meena SS,Xing H,Ng YJ,Qin K,Liao X,Kosgei BK,Wang Z,Han RPS

MHCD4520 was used in Immunocytochemistry to present a machine-learning-augmented rule-based hybrid AI algorithm with enhanced cybersecurity and connectivity for the automatic and flexibly-adapting enumeration of cellular liquid biopsies.

Mon May 31 00:00:00 EDT 2021

ALICE: a hybrid AI paradigm with enhanced connectivity and cybersecurity for a serendipitous encounter with circulating hybrid cells.

Theranostics

Cheng KS,Pan R,Pan H,Li B,Meena SS,Xing H,Ng YJ,Qin K,Liao X,Kosgei BK,Wang Z,Han RPS

Mon May 31 00:00:00 EDT 2021

Transient non-integrative expression of nuclear reprogramming factors promotes multifaceted amelioration of aging in human cells.

Nature communications

Sarkar TJ,Quarta M,Mukherjee S,Colville A,Paine P,Doan L,Tran CM,Chu CR,Horvath S,Qi LS,Bhutani N,Rando TA,Sebastiano V

MHCD4520 was used in Flow Cytometry to show that transient expression of nuclear reprogramming factors, mediated by expression of mRNAs, promotes a rapid and broad amelioration of cellular aging.

Tue Mar 24 00:00:00 EDT 2020

Mitochondrial Transfer of Wharton's Jelly Mesenchymal Stem Cells Eliminates Mutation Burden and Rescues Mitochondrial Bioenergetics in Rotenone-Stressed MELAS Fibroblasts.

Oxidative medicine and cellular longevity

Lin TK,Chen SD,Chuang YC,Lan MY,Chuang JH,Wang PW,Hsu TY,Wang FS,Tsai MH,Huang ST,Wang XW,Tsai PC,Lin HY,Liou CW

MHCD4520 was used in Flow cytometry/Cell sorting to determine the therapeutic effect of Wharton's jelly mesenchymal stem cells on mitochondiral dysfunction in MELAS patients.

Fri Dec 13 00:00:00 EST 2019

CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients.

EBioMedicine

Sun Y,Wu G,Cheng KS,Chen A,Neoh KH,Chen S,Tang Z,Lee PF,Dai M,Han RPS

MHCD4520 was used in Flow cytometry to report on the clinical value of a phenotypic circulating tumor cell (CTC)-based blood test for a preoperative prognostic assessment of tumor metastasis and overall survival (OS) of PDAC patients.

Thu Aug 01 00:00:00 EDT 2019

CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients.

EBioMedicine

Sun Y,Wu G,Cheng KS,Chen A,Neoh KH,Chen S,Tang Z,Lee PF,Dai M,Han RPS

Thu Aug 01 00:00:00 EDT 2019

CTC phenotyping for a preoperative assessment of tumor metastasis and overall survival of pancreatic ductal adenocarcinoma patients.

EBioMedicine

Sun Y,Wu G,Cheng KS,Chen A,Neoh KH,Chen S,Tang Z,Lee PF,Dai M,Han RPS

Thu Aug 01 00:00:00 EDT 2019

Single-cell mRNA cytometry via sequence-specific nanoparticle clustering and trapping.

Nature chemistry

Labib M,Mohamadi RM,Poudineh M,Ahmed SU,Ivanov I,Huang CL,Moosavi M,Sargent EH,Kelley SO

MHCD4520 was used in Immunocytochemistry-immunoflourescence to develop a method for cell analysis-single-cell mRNA cytometry-that enables the isolation of rare cells from whole blood as a function of target mRNA sequences.

Tue May 01 00:00:00 EDT 2018

A patient derived xenograft model of cervical cancer and cervical dysplasia.

PloS one

Larmour LI,Cousins FL,Teague JA,Deane JA,Jobling TW,Gargett CE

Published figure using CD45 monoclonal antibody (Product # MHCD4520) in Immunohistochemistry

Fri Mar 29 00:00:00 EDT 2019

Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss.

Nature communications

Quarta M,Cromie M,Chacon R,Blonigan J,Garcia V,Akimenko I,Hamer M,Paine P,Stok M,Shrager JB,Rando TA

Published figure using CD45 monoclonal antibody (Product # MHCD4520) in Flow Cytometry

Tue Jun 20 00:00:00 EDT 2017

Tracking the dynamics of circulating tumour cell phenotypes using nanoparticle-mediated magnetic ranking.

Nature nanotechnology

Poudineh M,Aldridge PM,Ahmed S,Green BJ,Kermanshah L,Nguyen V,Tu C,Mohamadi RM,Nam RK,Hansen A,Sridhar SS,Finelli A,Fleshner NE,Joshua AM,Sargent EH,Kelley SO

MHCD4520 was used in flow cytometry to investigate use of nanoparticle-mediated magnetic ranking for heterogeneous circulating tumour cells.

Wed Mar 01 00:00:00 EST 2017

An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy.

Nature biotechnology

Quarta M,Brett JO,DiMarco R,De Morree A,Boutet SC,Chacon R,Gibbons MC,Garcia VA,Su J,Shrager JB,Heilshorn S,Rando TA

MHCD4520 was used in flow cytometry to develop an artificial niche to maintain muscle stem cells in a potent and quiescent state

Fri Jul 01 00:00:00 EDT 2016

Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.

Mucosal immunology

Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M

MHCD4520 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.

Fri Jan 01 00:00:00 EST 2016

Deformability-based cell selection with downstream immunofluorescence analysis.

Integrative biology : quantitative biosciences from nano to macro

Shaw Bagnall J,Byun S,Miyamoto DT,Kang JH,Maheswaran S,Stott SL,Toner M,Manalis SR

MHCD4520 was used in immunocytochemistry to develop a cell sorting technique for use with a suspended microchannel resonator that measures the biophysical characteristics of a single cell

Mon May 16 00:00:00 EDT 2016

Deformability-based cell selection with downstream immunofluorescence analysis.

Integrative biology : quantitative biosciences from nano to macro

Shaw Bagnall J,Byun S,Miyamoto DT,Kang JH,Maheswaran S,Stott SL,Toner M,Manalis SR

MHCD4520 was used in immunocytochemistry to develop a cell sorting technique for use with a suspended microchannel resonator that measures the biophysical characteristics of a single cell

Mon May 16 00:00:00 EDT 2016

Deformability of Tumor Cells versus Blood Cells.

Scientific reports

Shaw Bagnall J,Byun S,Begum S,Miyamoto DT,Hecht VC,Maheswaran S,Stott SL,Toner M,Hynes RO,Manalis SR

Fri Dec 18 00:00:00 EST 2015

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 488

CD45 Antibody (MHCD4520) in ICC/IF

Product Details

MHCD4520

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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