Invitrogen

CD45 Monoclonal Antibody (HI30), PerCP

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2 Published Figures

14 References

View all (406) CD45 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 4

CD45 Antibody (MHCD4531) in Flow

Human peripheral blood lymphocytes were stained with PerCP conjugated anti-human CD45 monoclonal antibody (Product # MHCD4531). The negative profile represents unstained cells.

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

Product Details

MHCD4531

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 12 publications 12 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HI30

Immunogen

Human CD45

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with sucrose, 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373115

Product Specific Information

PerCP conjugates are excited by the 488 nm line of the argon-ion laser and have a large Stokes shift, providing minimal spectral overlap into the R-PE (phycoerythrin) channel.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Analyte Specific Reagent

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Product References

Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children.

Nature immunology

Xu Q,Milanez-Almeida P,Martins AJ,Radtke AJ,Hoehn KB,Oguz C,Chen J,Liu C,Tang J,Grubbs G,Stein S,Ramelli S,Kabat J,Behzadpour H,Karkanitsa M,Spathies J,Kalish H,Kardava L,Kirby M,Cheung F,Preite S,Duncker PC,Kitakule MM,Romero N,Preciado D,Gitman L,Koroleva G,Smith G,Shaffer A,McBain IT,McGuire PJ,Pittaluga S,Germain RN,Apps R,Schwartz DM,Sadtler K,Moir S,Chertow DS,Kleinstein SH,Khurana S,Tsang JS,Mudd P,Schwartzberg PL,Manthiram K

MHCD4531 was used in Flow cytometry/Cell sorting to provide evidence for persistent tissue-specific immunity to SARS-CoV-2 in the upper respiratory tract of children after infection.

Sun Jan 01 00:00:00 EST 2023

The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject's Individual Age, Sex, Body Mass Index and Ethnicity.

Cells

Schmitz C,Alt C,Azares AR,Pearce DA,Facile TR,Furia JP,Maffulli N,Huang C,Alt EU

MHCD4531 was used in Flow cytometry/Cell sorting to show that key characteristics of unmodified, autologous, adipose-derived regenerative cells (UA-ADRCs) can be independent of the subject's age, sex, BMI and ethnicity.

Wed Dec 21 00:00:00 EST 2022

Mucosal-associated invariant T cell responses differ by sex in COVID-19.

Med (New York, N.Y.)

Yu C,Littleton S,Giroux NS,Mathew R,Ding S,Kalnitsky J,Yang Y,Petzold E,Chung HA,Rivera GO,Rotstein T,Xi R,Ko ER,Tsalik EL,Sempowski GD,Denny TN,Burke TW,McClain MT,Woods CW,Shen X,Saban DR

MHCD4531 was used in Flow Cytometry to reveal a pronounced reduction of circulating mucosal-associated invariant T (MAIT) cells in infected females.

Fri Jun 11 00:00:00 EDT 2021

The Presence of Survivin on B Cells from Myasthenia Gravis Patients and the Potential of an Antibody to a Modified Survivin Peptide to Alleviate Weakness in an Animal Model.

Journal of immunology (Baltimore, Md. : 1950)

Zhang X,Ciesielski M,Fenstermaker RA,Kaminski HJ,Kusner LL

MHCD4531 was used in Flow cytometry/Cell sorting to define the role of survivin in myasthenia gravis by assessing PBMCs from 29 patients with MG and 15 controls.

Thu Oct 01 00:00:00 EDT 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

MHCD4531 was used in Flow cytometry/Cell sorting to investigate the effects of normoxic and hypoxic culture condition on early- and late-passage adipose-derived MSCs.

Tue Oct 06 00:00:00 EDT 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

MHCD4531 was used in Flow cytometry/Cell sorting to investigate the effects of normoxic and hypoxic culture condition on early- and late-passage adipose-derived MSCs.

Tue Oct 06 00:00:00 EDT 2020

Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection.

Journal of neuroinflammation

Mahmud FJ,Du Y,Greif E,Boucher T,Dannals RF,Mathews WB,Pomper MG,Sysa-Shah P,Metcalf Pate KA,Lyons C,Carlson B,Chacona M,Brown AM

MHCD4531 was used in Flow cytometry/Cell sorting to propose that osteopontin may act as a molecular brake within the brain in order to regulate the inflammatory response to chronic viral infections, such as HIV.

Thu Sep 17 00:00:00 EDT 2020

Leukocyte-Derived Extracellular Vesicles in Blood with and without EpCAM Enrichment.

Cells

Nanou A,Zeune LL,Terstappen LWMM

MHCD4531 was used in Immunohistochemistry to establish the negative association of the presence of large tumor-derived Extracellular Vesicles to overall survival of cancer patients.

Tue Aug 20 00:00:00 EDT 2019

Toward a real liquid biopsy in metastatic breast and prostate cancer: Diagnostic LeukApheresis increases CTC yields in a European prospective multicenter study (CTCTrap).

International journal of cancer

Andree KC,Mentink A,Zeune LL,Terstappen LWMM,Stoecklein NH,Neves RP,Driemel C,Lampignano R,Yang L,Neubauer H,Fehm T,Fischer JC,Rossi E,Manicone M,Basso U,Marson P,Zamarchi R,Loriot Y,Lapierre V,Faugeroux V,Oulhen M,Farace F,Fowler G,Sousa Fontes M,Ebbs B,Lambros M,Crespo M,Flohr P,de Bono JS

MHCD4531 was used to aim to validate and optimize the recently introduced Diagnostic LeukApheresis to screen liters of blood.

Thu Nov 15 00:00:00 EST 2018

Scanning Electron Microscopy of Circulating Tumor Cells and Tumor-Derived Extracellular Vesicles.

Cancers

Nanou A,Crespo M,Flohr P,De Bono JS,Terstappen LWMM

MHCD4531 was used in Flow cytometry/Cell sorting to explore the morphological features of circulating tumour cells and tumour-derived extracellular vesicles.

Wed Oct 31 00:00:00 EDT 2018

Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells.

PLoS neglected tropical diseases

Salerno-Gonçalves R,Tettelin H,Lou D,Steiner S,Rezwanul T,Guo Q,Picking WD,Nene V,Sztein MB

MHCD4531 was used in Flow cytometry/Cell sorting to demonstrate the feasibility of using an E. coli expression system to uncover the antigen specificity of T-cells, highlighting its applicability to vaccine studies.

Fri Sep 01 00:00:00 EDT 2017

CD40 Expression in Fibrocytes Is Induced by TSH: Potential Synergistic Immune Activation.

PloS one

Mester T,Raychaudhuri N,Gillespie EF,Chen H,Smith TJ,Douglas RS

MHCD4531 was used in flow cytometry discuss the potential roles of TSHR and CD40 in thyroid-associated ophthalmopathy

Wed Aug 02 00:00:00 EDT 2017

Teprotumumab, an IGF-1R blocking monoclonal antibody inhibits TSH and IGF-1 action in fibrocytes.

The Journal of clinical endocrinology and metabolism

Chen H,Mester T,Raychaudhuri N,Kauh CY,Gupta S,Smith TJ,Douglas RS

MHCD4531 was used in flow cytometry to describe the phase II trial using teprotumumab (RV 001, R1507) to treat patients with active thyroid-associated ophthalmopathy

Mon Sep 01 00:00:00 EDT 2014

Accurate quantitation of D+ fetomaternal hemorrhage by flow cytometry using a novel reagent to eliminate granulocytes from analysis.

Transfusion

Kumpel B,Hazell M,Guest A,Dixey J,Mushens R,Bishop D,Wreford-Bush T,Lee E

MHCD4531 was used in flow cytometry to discuss methods to detect fetomaternal hemorrhage.

Thu May 01 00:00:00 EDT 2014

Thyrotropin receptor and CD40 mediate interleukin-8 expression in fibrocytes: implications for thyroid-associated ophthalmopathy (an American Ophthalmological Society thesis).

Transactions of the American Ophthalmological Society

Douglas RS,Mester T,Ginter A,Kim DS

MHCD4531 was used in flow cytometry to characterize the thyrotropin receptor, CD40, and IL-8 in peripheral blood fibrocytes of patients with thyroid-associated orbitopathy

Thu Feb 12 00:00:00 EST 2015

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45 Monoclonal Antibody (HI30), PerCP

CD45 Antibody (MHCD4531) in Flow

Product Details

MHCD4531

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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