Invitrogen

CD45 Monoclonal Antibody (HI30), APC-Alexa Fluor™ 750

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

2 Published Figures

7 References

View all (406) CD45 antibodies

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FIGURE: 1 / 3

CD45 Antibody (MHCD4527) in Flow

Cytometry. Part A : the journal of the International Society for Analytical Cytology 2010 - Published figure using CD45 monoclonal antibody (Product # MHCD4527) in Flow Cytometry

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

Product Details

MHCD4527

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HI30

Immunogen

CD45 antigen

Conjugate

APC-Alexa Fluor™ 750 APC-Alexa Fluor™ 750 APC-Alexa Fluor™ 750

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with sucrose, BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372213

Product Specific Information

The allophycocyanin (APC)-Alexa Fluor® 750 dye tandem conjugate allows for multicolor measurements using excitation by lasers that emit from 633 to 650 nm.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Analyte Specific Reagent

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Product References

Novel Markers of Angiogenesis in the Setting of Cognitive Impairment and Dementia.

Journal of Alzheimer's disease : JAD

Callahan CM,Apostolova LG,Gao S,Risacher SL,Case J,Saykin AJ,Lane KA,Swinford CG,Yoder MC

MHCD4527 was used in Flow cytometry/Cell sorting to explore the relationship between angiogenesis activity and evidence of neurodegeneration among older adults.

Mon May 10 00:00:00 EDT 2021

The interplay of BMP4 and IL‑7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia̸reperfusion.

International journal of molecular medicine

Luo B,Chen K,Feng Q,Xiao W,Ma D,Yang H,Zhang C

MHCD4527 was used in Flow cytometry/Cell sorting to investigate whether the interplay of bone morphogenetic protein 4 and IL-7 regulates intestinal intraepithelial lymphocyte function and number.

Tue May 01 00:00:00 EDT 2018

Weight loss achieved using an energy restriction diet with normal or higher dietary protein decreased the number of CD14⁺⁺CD16⁺ proinflammatory monocytes and plasma lipids and lipoproteins in middle-aged, overweight, and obese adults.

Nutrition research (New York, N.Y.)

Kim JE,Lin G,Zhou J,Mund JA,Case J,Campbell WW

MHCD4527 was used in flow cytometry to test if high protein affects monocytes during weight loss

Sat Apr 01 00:00:00 EDT 2017

Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.

Mucosal immunology

Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M

MHCD4527 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.

Fri Jan 01 00:00:00 EST 2016

Endothelial abnormalities in adolescents with type 1 diabetes: a biomarker for vascular sequelae?

The Journal of pediatrics

DiMeglio LA,Tosh A,Saha C,Estes M,Mund J,Mead LE,Lien I,Ingram DA,Haneline LS

Published figure using CD45 monoclonal antibody (Product # MHCD4527) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2010

Application of polychromatic flow cytometry to identify novel subsets of circulating cells with angiogenic potential.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Estes ML,Mund JA,Mead LE,Prater DN,Cai S,Wang H,Pollok KE,Murphy MP,An CS,Srour EF,Ingram DA,Case J

MHCD4527 was used in flow cytometry to report that functional circulating hematopoietic stem and progenitor cells can be separated from other cell populations based on AC133 expression

Wed Sep 01 00:00:00 EDT 2010

Identification of endothelial cells and progenitor cell subsets in human peripheral blood.

Current protocols in cytometry

Estes ML,Mund JA,Ingram DA,Case J

MHCD4527 was used in Flow Cytometry to investigate the roles of human hematopoietic and endothelial elements in the growth and maintenance of the vasculature.

Thu Apr 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45 Monoclonal Antibody (HI30), APC-Alexa Fluor™ 750

CD45 Antibody (MHCD4527) in Flow

Product Details

MHCD4527

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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