Invitrogen

CD45R (B220) Monoclonal Antibody (RA3-6B2), PE-Cyanine5, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

76 Published Figures

114 References

View all (99) B220 (CD45R) antibodies

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Datasheet

Protocols

Questions & Answers

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CD45R (B220) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 78

CD45R (B220) Antibody (15-0452-82) in Flow

Staining of BALB/c splenocytes with 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine5 (Product # 15-4321-80) (blue histogram) or 0.06 µg of Anti-Human/Mouse CD45R (B220) PE-Cyanine5 (purple histogram).Cells in the lymphocyte gate were used for analysis.

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Product Details

15-0452-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 110 publications 110 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RA3-6B2

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468755

Product Specific Information

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the I{lpr/lprI} mutant mouse also express this antigen.

Applications Reported: The RA3-6B2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Platelet factors attenuate inflammation and rescue cognition in ageing.

Nature

Schroer AB,Ventura PB,Sucharov J,Misra R,Chui MKK,Bieri G,Horowitz AM,Smith LK,Encabo K,Tenggara I,Couthouis J,Gross JD,Chan JM,Luke A,Villeda SA

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Platelet factors attenuate inflammation and rescue cognition in ageing.

Nature

Schroer AB,Ventura PB,Sucharov J,Misra R,Chui MKK,Bieri G,Horowitz AM,Smith LK,Encabo K,Tenggara I,Couthouis J,Gross JD,Chan JM,Luke A,Villeda SA

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Stromal niche inflammation mediated by IL-1 signalling is a targetable driver of haematopoietic ageing.

Nature cell biology

Mitchell CA,Verovskaya EV,Calero-Nieto FJ,Olson OC,Swann JW,Wang X,Hérault A,Dellorusso PV,Zhang SY,Svendsen AF,Pietras EM,Bakker ST,Ho TT,Göttgens B,Passegué E

15-0452-82 was used in Flow cytometry/Cell sorting to characterize the inflammatory milieu that drives both niche and haematopoietic remodelling.

Sun Jan 01 00:00:00 EST 2023

Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.

JCI insight

Zhong L,Yao L,Holdreith N,Yu W,Gui T,Miao Z,Elkaim Y,Li M,Gong Y,Pacifici M,Maity A,Busch TM,Joeng KS,Cengel K,Seale P,Tong W,Qin L

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma.

Cell death & disease

Sun Q,Wang Y,Ji H,Sun X,Xie S,Chen L,Li S,Zeng W,Chen R,Tang Q,Zuo J,Hou L,Hosaka K,Lu Y,Liu Y,Ye Y,Yang Y

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Aug 19 00:00:00 EDT 2022

Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis.

Science advances

Ma L,Tang Q,Gao X,Lee J,Lei R,Suzuki M,Zheng D,Ito K,Frenette PS,Dawlaty MM

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress.

Scientific reports

Martín-Hernández D,Gutiérrez IL,González-Prieto M,MacDowell KS,Robledo-Montaña J,Tendilla-Beltrán H,Calleja-Rodríguez N,Bris ÁG,Ulecia-Morón C,Moreno B,Caso JR,García-Bueno B,Rodrigues-Mascarenhas S,Marín-Jiménez I,Leza JC,Menchén L

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2022

Neutrophil "plucking" on megakaryocytes drives platelet production and boosts cardiovascular disease.

Immunity

Petzold T,Zhang Z,Ballesteros I,Saleh I,Polzin A,Thienel M,Liu L,Ul Ain Q,Ehreiser V,Weber C,Kilani B,Mertsch P,Götschke J,Cremer S,Fu W,Lorenz M,Ishikawa-Ankerhold H,Raatz E,El-Nemr S,Görlach A,Marhuenda E,Stark K,Pircher J,Stegner D,Gieger C,Schmidt-Supprian M,Gaertner F,Almendros I,Kelm M,Schulz C,Hidalgo A,Massberg S

15-0452-82 was used in Flow cytometry/Cell sorting to establish neutrophil plucking as a target to reduce thromboischemic events.

Tue Dec 13 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

γ-Tocotrienol-Loaded Liposomes for Radioprotection from Hematopoietic Side Effects Caused by Radiotherapeutic Drugs.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine

Lee SG,Kalidindi TM,Lou H,Gangangari K,Punzalan B,Bitton A,Lee CJ,Vargas HA,Park S,Bodei L,Kharas MG,Singh VK,Kishore Pillarsetty NV,Larson SM

15-0452-83 was used in Flow Cytometry to report a novel radioprotector drug, a liposomal formulation of gamma-tocotrienol (GT3), or GT3-Nano for short, to mitigate bone marrow radiation damage during targeted radionuclide therapy (TRT).

Thu Apr 01 00:00:00 EDT 2021

A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system.

Computational and structural biotechnology journal

Shen Y,Jiang L,Iyer VS,Raposo B,Dubnovitsky A,Boddul SV,Kasza Z,Wermeling F

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Wed Nov 10 00:00:00 EST 2021

Defining the Mechanistic Correlates of Protection Conferred by Whole-Cell Vaccination against Pseudomonas aeruginosa Acute Murine Pneumonia.

Infection and immunity

Sen-Kilic E,Blackwood CB,Huckaby AB,Horspool AM,Weaver KL,Malkowski AC,Witt WT,Bevere JR,Damron FH,Barbier M

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2021

Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.

Stem cell research

Sudo K,Yamazaki S,Wilkinson AC,Nakauchi H,Nakamura Y

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Metabolic preconditioning in CD4+ T cells restores inducible immune tolerance in lupus-prone mice.

JCI insight

Wilson CS,Stocks BT,Hoopes EM,Rhoads JP,McNew KL,Major AS,Moore DJ

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Oct 08 00:00:00 EDT 2021

The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation.

Nature communications

Shang M,Yang H,Yang R,Chen T,Fu Y,Li Y,Fang X,Zhang K,Zhang J,Li H,Cao X,Gu J,Xiao J,Zhang Q,Liu X,Yu Q,Wang T

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Mon Mar 29 00:00:00 EDT 2021

Type 2 diabetic mice enter a state of spontaneous hibernation-like suspended animation following accumulation of uric acid.

The Journal of biological chemistry

Zhao Y,Cheng R,Zhao Y,Ge W,Yang Y,Ding Z,Xu X,Wang Z,Wu Z,Zhang J

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Lederer K,Castaño D,Gómez Atria D,Oguin TH,Wang S,Manzoni TB,Muramatsu H,Hogan MJ,Amanat F,Cherubin P,Lundgreen KA,Tam YK,Fan SHY,Eisenlohr LC,Maillard I,Weissman D,Bates P,Krammer F,Sempowski GD,Pardi N,Locci M

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Dec 15 00:00:00 EST 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

DNA-PKcs phosphorylation at the T2609 cluster alters the repair pathway choice during immunoglobulin class switch recombination.

Proceedings of the National Academy of Sciences of the United States of America

Crowe JL,Wang XS,Shao Z,Lee BJ,Estes VM,Zha S

15-0452 was used in Flow cytometry/Cell sorting to uncover a role of DNA-PKcs T2609 phosphorylation in promoting cNHEJ repair pathway choice during CSR.

Tue Sep 15 00:00:00 EDT 2020

Conditional antibody expression to avoid central B cell deletion in humanized HIV-1 vaccine mouse models.

Proceedings of the National Academy of Sciences of the United States of America

Tian M,McGovern K,Cheng HL,Waddicor P,Rieble L,Dao M,Chen Y,Kimble MT,Cantor E,Manfredonia N,Judson R,Chapdelaine-Williams A,Cain DW,Haynes BF,Alt FW

15-0452 was used in Flow cytometry/Cell sorting to develop a new approach to test a vaccine which circumvents B cell developmental blocks.

Tue Apr 07 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection.

Frontiers in immunology

Larson SR,Bortell N,Illies A,Crisler WJ,Matsuda JL,Lenz LL

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity.

Frontiers in immunology

Myers DR,Abram CL,Wildes D,Belwafa A,Welsh AMN,Schulze CJ,Choy TJ,Nguyen T,Omaque N,Hu Y,Singh M,Hansen R,Goldsmith MA,Quintana E,Smith JAM,Lowell CA

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Mon Jun 14 00:00:00 EDT 2021

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells.

Frontiers in pharmacology

Guo C,Ran Q,Sun C,Zhou T,Yang X,Zhang J,Pang S,Xiao Y

15-0452-82 was used in Flow Cytometry to find that FGFR3 had a novel regulatory mechanism for the stemness of LSCs in AML, and provided a promising anti-leukemia approach by interrupting FGFR3.

Tue Feb 16 00:00:00 EST 2021

HyperTRIBE uncovers increased MUSASHI-2 RNA binding activity and differential regulation in leukemic stem cells.

Nature communications

Nguyen DTT,Lu Y,Chu KL,Yang X,Park SM,Choo ZN,Chin CR,Prieto C,Schurer A,Barin E,Savino AM,Gourkanti S,Patel P,Vu LP,Leslie CS,Kharas MG

15-0452 was used in Flow cytometry/Cell sorting to globally map the mRNA targets of the RBP MSI2 in mammalian adult normal and malignant stem cells.

Fri Apr 24 00:00:00 EDT 2020

Phosphorylation at S2053 in Murine (S2056 in Human) DNA-PKcs Is Dispensable for Lymphocyte Development and Class Switch Recombination.

Journal of immunology (Baltimore, Md. : 1950)

Jiang W,Estes VM,Wang XS,Shao Z,Lee BJ,Lin X,Crowe JL,Zha S

15-0452-82 was used in Flow Cytometry to show that radiation can induce phosphorylation of murine DNA-PKcs at the corresponding S2053.

Mon Jul 01 00:00:00 EDT 2019

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.

Frontiers in immunology

Xu J,Pei S,Wang Y,Liu J,Qian Y,Huang M,Zhang Y,Xiao Y

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Wed Oct 14 00:00:00 EDT 2020

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue May 14 00:00:00 EDT 2019

The Hematopoietic Oxidase NOX2 Regulates Self-Renewal of Leukemic Stem Cells.

Cell reports

Adane B,Ye H,Khan N,Pei S,Minhajuddin M,Stevens BM,Jones CL,D'Alessandro A,Reisz JA,Zaberezhnyy V,Gasparetto M,Ho TC,Kelly KK,Myers JR,Ashton JM,Siegenthaler J,Kume T,Campbell EL,Pollyea DA,Becker MW,Jordan CT

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

m⁶A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment.

Cell reports

Cheng Y,Luo H,Izzo F,Pickering BF,Nguyen D,Myers R,Schurer A,Gourkanti S,Brüning JC,Vu LP,Jaffrey SR,Landau DA,Kharas MG

15-0452-82 was used in Flow cytometry/Cell sorting to indicate that RNA methylation controls symmetric commitment and cell identity of hematopoietic stem cells and may provide a general mechanism for how stem cells regulate differentiation fate choice.

Tue Aug 13 00:00:00 EDT 2019

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Cell reports

Balzano M,De Grandis M,Vu Manh TP,Chasson L,Bardin F,Farina A,Sergé A,Bidaut G,Charbord P,Hérault L,Bailly AL,Cartier-Michaud A,Boned A,Dalod M,Duprez E,Genever P,Coles M,Bajenoff M,Xerri L,Aurrand-Lions M,Schiff C,Mancini SJC

15-0452 was used in Flow cytometry/Cell sorting to study pro-B cells in their support of stroll cells and their interaction network allowing their retention.

Tue Mar 19 00:00:00 EDT 2019

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

Proapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.

Frontiers in immunology

Liu R,King A,Bouillet P,Tarlinton DM,Strasser A,Heierhorst J

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Host Tumor Suppressor p18^INK4c Functions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis.

Journal of virology

Niemeyer BF,Oko LM,Medina EM,Oldenburg DG,White DW,Cool CD,Clambey ET,van Dyk LF

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.

Experimental and therapeutic medicine

Li Z,Zhang S,Cao L,Li W,Ye YC,Shi ZX,Wang ZR,Sun LX,Wang JW,Jia LT,Wang W

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice.

Frontiers in immunology

Perez-Chacon G,Adrados M,Vallejo-Cremades MT,Lefebvre S,Reed JC,Zapata JM

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Oct 25 00:00:00 EDT 2019

ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.

Frontiers in immunology

Glal D,Sudhakar JN,Lu HH,Liu MC,Chiang HY,Liu YC,Cheng CF,Shui JW

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

Retinoid-Sensitive Epigenetic Regulation of the Hoxb Cluster Maintains Normal Hematopoiesis and Inhibits Leukemogenesis.

Cell stem cell

Qian P,De Kumar B,He XC,Nolte C,Gogol M,Ahn Y,Chen S,Li Z,Xu H,Perry JM,Hu D,Tao F,Zhao M,Han Y,Hall K,Peak A,Paulson A,Zhao C,Venkatraman A,Box A,Perera A,Haug JS,Parmely T,Li H,Krumlauf R,Li L

15-0452 was used in Flow cytometry/Cell sorting to demonstrate roles for retinoid signaling and the DERARE in maintaining HSCs and preventing leukemogenesis by coordinate regulation of Hoxb genes.

Thu May 03 00:00:00 EDT 2018

Dual Strategies for Argonaute2-Mediated Biogenesis of Erythroid miRNAs Underlie Conserved Requirements for Slicing in Mammals.

Molecular cell

Jee D,Yang JS,Park SM,Farmer DT,Wen J,Chou T,Chow A,McManus MT,Kharas MG,Lai EC

15-0452-82 was used in flow cytometry to investigate erythroid miRNA biogenesis in mammalian slicing.

Thu Jan 18 00:00:00 EST 2018

Direct Activation of BAX by BTSA1 Overcomes Apoptosis Resistance in Acute Myeloid Leukemia.

Cancer cell

Reyna DE,Garner TP,Lopez A,Kopp F,Choudhary GS,Sridharan A,Narayanagari SR,Mitchell K,Dong B,Bartholdy BA,Walensky LD,Verma A,Steidl U,Gavathiotis E

15-0452 was used in Flow cytometry/Cell sorting to identify BTSA1, a pharmacologically optimised BAX activator that binds with high affinity and specificity to the N-terminal activation site and induces conformational changes to BAX leading to BAX-mediated apoptosis.

Mon Oct 09 00:00:00 EDT 2017

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.

The Journal of experimental medicine

Takahashi T,Asano Y,Sugawara K,Yamashita T,Nakamura K,Saigusa R,Ichimura Y,Toyama T,Taniguchi T,Akamata K,Noda S,Yoshizaki A,Tsuruta D,Trojanowska M,Sato S

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Mon Apr 03 00:00:00 EDT 2017

EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.

Nature communications

Béguelin W,Rivas MA,Calvo Fernández MT,Teater M,Purwada A,Redmond D,Shen H,Challman MF,Elemento O,Singh A,Melnick AM

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2017

DNA-binding of the Tet-transactivator curtails antigen-induced lymphocyte activation in mice.

Nature communications

Ottina E,Peperzak V,Schoeler K,Carrington E,Sgonc R,Pellegrini M,Preston S,Herold MJ,Strasser A,Villunger A

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2017

The Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis.

Oncoimmunology

Dagenais M,Dupaul-Chicoine J,Douglas T,Champagne C,Morizot A,Saleh M

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.

JCI insight

Azzam KM,Madenspacher JH,Cain DW,Lai L,Gowdy KM,Rai P,Janardhan K,Clayton N,Cunningham W,Jensen H,Patel PS,Kearney JF,Taylor GA,Fessler MB

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.

PloS one

Komegae EN,Souza TA,Grund LZ,Lima C,Lopes-Ferreira M

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

IgE-mediated enhancement of CD4(+) T cell responses requires antigen presentation by CD8α(-) conventional dendritic cells.

Scientific reports

Ding Z,Dahlin JS,Xu H,Heyman B

15-0452 was used in Flow cytometry/Cell sorting to elucidate the dendritic cell population involved in the presentation of IgE-Ag complexes during enhanced specific antibody and CD4+ T cell responses.

Thu Jun 16 00:00:00 EDT 2016

Chk1 inhibition significantly potentiates activity of nucleoside analogs in TP53-mutated B-lymphoid cells.

Oncotarget

Zemanova J,Hylse O,Collakova J,Vesely P,Oltova A,Borsky M,Zaprazna K,Kasparkova M,Janovska P,Verner J,Kohoutek J,Dzimkova M,Bryja V,Jaskova Z,Brychtova Y,Paruch K,Trbusek M

15-0452 was used in Flow cytometry/Cell sorting to compare the effects of adding a Chk1 inhibitor to nucleoside analogs in p53-proficient and p53-defective cells.

Tue Sep 20 00:00:00 EDT 2016

Clec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton.

eLife

Yue R,Shen B,Morrison SJ

15-0452 was used in Flow cytometry/Cell sorting to study how Clec11a/Osteolectin maintains the adult skeleton by promoting the differentiation of mesenchymal progenitors into mature osteoblasts.

Tue Dec 13 00:00:00 EST 2016

Alkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity.

PloS one

Jonas BA,Johnson C,Gratzinger D,Majeti R

15-0452 was used in Flow cytometry/Cell sorting to report several new t-AML/MDS mouse models that could potentially be used to further define disease pathogenesis and test novel therapeutics.

Wed Jul 26 00:00:00 EDT 2017

Aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors.

The Journal of clinical investigation

Henry CJ,Casás-Selves M,Kim J,Zaberezhnyy V,Aghili L,Daniel AE,Jimenez L,Azam T,McNamee EN,Clambey ET,Klawitter J,Serkova NJ,Tan AC,Dinarello CA,DeGregori J

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

An anti-silencer- and SATB1-dependent chromatin hub regulates Rag1 and Rag2 gene expression during thymocyte development.

The Journal of experimental medicine

Hao B,Naik AK,Watanabe A,Tanaka H,Chen L,Richards HW,Kondo M,Taniuchi I,Kohwi Y,Kohwi-Shigematsu T,Krangel MS

15-0452 was used in Flow cytometry/Cell sorting to provide a novel framework for understanding ASE function for gene expression in double-positive thymocytes.

Mon May 04 00:00:00 EDT 2015

Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.

Cell death and differentiation

Cho J,Seo J,Lim CH,Yang L,Shiratsuchi T,Lee MH,Chowdhury RR,Kasahara H,Kim JS,Oh SP,Lee YJ,Terada N

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Overexpression of Bovine FcRn in Mice Enhances T-Dependent Immune Responses by Amplifying T Helper Cell Frequency and Germinal Center Enlargement in the Spleen.

Frontiers in immunology

Schneider Z,Jani PK,Szikora B,Végh A,Kövesdi D,Iliás A,Cervenak J,Balogh P,Kurucz I,Kacskovics I

15-0452 was used in Flow cytometry/Cell sorting to demonstrate that Tg mice that overexpress bFcRn developed efficient immune response against very low dose of antigen.

Mon Aug 10 00:00:00 EDT 2015

Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection.

PLoS pathogens

Wiesner DL,Specht CA,Lee CK,Smith KD,Mukaremera L,Lee ST,Lee CG,Elias JA,Nielsen JN,Boulware DR,Bohjanen PR,Jenkins MK,Levitz SM,Nielsen K

15-0452 was used in Flow cytometry/Cell sorting to investigate Th2 cell responses to pulmonary fungal infection, showing that chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection.

Sun Mar 01 00:00:00 EST 2015

Isolation and immunomodulatory activity of bursal peptide, a novel bursal peptide from the chicken bursa of Fabricius.

Journal of veterinary science

Liu XD,Qian Y,Jung YS,Chen PY

15-0452 was used in Flow cytometry/Cell sorting to provide novel insights into the use of a potential candidate reagent for B cell development and future immuno-pharmacological use.

Thu Sep 22 00:00:00 EDT 2016

Endothelial Gata5 transcription factor regulates blood pressure.

Nature communications

Messaoudi S,He Y,Gutsol A,Wight A,Hébert RL,Vilmundarson RO,Makrigiannis AP,Chalmers J,Hamet P,Tremblay J,McPherson R,Stewart AFR,Touyz RM,Nemer M

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Mon Nov 30 00:00:00 EST 2015

Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.

Nature communications

Singh NK,Kotla S,Dyukova E,Traylor JG,Orr AW,Chernoff J,Marion TN,Rao GN

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Wed Jun 24 00:00:00 EDT 2015

Sleep disruption impairs haematopoietic stem cell transplantation in mice.

Nature communications

Rolls A,Pang WW,Ibarra I,Colas D,Bonnavion P,Korin B,Heller HC,Weissman IL,de Lecea L

15-0452 was used in Flow cytometry/Cell sorting to provide insights into cellular and molecular mechanisms underlying the effects of sleep deprivation on HSCs.

Wed Oct 14 00:00:00 EDT 2015

Infection mobilizes hematopoietic stem cells through cooperative NOD-like receptor and Toll-like receptor signaling.

Cell host & microbe

Burberry A,Zeng MY,Ding L,Wicks I,Inohara N,Morrison SJ,Núñez G

15-0452 was used in Flow cytometry/Cell sorting to investigate how infection mobilises hematopoietic stem cells through cooperative NOD-like receptor and Toll-like receptor signalling.

Wed Jun 11 00:00:00 EDT 2014

The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids.

Nature immunology

Agudo J,Ruzo A,Tung N,Salmon H,Leboeuf M,Hashimoto D,Becker C,Garrett-Sinha LA,Baccarini A,Merad M,Brown BD

15-0452 was used in Western Blotting to identify the miR-126-VEGFR2 axis as an important regulator of the innate response that operates through multiscale control of pDCs.

Wed Jan 01 00:00:00 EST 2014

The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids.

Nature immunology

Agudo J,Ruzo A,Tung N,Salmon H,Leboeuf M,Hashimoto D,Becker C,Garrett-Sinha LA,Baccarini A,Merad M,Brown BD

15-0452 was used in Western Blotting to identify the miR-126-VEGFR2 axis as an important regulator of the innate response that operates through multiscale control of pDCs.

Wed Jan 01 00:00:00 EST 2014

Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response.

Journal of immunology (Baltimore, Md. : 1950)

Park KS,Bayles I,Szlachta-McGinn A,Paul J,Boiko J,Santos P,Liu J,Wang Z,Borghesi L,Milcarek C

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Sat Nov 01 00:00:00 EDT 2014

IκBε is a key regulator of B cell expansion by providing negative feedback on cRel and RelA in a stimulus-specific manner.

Journal of immunology (Baltimore, Md. : 1950)

Alves BN,Tsui R,Almaden J,Shokhirev MN,Davis-Turak J,Fujimoto J,Birnbaum H,Ponomarenko J,Hoffmann A

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Mouse mast cell protease-6 and MHC are involved in the development of experimental asthma.

Journal of immunology (Baltimore, Md. : 1950)

Cui Y,Dahlin JS,Feinstein R,Bankova LG,Xing W,Shin K,Gurish MF,Hallgren J

15-0452 was used in Flow cytometry/Cell sorting to compare the immune responses of BALB/c mice to mMCP-6(-/-) and mMCP-6(-/-)/H-2bc mice in a mouse model of experimental asthma.

Sat Nov 15 00:00:00 EST 2014

Rhof promotes murine marginal zone B cell development.

Nagoya journal of medical science

Kishimoto M,Matsuda T,Yanase S,Katsumi A,Suzuki N,Ikejiri M,Takagi A,Ikawa M,Kojima T,Kunishima S,Kiyoi H,Naoe T,Matsushita T,Maruyama M

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling.

PloS one

Kasama Y,Mizukami T,Kusunoki H,Peveling-Oberhag J,Nishito Y,Ozawa M,Kohara M,Mizuochi T,Tsukiyama-Kohara K

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Mon Dec 22 00:00:00 EST 2014

Usp16 contributes to somatic stem-cell defects in Down's syndrome.

Nature

Adorno M,Sikandar S,Mitra SS,Kuo A,Nicolis Di Robilant B,Haro-Acosta V,Ouadah Y,Quarta M,Rodriguez J,Qian D,Reddy VM,Cheshier S,Garner CC,Clarke MF

15-0452 was used in Flow cytometry/Cell sorting to demonstrate a role for USP16 in antagonising the self-renewal and senescence pathways in Down's syndrome.

Thu Sep 19 00:00:00 EDT 2013

FOXO3A directs a protective autophagy program in haematopoietic stem cells.

Nature

Warr MR,Binnewies M,Flach J,Reynaud D,Garg T,Malhotra R,Debnath J,Passegué E

15-0452 was used in Flow cytometry/Cell sorting to show how FOXO3A directs a protective autophagy program in haematopoietic stem cells.

Thu Feb 21 00:00:00 EST 2013

Reciprocal effects of rab7 deletion in activated and neglected T cells.

Autophagy

Roy SG,Stevens MW,So L,Edinger AL

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2013

Both NK cell-intrinsic and -extrinsic STAT1 signaling are required for NK cell response against vaccinia virus.

Journal of immunology (Baltimore, Md. : 1950)

Fortin C,Huang X,Yang Y

15-0452 was used in Flow cytometry/Cell sorting to explore the role of STAT1 in natural killer cell responses to vaccinia virus infection in vivo.

Mon Jul 01 00:00:00 EDT 2013

Coactivator-associated arginine methyltransferase 1 regulates fetal hematopoiesis and thymocyte development.

Journal of immunology (Baltimore, Md. : 1950)

Li J,Zhao Z,Carter C,Ehrlich LI,Bedford MT,Richie ER

15-0452 was used in Flow cytometry/Cell sorting to demonstrate how CARM1 is a key epigenetic regulator of hematopoiesis that affects multiple lineages at various stages of differentiation.

Tue Jan 15 00:00:00 EST 2013

Flow cytometry analysis of murine hematopoietic stem cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Mayle A,Luo M,Jeong M,Goodell MA

15-0452 was used in Flow cytometry/Cell sorting to discuss methods for rapid flow cytometric analysis of peripheral blood cell types and novel strategies for working with rare cell populations such as hematopoietic stem cells in the analysis of cell cycle status by BrdU, Ki-67, and Pyronin Y staining.

Tue Jan 01 00:00:00 EST 2013

TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

PLoS genetics

Gómez-Herreros F,Romero-Granados R,Zeng Z,Alvarez-Quilón A,Quintero C,Ju L,Umans L,Vermeire L,Huylebroeck D,Caldecott KW,Cortés-Ledesma F

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

Partial depletion of regulatory T cells does not influence the inflammation caused by high dose hemi-body irradiation.

PloS one

Ma S,Richardson JA,Bitmansour A,Solberg TD,Pidikiti R,Song K,Stojadinovic S,Vitetta ES,Meyer JJ

15-0452 was used in Flow cytometry/Cell sorting to investigate the effects of partial depletion of regulatory T cells alongside ionizing radiation on inflammation and tissue injury in mice.

Mon Jul 29 00:00:00 EDT 2013

Overexpression of Snai3 suppresses lymphoid- and enhances myeloid-cell differentiation.

European journal of immunology

Dahlem T,Cho S,Spangrude GJ,Weis JJ,Weis JH

15-0452 was used in Flow cytometry/Cell sorting to investigate the role of the transcriptional repressor Snai3 protein in the derivation of cells of the haematopoietic system.

Sun Apr 01 00:00:00 EDT 2012

Ymer acts as a multifunctional regulator in nuclear factor-κB and Fas signaling pathways.

Molecular medicine (Cambridge, Mass.)

Tsukiyama T,Matsuda-Tsukiyama M,Bohgaki M,Terai S,Tanaka S,Hatakeyama S

15-0452 was used in Flow cytometry/Cell sorting to explore the role of Ymer as a positive or negative regulator in several signalling pathways in mice.

Wed May 09 00:00:00 EDT 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

15-0452 was used in Flow cytometry/Cell sorting to demonstrate the essential role of Bcl10 in ensuring efficacy of BAFF, responsible for regulating NF-κB activation and subsequent survival of anergic B cells.

Sat Dec 01 00:00:00 EST 2012

Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase.

Journal of immunology (Baltimore, Md. : 1950)

Hasham MG,Snow KJ,Donghia NM,Branca JA,Lessard MD,Stavnezer J,Shopland LS,Mills KD

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2012

CD5-dependent CK2 activation pathway regulates threshold for T cell anergy.

Journal of immunology (Baltimore, Md. : 1950)

Sestero CM,McGuire DJ,De Sarno P,Brantley EC,Soldevila G,Axtell RC,Raman C

15-0452 was used in Flow cytometry/Cell sorting to determine the physiological significance of CD5-dependent casein kinase 2 activation in T cells.

Sat Sep 15 00:00:00 EDT 2012

CD11c+ cells are required for antigen-induced increase of mast cells in the lung.

Journal of immunology (Baltimore, Md. : 1950)

Dahlin JS,Feinstein R,Cui Y,Heyman B,Hallgren J

15-0452 was used in Flow cytometry/Cell sorting to determine the contribution of Ag-stimulated CD11c(+) cells to the allergic response in asthmatics.

Mon Oct 15 00:00:00 EDT 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Langerhans cells regulate cutaneous injury by licensing CD8 effector cells recruited to the skin.

Blood

Bennett CL,Fallah-Arani F,Conlan T,Trouillet C,Goold H,Chorro L,Flutter B,Means TK,Geissmann F,Chakraverty R

15-0452 was used in Flow cytometry/Cell sorting to explore the role of Langerhans cells in the development of tissue injury using a tractable model of cutaneous graft-versus-host disease.

Thu Jun 30 00:00:00 EDT 2011

Requirement for complement in antibody responses is not explained by the classic pathway activator IgM.

Proceedings of the National Academy of Sciences of the United States of America

Rutemark C,Alicot E,Bergman A,Ma M,Getahun A,Ellmerich S,Carroll MC,Heyman B

15-0452 was used in Flow cytometry/Cell sorting to investigate the role of the classical pathway activator IgM in complement activated antibody responses.

Tue Oct 25 00:00:00 EDT 2011

PAD4-mediated neutrophil extracellular trap formation is not required for immunity against influenza infection.

PloS one

Hemmers S,Teijaro JR,Arandjelovic S,Mowen KA

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2011

Type I interferon drives dendritic cell apoptosis via multiple BH3-only proteins following activation by PolyIC in vivo.

PloS one

Fuertes Marraco SA,Scott CL,Bouillet P,Ives A,Masina S,Vremec D,Jansen ES,O'Reilly LA,Schneider P,Fasel N,Shortman K,Strasser A,Acha-Orbea H

15-0452 was used in Flow cytometry/Cell sorting to elucidate the possible apoptotic mechanisms that control activated dendritic cell lifespan in response to PAMPs.

Fri Sep 30 00:00:00 EDT 2011

An information theoretic, microfluidic-based single cell analysis permits identification of subpopulations among putatively homogeneous stem cells.

PloS one

Glotzbach JP,Januszyk M,Vial IN,Wong VW,Gelbard A,Kalisky T,Thangarajah H,Longaker MT,Quake SR,Chu G,Gurtner GC

15-0452 was used in Flow cytometry/Cell sorting to develop a novel method to characterize and predict transcriptional programs across individual homogeneous stem cells.

Wed Nov 02 00:00:00 EDT 2011

In vivo requirement for Atg5 in antigen presentation by dendritic cells.

Immunity

Lee HK,Mattei LM,Steinberg BE,Alberts P,Lee YH,Chervonsky A,Mizushima N,Grinstein S,Iwasaki A

15-0452 was used in Flow cytometry/Cell sorting to investigate how dendritic cells utilise autophagic machinery to optimally present extracellular microbial antigens for MHC II molecules.

Fri Feb 26 00:00:00 EST 2010

Gas6 deficiency in recipient mice of allogeneic transplantation alleviates hepatic graft-versus-host disease.

Blood

Burnier L,Saller F,Kadi L,Brisset AC,Sugamele R,Baudino L,Bono F,Herbert JM,Carmeliet P,Schapira M,Izui S,Angelillo-Scherrer A

15-0452 was used in Flow cytometry/Cell sorting to study the effects of growth arrest-specific gene 6 deficiency on hepatic graft-versus-host disease in recipient mice of allogeneic transplantation.

Thu Apr 22 00:00:00 EDT 2010

Nonhematopoietic antigen blocks memory programming of alloreactive CD8+ T cells and drives their eventual exhaustion in mouse models of bone marrow transplantation.

The Journal of clinical investigation

Flutter B,Edwards N,Fallah-Arani F,Henderson S,Chai JG,Sivakumaran S,Ghorashian S,Bennett CL,Freeman GJ,Sykes M,Chakraverty R

15-0452 was used in Flow cytometry/Cell sorting to explore the role of recipient antigen expression by nonhaematopoietic cells in the failure to sustain effective cytotoxic T lymphocyte immunity following blood transplantation.

Mon Nov 01 00:00:00 EDT 2010

Additive and global functions of HoxA cluster genes in mesoderm derivatives.

Developmental biology

Di-Poï N,Koch U,Radtke F,Duboule D

15-0452 was used in Flow cytometry/Cell sorting to report that mutant mice lacking the entire HoxA cluster in mesodermal lineages display the expected spectrum of postnatal respiratory, cardiac and urogenital defects, previously reported for single gene mutations.

Sat May 15 00:00:00 EDT 2010

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

IL-7 specifies B cell fate at the common lymphoid progenitor to pre-proB transition stage by maintaining early B cell factor expression.

Journal of immunology (Baltimore, Md. : 1950)

Kikuchi K,Kasai H,Watanabe A,Lai AY,Kondo M

15-0452 was used in Flow cytometry/Cell sorting to investigate when IL-7 stimulation is necessary in hemato-/lymphopoiesis in adult mice, showing that IL-7 specifies B cell fate at the common lymphoid progenitor to pre-proB transition stage by maintaining early B cell factor expression.

Tue Jul 01 00:00:00 EDT 2008

p27 deficiency cooperates with Bcl-2 but not Bax to promote T-cell lymphoma.

PloS one

Cheng N,van de Wetering CI,Knudson CM

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Wed Apr 02 00:00:00 EDT 2008

Flow-cytometric phosphoprotein analysis reveals agonist and temporal differences in responses of murine hematopoietic stem/progenitor cells.

PloS one

Kalaitzidis D,Neel BG

15-0452 was used in Flow cytometry/Cell sorting to develop a method to study intracellular signaling in murine HSC/progenitor cell (HPC)-enriched populations, showing agonist and temporal differences in responses of HSC/HPC.

Tue Feb 10 00:00:00 EST 2009

Stable form of JAB1 enhances proliferation and maintenance of hematopoietic progenitors.

The Journal of biological chemistry

Mori M,Yoneda-Kato N,Yoshida A,Kato JY

15-0452 was used in Flow cytometry/Cell sorting to investigate how the expression and function of JAB1 are critical for the proliferation and maintenance of hematopoietic progenitors.

Fri Oct 24 00:00:00 EDT 2008

The impact of altered p53 dosage on hematopoietic stem cell dynamics during aging.

Blood

Dumble M,Moore L,Chambers SM,Geiger H,Van Zant G,Goodell MA,Donehower LA

15-0452 was used in Flow cytometry/Cell sorting to study how altering p53 dosage during ageing impacts hematopoietic stem cell dynamics.

Thu Feb 15 00:00:00 EST 2007

miR-150, a microRNA expressed in mature B and T cells, blocks early B cell development when expressed prematurely.

Proceedings of the National Academy of Sciences of the United States of America

Zhou B,Wang S,Mayr C,Bartel DP,Lodish HF

15-0452 was used in Immunofluorescence to study how miR-150 can down-regulate mRNAs important for B cell function, and how it can block further B cell development when expressed prematurely.

Tue Apr 24 00:00:00 EDT 2007

miR-150, a microRNA expressed in mature B and T cells, blocks early B cell development when expressed prematurely.

Proceedings of the National Academy of Sciences of the United States of America

Zhou B,Wang S,Mayr C,Bartel DP,Lodish HF

15-0452 was used in Immunofluorescence to study how miR-150 can down-regulate mRNAs important for B cell function, and how it can block further B cell development when expressed prematurely.

Tue Apr 24 00:00:00 EDT 2007

Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.

Molecular and cellular biology

Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2007

Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.

Molecular and cellular biology

Bai L,Chen Y,He Y,Dai X,Lin X,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2007

Hypersensitivity and loss of disease site targeting caused by antibody responses to PEGylated liposomes.

Molecular therapy : the journal of the American Society of Gene Therapy

Judge A,McClintock K,Phelps JR,Maclachlan I

15-0452 was used in Flow cytometry/Cell sorting to report that the in vivo efficacy and safety of PEGylated liposomes carrying therapeutic plasmid DNA can be severely compromised following repeat administration.

Wed Feb 01 00:00:00 EST 2006

Developmental switch of mouse hematopoietic stem cells from fetal to adult type occurs in bone marrow after birth.

Proceedings of the National Academy of Sciences of the United States of America

Kikuchi K,Kondo M

15-0452 was used in Flow cytometry/Cell sorting to demonstrate how foetal haematopoietic stem cells may acquire adult characteristics between 1 and 2 weeks after birth in mouse bone marrow.

Tue Nov 21 00:00:00 EST 2006

Essential role of phospholipase C gamma 2 in early B-cell development and Myc-mediated lymphomagenesis.

Molecular and cellular biology

Wen R,Chen Y,Bai L,Fu G,Schuman J,Dai X,Zeng H,Yang C,Stephan RP,Cleveland JL,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2006

Distinct roles of phosphoinositide-3 kinase and phospholipase Cgamma2 in B-cell receptor-mediated signal transduction.

Molecular and cellular biology

Dai X,Chen Y,Schuman J,Hua Z,Adamson JW,Wen R,Wang D

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2006

A role for brain-derived neurotrophic factor in B cell development.

Journal of neuroimmunology

Schuhmann B,Dietrich A,Sel S,Hahn C,Klingenspor M,Lommatzsch M,Gudermann T,Braun A,Renz H,Nockher WA

Wed Jun 01 00:00:00 EDT 2005

Beta-catenin is dispensable for hematopoiesis and lymphopoiesis.

The Journal of experimental medicine

Cobas M,Wilson A,Ernst B,Mancini SJ,MacDonald HR,Kemler R,Radtke F

15-0452 was used in Flow cytometry/Cell sorting to investigate an essential role for beta-catenin during hematopoiesis and lymphopoiesis.

Mon Jan 19 00:00:00 EST 2004

Impaired immune responses and B-cell proliferation in mice lacking the Id3 gene.

Molecular and cellular biology

Pan L,Sato S,Frederick JP,Sun XH,Zhuang Y

Published figure using CD45R (B220) monoclonal antibody (Product # 15-0452-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; loc; Ly-5; LY5; Lyt-4; PTPRC; T200

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UniProt ID: (Human) P08575, (Mouse) P06800

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Entrez Gene ID: (Human) 5788, (Mouse) 19264

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding phosphoprotein phosphatase activity heparin binding hydrolase activity phosphatase activity heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation activation of MAPK activity positive regulation of T cell mediated cytotoxicity positive regulation of T cell mediated immunity regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of humoral immune response mediated by circulating immunoglobulin leukocyte cell-cell adhesion response to gamma radiation B cell differentiation negative regulation of protein autophosphorylation heterotypic cell-cell adhesion T cell proliferation positive regulation of MAPK cascade positive thymic T cell selection negative thymic T cell selection regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of alpha-beta T cell proliferation positive regulation of isotype switching to IgG isotypes negative regulation of peptidyl-tyrosine phosphorylation regulation of B cell receptor signaling pathway regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway

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CD45R (B220) Monoclonal Antibody (RA3-6B2), PE-Cyanine5, eBioscience™

CD45R (B220) Antibody (15-0452-82) in Flow

Product Details

15-0452-82

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

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