Invitrogen

CD45 Monoclonal Antibody (HI30), Pacific Orange™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

29 References

View all (406) CD45 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (3)
Advanced Verification (1)

FIGURE: 1 / 4

CD45 Antibody (MHCD4530) in Flow

Human PBMCs stained with: CD45 Pacific Orange™ (Product # MHCD4530TR), CD4 PerCP-Cy®5.5 (Product # A15858), CD20 APC, CD14 APC-Cy®7 (Product # A15453), CD19 Pacific Green™ (Product # C11210), CD3 Alexa Fluor® 700 (CD0329), HLA-DR PE-Cy®7 (Product # A18558), CD8 Pacific Blue™ (Product # MHCD0828), CD33 FITC (Product # A16185), and CD11c PE (Product # A18674) using the Attune® NxT Acoustic Focusing Cytometer with 405 nm excitation and 440/50 emission filter (Pacific Blue™), 512/25 emission filter (Pacific Green™), and 603/48 emission filter ... View More

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

Product Details

MHCD4530

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 21 publications 21 publications

Miscellaneous PubMed (Misc)

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HI30

Immunogen

CD45 antigen

Conjugate

Pacific Orange™ Pacific Orange™ Pacific Orange™

Excitation/Emission Max

400/551 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10376143

Product Specific Information

Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Analyte Specific Reagent

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Product References

Improving NK cell function in multiple myeloma with NKTR-255, a novel polymer-conjugated human IL-15.

Blood advances

Fernandez RA,Mayoral JE,Pierre-Louis L,Yao Y,Xu Y,Mu S,Martinez-Lopez J,Primo D,Miyazaki T,Prabhala R,Anderson KC,Overwijk WW,Munshi NC,Fulciniti M

MHCD4530 was used in Flow cytometry/Cell sorting to evaluate the ability of the investigational medicine NKTR-255, an IL-15 receptor agonist, to engage the IL-15 pathway and stimulate NK cells against Multiple myeloma cells.

Tue Jan 10 00:00:00 EST 2023

Enhancer Hijacking Drives Oncogenic BCL11B Expression in Lineage-Ambiguous Stem Cell Leukemia.

Cancer discovery

Montefiori LE,Bendig S,Gu Z,Chen X,Pölönen P,Ma X,Murison A,Zeng A,Garcia-Prat L,Dickerson K,Iacobucci I,Abdelhamed S,Hiltenbrand R,Mead PE,Mehr CM,Xu B,Cheng Z,Chang TC,Westover T,Ma J,Stengel A,Kimura S,Qu C,Valentine MB,Rashkovan M,Luger S,Litzow MR,Rowe JM,den Boer ML,Wang V,Yin J,Kornblau SM,Hunger SP,Loh ML,Pui CH,Yang W,Crews KR,Roberts KG,Yang JJ,Relling MV,Evans WE,Stock W,Paietta EM,Ferrando AA,Zhang J,Kern W,Haferlach T,Wu G,Dick JE,Klco JM,Haferlach C,Mullighan CG

MHCD4530 was used in Flow cytometry/Cell sorting to identify oncogenic deregulation of BCL11B driven by diverse structural alterations, including de novo superenhancer generation, as the driving feature of a subset of lineage-ambiguous leukemias that transcend current diagnostic boundaries.

Mon Nov 01 00:00:00 EDT 2021

Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.

Cancer discovery

Du W,Hua F,Li X,Zhang J,Li S,Wang W,Zhou J,Wang W,Liao P,Yan Y,Li G,Wei S,Grove S,Vatan L,Zgodziński W,Majewski M,Wallner G,Chen H,Kryczek I,Fang JY,Zou W

MHCD4530 was used in Flow Cytometry to show that optineurin is a shared node between the IFN and MHC pathways and predicts colorectal cancer patient outcome.

Thu Jul 01 00:00:00 EDT 2021

Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity.

Cell reports

Szodoray P,Andersen TK,Heinzelbecker J,Imbery JF,Huszthy PC,Stanford SM,Bogen B,Landsverk OB,Bottini N,Tveita A,Munthe LA,Nakken B

MHCD4530 was used in Flow Cytometry to show that high CD45 phosphatase activity in memory B cells enhances B cell receptor signaling and is essential for their effective antibody-secreting cell differentiation.

Tue Aug 10 00:00:00 EDT 2021

Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?

Frontiers in immunology

Ehlers AM,den Hartog Jager CF,Kardol-Hoefnagel T,Katsburg MMD,Knulst AC,Otten HG

MHCD4530 was used in Flow cytometry/Cell sorting to compare strategies to generate human mAbs from peripheral blood regarding costs, time expenditure, recovery, throughput and complexity.

Mon Oct 18 00:00:00 EDT 2021

Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples.

Journal of immunological methods

van der Velden VHJ,Flores-Montero J,Perez-Andres M,Martin-Ayuso M,Crespo O,Blanco E,Kalina T,Philippé J,Bonroy C,de Bie M,Te Marvelde J,Teodosio C,Corral Mateos A,Kanderová V,van der Burg M,Van Hoof D,van Dongen JJM,Orfao A

MHCD4530 was used in flow cytometry to assess the use of dried formats of the lymphocytosis screening tube and of the primary immune deficiency orientation tube

Sun Dec 01 00:00:00 EST 2019

Influence of nasal polyp tissue on the differentiation and activation of T lymphocytes in a co-culture system.

Biomedical reports

Ickrath P,Scherzad A,Kleinsasser N,Ginzkey C,Hagen R,Hackenberg S

MHCD4530 was used in Flow cytometry/Cell sorting to assess the modulatory influence of the inflamed nasal polyp epithelial cells on the phenotype of T cells.

Fri Feb 01 00:00:00 EST 2019

Scalable high-throughput acoustophoresis in arrayed plastic microchannels.

Biomicrofluidics

Dubay R,Lissandrello C,Swierk P,Moore N,Doty D,Fiering J

MHCD4530TR was used in Flow cytometry/Cell sorting to suggest that acoustophoresis could in the future aid in cell therapy bioprocessing and that further scale-up is possible.

Wed May 01 00:00:00 EDT 2019

Maturation-associated gene expression profiles during normal human bone marrow erythropoiesis.

Cell death discovery

Mello FV,Land MGP,Costa ES,Teodósio C,Sanchez ML,Bárcena P,Peres RT,Pedreira CE,Alves LR,Orfao A

MHCD4530 was used in Flow Cytometry to investigate the ex vivo gene expression profile of normal bone marrow human erythroid precursor cells at different maturation stages.

Thu Oct 01 00:00:00 EDT 2020

Immunodeficiency in Bloom's Syndrome.

Journal of clinical immunology

Schoenaker MHD,Henriet SS,Zonderland J,van Deuren M,Pan-Hammarström Q,Posthumus-van Sluijs SJ,Pico-Knijnenburg I,Weemaes CMR,IJspeert H

MHCD4530 was used in Flow cytometry/Cell sorting to perform immune phenotyping of the B and T cells and analyzed the SHM and CSR in detail by analyzing IGHA and IGHG transcripts using next-generation sequencing.

Mon Jan 01 00:00:00 EST 2018

Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis.

International journal of molecular medicine

Ickrath P,Kleinsasser N,Ding X,Ginzkey C,Beyersdorf N,Hagen R,Kerkau T,Hackenberg S

MHCD4530 was used in Flow cytometry/Cell sorting to characterise CD69+ sphingosine-1-phosphate receptor 1 tissue resident memory T cells in the polyps of patients with chronic rhinosinusitis with nasal polyps.

Wed Aug 01 00:00:00 EDT 2018

Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.

Scientific reports

Mieczkowska A,Schumacher A,Filipowicz N,Wardowska A,Zieliński M,Madanecki P,Nowicka E,Langa P,Deptuła M,Zieliński J,Kondej K,Renkielska A,Buckley PG,Crossman DK,Crowley MR,Czupryn A,Mucha P,Sachadyn P,Janus Ł,Skowron P,Rodziewicz-Motowidło S,Cichorek M,Pikuła M,Piotrowski A

MHCD4530 was used in Flow cytometry/Cell sorting to study the impact of prolonged cell culture on the adipose-derived stem cell phenotype and their differentiation potential.

Fri Jul 27 00:00:00 EDT 2018

Targeting of colony-stimulating factor 1 receptor (CSF1R) in the CLL microenvironment yields antineoplastic activity in primary patient samples.

Oncotarget

Edwards V DK,Sweeney DT,Ho H,Eide CA,Rofelty A,Agarwal A,Liu SQ,Danilov AV,Lee P,Chantry D,McWeeney SK,Druker BJ,Tyner JW,Spurgeon SE,Loriaux MM

MHCD4530 was used in Flow cytometry/Cell sorting to demonstrate the preclinical activity of two inhibitors of CSF1R, GW-2580 and ARRY-382, in primary chronic lymphocytic leukaemia patient samples.

Tue May 15 00:00:00 EDT 2018

The IgE and basophil responses to Ara h 2 and Ara h 6 are good predictors of peanut allergy in children.

The Journal of allergy and clinical immunology

van Erp FC,Knol EF,Pontoppidan B,Meijer Y,van der Ent CK,Knulst AC

MHCD4530 was used in flow cytometry to identify peanut proteins that can predict peanut allergy.

Sun Jan 01 00:00:00 EST 2017

Genetic defects in PI3Kδ affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections.

Clinical immunology (Orlando, Fla.)

Wentink M,Dalm V,Lankester AC,van Schouwenburg PA,Schölvinck L,Kalina T,Zachova R,Sediva A,Lambeck A,Pico-Knijnenburg I,van Dongen JJ,Pac M,Bernatowska E,van Hagen M,Driessen G,van der Burg M

MHCD4530 was used in flow cytometry to discuss how mutations in PIK3CD and PIK3R1 cause activated PI3K-delta syndrome

Wed Mar 01 00:00:00 EST 2017

Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.

Mucosal immunology

Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M

MHCD4530 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.

Fri Jan 01 00:00:00 EST 2016

Immune response to JC virus T antigen in patients with and without colorectal neoplasia.

Gut microbes

Butcher LD,Garcia M,Arnold M,Ueno H,Goel A,Boland CR

MHCD4530 was used in flow cytometry to study the cell mediated immune response to JCV T-Ag in patients with colorectal adenomatous polyps or cancers

Tue Jul 01 00:00:00 EDT 2014

Human plasmacytoid dendritic cells regulate IFN-α production through activation-induced splicing of IL-18Rα.

Journal of leukocyte biology

Chao Y,Kaliaperumal N,Chretien AS,Tang S,Lee B,Poidinger M,Fairhurst AM,Connolly JE

MHCD4530 was used in Flow cytometry/Cell sorting to explore the role of IL-18R signalling in IFN-α production in human plasmacytoid dendritic cells.

Mon Dec 01 00:00:00 EST 2014

Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice.

Journal of immunology (Baltimore, Md. : 1950)

Billerbeck E,Horwitz JA,Labitt RN,Donovan BM,Vega K,Budell WC,Koo GC,Rice CM,Ploss A

MHCD4530 was used in flow cytometry to investigate the role of HLA molecules during infection using humanized mice

Thu Aug 15 00:00:00 EDT 2013

FcRL5 as a target of antibody-drug conjugates for the treatment of multiple myeloma.

Molecular cancer therapeutics

Elkins K,Zheng B,Go M,Slaga D,Du C,Scales SJ,Yu SF,McBride J,de Tute R,Rawstron A,Jack AS,Ebens A,Polson AG

MHCD4530 was used in flow cytometry to investigate the use of FcRL5 antibody-drug conjugates as a treatment for multiple myeloma.

Mon Oct 01 00:00:00 EDT 2012

In vitro immunomodulation of a whole blood IFN-γ release assay enhances T cell responses in subjects with latent tuberculosis infection.

PloS one

Gaur RL,Suhosk MM,Banaei N

MHCD4530 was used in flow cytometry to discuss the use of PPR ligands as adjuvants and the use of interferon-gamma release assays as a diagnostic tool

Tue Aug 26 00:00:00 EDT 2014

Rapid ex vivo isolation and long-term culture of human Th17 cells.

Journal of immunological methods

Streeck H,Cohen KW,Jolin JS,Brockman MA,Meier A,Power KA,Waring MT,Alter G,Altfeld M

MHCD4530 was used in flow cytometry to describe a novel technique to isolate viable Th17 cells based on their ability to secrete IL-17.

Sun Apr 20 00:00:00 EDT 2008

Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.

European heart journal

Leone AM,Rutella S,Bonanno G,Abbate A,Rebuzzi AG,Giovannini S,Lombardi M,Galiuto L,Liuzzo G,Andreotti F,Lanza GA,Contemi AM,Leone G,Crea F

MHCD4530 was used in Flow cytometry/Cell sorting to assess the spontaneous mobilisation of bone marrow-derived stem cells expressing CD34 following acute myocardial infarction, and its relation to post-infarction remodelling.

Wed Jun 01 00:00:00 EDT 2005

Analysis of CD10+ hairy cell leukemia.

American journal of clinical pathology

Jasionowski TM,Hartung L,Greenwood JH,Perkins SL,Bahler DW

MHCD4530 was used in flow cytometry to examine CD10 expression in hairy cell leukemia.

Fri Aug 01 00:00:00 EDT 2003

Prospective isolation of human clonogenic common myeloid progenitors.

Proceedings of the National Academy of Sciences of the United States of America

Manz MG,Miyamoto T,Akashi K,Weissman IL

MHCD4530 was used in flow cytometry to report how to isolate highly purified hematopoietic intermediates.

Tue Sep 03 00:00:00 EDT 2002

Precise CD4 T-cell counting using red diode laser excitation: for richer, for poorer.

Cytometry

Janossy G,Jani IV,Kahan M,Barnett D,Mandy F,Shapiro H

MHCD4530 was used in flow cytometry to discuss the high cost of measuring CD4 T cells by flow cytometry.

Mon Apr 15 00:00:00 EDT 2002

Short-term, serum-free, static culture of cord blood-derived CD34+ cells: effects of FLT3-L and MIP-1alpha on in vitro expansion of hematopoietic progenitor cells.

Haematologica

Capmany G,Querol S,Cancelas JA,García J

MHCD4530 was used in flow cytometry to identify the optimal condition for the generation of committed progenitors from cord blood cells without affecting the stem cell compartment.

Sun Aug 01 00:00:00 EDT 1999

Illegitimate transcription: its use for studying genetic abnormalities in lymphoblastoid cells from patients with Glanzmann thrombasthenia.

British journal of haematology

Negrier C,Vinciguerra C,Attali O,Grenier C,Larcher ME,Dechavanne M

MHCD4530 was used in flow cytometry to study RNA transcripts in patients with Glanzmann thrombasthenia.

Thu Jan 01 00:00:00 EST 1998

Growth of human hematopoietic cells in immunodeficient mice conditioned with cyclophosphamide and busulfan.

Stem cells (Dayton, Ohio)

Basch RS,Quito FL,Beh J,Hirst JA

MHCD4530 was used in immunocytochemistry to report that human hematopoietic cells survive and proliferate in severe combined immunodeficient mice treated with busulfan and cyclophosphamide.

Thu Oct 16 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45 Monoclonal Antibody (HI30), Pacific Orange™

CD45 Antibody (MHCD4530) in Flow

Product Details

MHCD4530

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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